##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630964.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413293
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.949609599001192	31.0	30.0	34.0	28.0	34.0
2	30.942362924124048	31.0	31.0	34.0	27.0	34.0
3	30.37433975412117	31.0	30.0	34.0	26.0	34.0
4	34.60582443932029	35.0	35.0	37.0	30.0	37.0
5	34.71132102406767	35.0	35.0	37.0	32.0	37.0
6	34.935099312110296	35.0	35.0	37.0	32.0	37.0
7	34.95535370790214	35.0	35.0	37.0	32.0	37.0
8	34.99687146890947	36.0	35.0	37.0	32.0	37.0
9	36.681855245552185	38.0	35.0	39.0	32.0	39.0
10	36.458468931242486	38.0	35.0	39.0	32.0	39.0
11	36.54126733334463	38.0	35.0	39.0	32.0	39.0
12	36.44965436143366	38.0	35.0	39.0	32.0	39.0
13	36.57976060567201	38.0	35.0	39.0	32.0	39.0
14	37.52470281374231	39.0	36.0	40.0	32.0	41.0
15	37.53143895493028	39.0	36.0	40.0	32.0	41.0
16	37.524639904377764	39.0	36.0	40.0	32.0	41.0
17	37.504523425269724	39.0	36.0	40.0	32.0	41.0
18	37.5833053064049	39.0	37.0	40.0	32.0	41.0
19	37.57905408511637	39.0	37.0	40.0	32.0	41.0
20	37.58655239745168	39.0	37.0	40.0	32.0	41.0
21	37.53917196758716	39.0	37.0	40.0	32.0	41.0
22	37.41685922577929	39.0	36.0	40.0	32.0	41.0
23	37.453854771312365	39.0	36.0	40.0	32.0	41.0
24	37.38110734999141	39.0	36.0	40.0	32.0	41.0
25	37.342444706297954	39.0	36.0	40.0	32.0	41.0
26	37.16343852908227	39.0	36.0	40.0	31.0	41.0
27	37.068764774627205	39.0	36.0	40.0	31.0	41.0
28	37.02443302935206	39.0	36.0	40.0	31.0	41.0
29	36.95793057225745	39.0	36.0	40.0	31.0	41.0
30	36.85010392143104	39.0	36.0	40.0	31.0	41.0
31	36.764852054111735	39.0	36.0	40.0	30.0	41.0
32	36.65177247134599	38.0	35.0	40.0	30.0	41.0
33	36.51179187646537	38.0	35.0	40.0	30.0	41.0
34	36.4139315207371	38.0	35.0	40.0	30.0	41.0
35	36.408371300747895	38.0	35.0	40.0	30.0	41.0
36	36.339802512987156	38.0	35.0	40.0	30.0	41.0
37	36.28251385820713	38.0	35.0	40.0	30.0	41.0
38	36.22011018817159	38.0	35.0	40.0	30.0	41.0
39	36.083790434389165	38.0	35.0	40.0	29.0	41.0
40	35.88432903533329	38.0	35.0	40.0	28.0	41.0
41	35.86905657729504	38.0	35.0	40.0	28.0	41.0
42	35.77763959225054	38.0	34.0	40.0	28.0	41.0
43	34.97171498186517	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	8.0
11	14.0
12	5.0
13	15.0
14	8.0
15	16.0
16	7.0
17	16.0
18	22.0
19	30.0
20	75.0
21	145.0
22	317.0
23	564.0
24	994.0
25	1605.0
26	2372.0
27	3672.0
28	5428.0
29	7538.0
30	9726.0
31	12730.0
32	16440.0
33	21198.0
34	27622.0
35	36676.0
36	50702.0
37	71946.0
38	96640.0
39	46754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.84938530292069	24.396735487898415	16.70872722257575	23.045151986605145
2	13.565436627283791	22.760608091596033	40.36264829067998	23.311306990440194
3	14.971218965721656	29.72999784656406	34.95945975373404	20.33932343398025
4	10.86009199284769	19.55295637719487	37.814335108506555	31.772616521450885
5	11.722676164367652	39.09381479966997	35.32602778174322	13.857481254219161
6	26.249658232779165	40.709133713854335	17.46775290169444	15.573455151672059
7	22.712216272716933	34.67781936785767	22.681971385917496	19.927992973507898
8	25.28666103708507	33.799265896107606	23.47148391093002	17.442589155877307
9	22.961192180849903	14.722968934871872	19.640303610271648	42.67553527400658
10	13.984751737871196	28.89741660274914	33.70974103118127	23.408090628198398
11	30.580000145175458	22.941835453298264	25.124064525651292	21.354099875874986
12	19.63957773298846	27.71472054934393	28.480279124011293	24.165422593656317
13	29.508847234286574	20.29843234702741	26.4037861759091	23.788934242776914
14	21.28756112491622	23.51334283426044	27.158456591328672	28.04063944949467
15	24.08678588797778	29.831620666210167	22.589543011858414	23.492050433953633
16	24.104690860963046	27.51292666461808	23.8058713793846	24.576511095034274
17	20.09204123950805	31.574935941329755	24.83274577599911	23.500277043163084
18	22.694553258826065	25.02631305151551	27.210235837529307	25.06889785212912
19	22.908203139177292	28.46043847827086	28.512701642660293	20.118656739891556
20	21.67929289874254	28.661022567524736	25.766707880365747	23.892976653366983
21	24.7204767561996	27.1567628776679	26.857701436995065	21.26505892913744
22	22.39041067717092	31.620908169264904	26.73309250338138	19.2555886501828
23	24.65998698260072	25.53055580423574	26.73091487153182	23.078542341631724
24	22.62704667148972	27.92909630697834	27.599548020411667	21.844309001120273
25	23.04636178207712	29.630552658767506	25.94720936478479	21.37587619437058
26	22.436866823294853	29.27003360811821	28.48971552869272	19.803384039894215
27	22.108044414011367	31.400967352459393	26.924724106142616	19.566264127386624
28	20.960674388387897	28.30582661695214	28.836200951867074	21.897298042792883
29	22.78722359197954	26.38346161197988	28.536897552099845	22.29241724394074
30	24.077107524201956	27.50808748273017	28.645779144577816	19.769025848490056
31	21.125448531671235	28.252111697996334	26.075931603003195	24.546508167329232
32	26.13448570384691	26.17561874989414	27.967809762081625	19.722085784177327
33	21.201907605500214	28.093386532072888	30.14544161164114	20.559264250785763
34	20.899216778411443	29.111308442194762	27.05368830345542	22.93578647593838
35	22.10514090487862	25.418528743530615	32.6538315432393	19.82249880835146
36	22.219829515622088	27.831586791936957	26.355394357029997	23.593189335410955
37	21.21836082391911	26.397737198549216	30.074547596983255	22.309354380548424
38	21.28223802483952	24.572155831335156	29.489732465829327	24.655873677996
39	24.690715787588953	25.631936664787453	27.43791934535547	22.239428202268126
40	20.091557321319257	24.524731848833635	34.05429078160046	21.329420048246643
41	21.760591154459426	26.419755476139205	26.983036247891935	24.836617121509438
42	21.422332340494517	24.861538908232177	32.10893966266063	21.60718908861268
43	22.65825939466674	24.71926696072762	30.48418434379484	22.138289300810804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	14.5
2	17.0
3	79.0
4	141.0
5	141.0
6	211.0
7	281.0
8	272.5
9	264.0
10	384.5
11	505.0
12	505.0
13	780.0
14	1055.0
15	2503.5
16	3952.0
17	4357.5
18	4763.0
19	4763.0
20	4842.0
21	4921.0
22	5744.5
23	6568.0
24	8160.5
25	9753.0
26	9753.0
27	11191.0
28	12629.0
29	14245.0
30	15861.0
31	17575.5
32	19290.0
33	19290.0
34	20735.0
35	22180.0
36	23183.5
37	24187.0
38	24453.5
39	24720.0
40	24720.0
41	25015.0
42	25310.0
43	25352.5
44	25395.0
45	25324.0
46	25253.0
47	25253.0
48	26576.5
49	27900.0
50	34916.5
51	41933.0
52	32292.0
53	22651.0
54	22651.0
55	28731.5
56	34812.0
57	28433.0
58	22054.0
59	17039.0
60	12024.0
61	12024.0
62	10615.0
63	9206.0
64	7774.5
65	6343.0
66	5189.5
67	4036.0
68	4036.0
69	3316.5
70	2597.0
71	2124.0
72	1651.0
73	1097.5
74	544.0
75	544.0
76	378.5
77	213.0
78	173.0
79	133.0
80	103.5
81	74.0
82	74.0
83	57.5
84	41.0
85	27.5
86	14.0
87	11.0
88	8.0
89	8.0
90	5.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	413293.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.10070936325668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.62181546389914	49.46167109321758
2	8.03874710299831	9.180363239460561
3	1.8964370342327495	3.2486369975232203
4	0.823074962489595	1.8799265686916704
5	0.4943831278656681	1.4114813649177638
6	0.334790460276826	1.1470063661914784
7	0.2468113974770863	0.986517411241483
8	0.1948683197503912	0.8901695432138608
9	0.1369515317371414	0.7038026649517799
>10	1.0648058511151617	11.837125000752435
>50	0.10074974155642295	4.005262603016571
>100	0.040215232974063556	3.8147890404733182
>500	0.0021165912091612397	0.9117592358804559
>1k	0.0025399094509934877	3.0502358954335826
>5k	0.0016932729673289917	7.4712529750342025
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	9819	2.375796347869429	No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	8533	2.0646369524768144	RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	7366	1.7822706893172642	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	5191	1.2560096590070482	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	2760	0.6678071005315841	No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC	2708	0.655225227623018	RNA PCR Primer, Index 46 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2371	0.573685012811734	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2238	0.5415044532571324	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1289	0.3118852726758014	No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG	1253	0.3031747452775634	No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA	821	0.1986484164987067	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	817	0.19768058012112474	No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	764	0.18485674811816313	No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT	744	0.1800175662302531	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	626	0.15146639309158394	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	4.8391818879100297E-4	0.0
17	0.0	0.0	0.0	9.678363775820059E-4	0.0
18	0.0	0.0	0.0	0.0012097954719775075	0.0
19	0.0	0.0	0.0	0.0012097954719775075	0.0
20	0.0	0.0	0.0	0.001451754566373009	0.0
21	0.0	0.0	0.0	0.0016937136607685106	0.0
22	0.0	0.0	0.0	0.0019356727551640119	0.0
23	0.0	0.0	0.0	0.0026615500383505163	0.0
24	0.0	0.0	0.0	0.003387427321537021	0.0
25	0.0	0.0	0.0	0.0038713455103280238	0.0
26	0.0	0.0	0.0	0.0038713455103280238	0.0
27	0.0	0.0	0.0	0.0041133046047235255	0.0
28	0.0	0.0	0.0	0.007258772831865045	0.0
29	0.0	0.0	0.0	0.019840645740431123	0.0
30	0.0	0.0	0.0	0.034358191404161215	0.0
31	0.0	0.0	0.0	0.06194152816524838	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGA	20	0.0018405723	37.0	1
GCAACGC	25	1.2322576E-4	37.0	3
TATTCGC	20	0.0018405723	37.0	3
CTTATAC	965	0.0	32.97409	1
CGTCTTC	325	0.0	30.73846	37
TACTCGA	25	0.0054923207	29.6	5
CTCGAAC	25	0.0054923207	29.6	28
GGTATCA	340	0.0	28.294117	1
CCGTCTT	1320	0.0	28.170454	37
TTATACA	1140	0.0	28.074562	2
GATTACG	40	5.9310667E-5	27.75	1
TATACAC	1155	0.0	27.709957	3
GCCGTCT	1345	0.0	27.64684	36
GTATCAA	875	0.0	22.622856	1
TGCCGTC	1680	0.0	22.133928	35
ATGCCGT	1770	0.0	21.008474	34
AGCAACG	45	0.0038217246	20.555555	2
ATTACGG	55	5.135437E-4	20.181818	2
ATTGAGC	225	0.0	19.733334	6
CTAGCCA	85	1.2413639E-6	19.588234	9
>>END_MODULE