##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630963.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	740774
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.519790111423998	31.0	31.0	34.0	28.0	34.0
2	31.520218042209905	31.0	31.0	34.0	30.0	34.0
3	31.001632076719755	31.0	31.0	34.0	27.0	34.0
4	35.14039099644426	37.0	35.0	37.0	32.0	37.0
5	35.32163655851852	37.0	35.0	37.0	33.0	37.0
6	35.50571294348884	37.0	35.0	37.0	33.0	37.0
7	35.50740171766288	37.0	35.0	37.0	33.0	37.0
8	35.5319719644588	37.0	35.0	37.0	33.0	37.0
9	37.31180225007897	39.0	37.0	39.0	34.0	39.0
10	37.14588929957045	39.0	37.0	39.0	33.0	39.0
11	37.21142615696555	39.0	37.0	39.0	33.0	39.0
12	37.13296227999363	39.0	37.0	39.0	33.0	39.0
13	37.21033810581905	39.0	37.0	39.0	34.0	39.0
14	38.362111791180574	40.0	38.0	41.0	34.0	41.0
15	38.35677278090214	40.0	38.0	41.0	34.0	41.0
16	38.361289678093456	40.0	38.0	41.0	34.0	41.0
17	38.31096798753736	40.0	38.0	41.0	33.0	41.0
18	38.37108888810892	40.0	38.0	41.0	34.0	41.0
19	38.39090059856312	40.0	38.0	41.0	34.0	41.0
20	38.353982456187715	40.0	38.0	41.0	34.0	41.0
21	38.28440253032639	40.0	38.0	41.0	34.0	41.0
22	38.249513346850726	40.0	38.0	41.0	34.0	41.0
23	38.24964564091072	40.0	38.0	41.0	34.0	41.0
24	38.226563027320076	40.0	38.0	41.0	33.0	41.0
25	38.18953014009671	40.0	38.0	41.0	33.0	41.0
26	38.066857098116294	40.0	37.0	41.0	33.0	41.0
27	38.017766552281806	40.0	37.0	41.0	33.0	41.0
28	37.96102050017954	40.0	37.0	41.0	33.0	41.0
29	37.953292097184836	40.0	37.0	41.0	33.0	41.0
30	37.87748355098856	40.0	37.0	41.0	33.0	41.0
31	37.77921066344121	40.0	37.0	41.0	33.0	41.0
32	37.75925450947253	40.0	37.0	41.0	33.0	41.0
33	37.74082648689074	40.0	37.0	41.0	33.0	41.0
34	37.67979437723246	40.0	37.0	41.0	33.0	41.0
35	37.68112946728692	40.0	37.0	41.0	33.0	41.0
36	37.640629935715886	40.0	37.0	41.0	33.0	41.0
37	37.58405667585525	40.0	37.0	41.0	32.0	41.0
38	37.53935613290963	40.0	37.0	41.0	32.0	41.0
39	37.45229719185608	40.0	37.0	41.0	32.0	41.0
40	37.36023672537103	39.0	37.0	41.0	31.0	41.0
41	37.329877938480564	39.0	36.0	41.0	31.0	41.0
42	37.26194493867225	39.0	36.0	41.0	31.0	41.0
43	36.60403847867231	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	15.0
11	19.0
12	15.0
13	7.0
14	7.0
15	7.0
16	8.0
17	17.0
18	15.0
19	33.0
20	59.0
21	125.0
22	246.0
23	447.0
24	906.0
25	1550.0
26	2504.0
27	3904.0
28	6041.0
29	8546.0
30	11954.0
31	16081.0
32	20769.0
33	27199.0
34	35883.0
35	48377.0
36	67963.0
37	99820.0
38	176180.0
39	212065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.41060026404814	22.345006709198756	15.140380196929156	25.10401282982394
2	13.48778439848051	21.54692254317781	41.288841131033216	23.676451927308463
3	14.439626660762933	28.142051421891157	34.3504226660223	23.067899251323613
4	11.725033545993785	19.431702516557007	37.432064300312916	31.411199637136296
5	12.677145796153752	37.89131368001576	35.75030981108948	13.681230712740996
6	28.056195276832067	39.893138798067966	17.77411194237379	14.276553982726176
7	24.494515196267688	33.24009751962137	22.750933483086612	19.514453801024334
8	24.603725292734357	35.54012424842124	22.122671692041028	17.73347876680337
9	23.607605018534667	14.955438500811313	19.74961324236542	41.6873432382886
10	14.263594564604048	28.865078957954786	33.513595239573746	23.35773123786742
11	31.33438808597494	22.68816130155756	25.648578378830788	20.328872233636712
12	20.398394112104366	26.071244401126393	29.58405667585525	23.94630481091399
13	28.801496812793104	20.69659572285204	26.734874604130276	23.767032860224578
14	21.555832143136776	22.192463558386226	26.469341526565458	29.78236277191154
15	23.85531889618156	28.79366716434432	22.706655471169345	24.644358468304773
16	25.058924854274046	27.3527418618904	23.675101987920744	23.913231295914812
17	20.892606921949206	30.58868156819759	25.326077859104124	23.19263365074908
18	24.246396336804477	25.77965749337855	25.754413626828153	24.21953254298882
19	25.00546725452027	27.214238080710174	25.41868370110182	22.36161096366773
20	23.5008248129659	26.434378096423472	25.48213085232473	24.5826662382859
21	24.009886956075672	26.979078639369092	25.533158561180603	23.477875843374633
22	21.478480616220335	29.521554482203747	26.202998485368006	22.796966416207912
23	22.90428659753177	25.55948237924117	27.16591025062975	24.37032077259731
24	23.88933736875214	27.04225580271446	25.421248585938493	23.647158242594905
25	22.2706250489353	27.427798491847717	26.531168750523104	23.77040770869388
26	24.822820455361555	26.798321755353182	26.961529427328713	21.417328361956546
27	22.88903228245052	28.720230461652275	25.917621298803684	22.473115957093526
28	21.150715332881553	26.794406931128794	28.27731534854085	23.777562387448803
29	23.615434666983454	25.616044839586706	25.925585941191244	24.842934552238603
30	23.15348540850516	25.55124774897607	28.9696992605032	22.32556758201557
31	22.18881872203938	26.89646234884054	26.37093094520056	24.543787983919522
32	22.091623086123434	25.81799577198984	26.987178275695424	25.10320286619131
33	20.905836327948876	25.609835118403186	31.742474762883145	21.741853790764797
34	21.304203441265486	27.74327932675823	26.522124156625367	24.430393075350917
35	21.622789136767757	25.393709822428974	29.870648807868527	23.112852232934742
36	23.116362075342817	25.59701069421983	27.41105924343997	23.875567986997385
37	22.209742782549064	26.975973778777334	27.59843083045571	23.21585260821789
38	22.17005456455005	25.203233374821473	28.18551947017579	24.44119259045269
39	24.059699719482595	25.6111850577909	26.560867417052975	23.768247805673525
40	21.312708059408134	25.095373217742523	31.302394522485937	22.289524200363402
41	22.562211956683146	26.012387043821732	26.281969939549715	25.14343105994541
42	23.045085275671124	25.0668894966616	29.50157537926547	22.386449848401806
43	22.95882414879572	24.703080831670658	29.331483016412562	23.00661200312106
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15.0
1	18.5
2	22.0
3	75.0
4	128.0
5	128.0
6	153.5
7	179.0
8	212.5
9	246.0
10	325.5
11	405.0
12	405.0
13	641.0
14	877.0
15	1946.0
16	3015.0
17	3436.0
18	3857.0
19	3857.0
20	4117.0
21	4377.0
22	5591.5
23	6806.0
24	9109.5
25	11413.0
26	11413.0
27	14284.0
28	17155.0
29	20519.5
30	23884.0
31	27492.0
32	31100.0
33	31100.0
34	35433.0
35	39766.0
36	42412.0
37	45058.0
38	47671.5
39	50285.0
40	50285.0
41	51236.5
42	52188.0
43	52806.0
44	53424.0
45	53526.0
46	53628.0
47	53628.0
48	52843.0
49	52058.0
50	51212.5
51	50367.0
52	60122.5
53	69878.0
54	69878.0
55	55024.5
56	40171.0
57	43903.5
58	47636.0
59	39915.0
60	32194.0
61	32194.0
62	25220.0
63	18246.0
64	15208.5
65	12171.0
66	10138.0
67	8105.0
68	8105.0
69	6694.5
70	5284.0
71	4201.5
72	3119.0
73	2421.5
74	1724.0
75	1724.0
76	1334.5
77	945.0
78	743.5
79	542.0
80	396.5
81	251.0
82	251.0
83	199.5
84	148.0
85	109.0
86	70.0
87	49.0
88	28.0
89	28.0
90	18.0
91	8.0
92	4.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	740774.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.675474985077315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.388099763079	49.38261611257676
2	11.382222055705908	13.812434592311584
3	3.155967950318487	5.744695632697653
4	1.3401073131686747	3.2524659102994042
5	0.704123034964315	2.136149979719701
6	0.44224527386064255	1.6100065230840048
7	0.2959733875595803	1.2570828111183936
8	0.21206555472446298	1.029374260870456
9	0.16663734116939027	0.909971984313282
>10	0.841075097610134	9.203253564877482
>50	0.04750768822918822	1.9160842113070182
>100	0.020867599291248238	2.1676783050581596
>500	8.879829482759153E-4	0.4009928734776745
>1k	0.0013319744224138726	1.690177553375163
>5k	2.2199573706897883E-4	0.7455477173997073
>10k+	6.659872112069363E-4	4.7414679675134765
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	14256	1.9244735911357578	No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG	10733	1.4488899448414767	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	10212	1.378558102741187	RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC	5535	0.7471914511038454	No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC	4118	0.5559050398637101	RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC	2739	0.36974839829691647	RNA PCR Primer, Index 27 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1708	0.2305696474228307	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1675	0.22611484744334978	No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG	1196	0.16145275077149038	No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA	1112	0.15011325991462984	No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT	838	0.11312492069106098	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	809	0.10921009646666865	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.3499393877214913E-4	0.0
10	0.0	0.0	0.0	1.3499393877214913E-4	0.0
11	0.0	0.0	0.0	1.3499393877214913E-4	0.0
12	0.0	0.0	0.0	2.6998787754429826E-4	0.0
13	0.0	0.0	0.0	4.049818163164474E-4	0.0
14	0.0	0.0	0.0	4.049818163164474E-4	0.0
15	0.0	0.0	0.0	4.049818163164474E-4	0.0
16	0.0	0.0	0.0	4.049818163164474E-4	0.0
17	0.0	0.0	0.0	4.049818163164474E-4	0.0
18	0.0	0.0	0.0	5.399757550885965E-4	0.0
19	0.0	0.0	0.0	8.099636326328948E-4	0.0
20	0.0	0.0	0.0	8.099636326328948E-4	0.0
21	0.0	0.0	0.0	9.449575714050439E-4	0.0
22	0.0	0.0	0.0	0.0013499393877214913	0.0
23	0.0	0.0	0.0	0.0016199272652657895	0.0
24	0.0	0.0	0.0	0.002159903020354386	0.0
25	0.0	0.0	0.0	0.0024298908978986843	0.0
26	0.0	0.0	0.0	0.0025648848366708337	0.0
27	0.0	0.0	0.0	0.0037798302856201756	0.0
28	0.0	0.0	0.0	0.008234630265101097	0.0
29	0.0	0.0	0.0	0.01673924840774649	0.0
30	0.0	0.0	0.0	0.02767375744829057	0.0
31	0.0	0.0	0.0	0.06628202393712522	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1690	0.0	32.949707	1
CGTCTTC	525	0.0	29.599998	37
TATACAC	1975	0.0	29.225317	3
TTATACA	1975	0.0	28.56962	2
CCGTCTT	1525	0.0	25.232786	37
GCCGTCT	1565	0.0	24.469648	36
AAACCCG	65	2.6807083E-6	22.76923	5
GGTATCA	395	0.0	22.012657	1
CGGATCG	60	3.7239835E-5	21.583334	37
GTATCAA	865	0.0	21.387285	1
TTACGGG	70	5.0978815E-6	21.142859	3
GATTACG	70	5.0978815E-6	21.142859	1
TACACCG	45	0.0038245425	20.555557	5
TGCCGTC	1895	0.0	20.208443	35
ATGCCGT	2030	0.0	19.046799	34
GCGAACT	50	0.00703258	18.499998	28
GCAACGC	50	0.00703258	18.499998	3
TATGCCG	2120	0.0	18.063679	33
ATACACA	3235	0.0	17.727976	4
TAATAGG	115	6.3957486E-8	17.695652	2
>>END_MODULE