FastQCFastQC Report
Fri 10 Feb 2017
ERR1630963.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630963.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740774
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT142561.9244735911357578No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG107331.4488899448414767No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT102121.378558102741187RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC55350.7471914511038454No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC41180.5559050398637101RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC27390.36974839829691647RNA PCR Primer, Index 27 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17080.2305696474228307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16750.22611484744334978No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG11960.16145275077149038No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA11120.15011325991462984No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT8380.11312492069106098No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8090.10921009646666865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC16900.032.9497071
CGTCTTC5250.029.59999837
TATACAC19750.029.2253173
TTATACA19750.028.569622
CCGTCTT15250.025.23278637
GCCGTCT15650.024.46964836
AAACCCG652.6807083E-622.769235
GGTATCA3950.022.0126571
CGGATCG603.7239835E-521.58333437
GTATCAA8650.021.3872851
TTACGGG705.0978815E-621.1428593
GATTACG705.0978815E-621.1428591
TACACCG450.003824542520.5555575
TGCCGTC18950.020.20844335
ATGCCGT20300.019.04679934
GCGAACT500.0070325818.49999828
GCAACGC500.0070325818.4999983
TATGCCG21200.018.06367933
ATACACA32350.017.7279764
TAATAGG1156.3957486E-817.6956522
Produced by FastQC (version 0.11.5)