FastQCFastQC Report
Fri 10 Feb 2017
ERR1630962.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630962.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406694
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT242905.972549386024874No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG190574.68583259157991No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT137423.378953217898469Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC98362.4185259679267457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59151.454410441265423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58541.4394114493943848No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC54531.340811519225757Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC46791.15049644204242No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA45821.1266455861163431No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA26480.6511037782706408No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT23770.5844689127452088No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG22930.5638145632834515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22570.5549626992284125No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT19600.48193482077434135No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT19030.4679193693538631No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA18950.46595228845274334No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG18940.4657064033401033No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC18100.4450520538783459No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA18020.44308497297722615No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG16070.395137376012432No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC15530.38185957992987357No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT14810.3641558518197957No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG14060.3457144683717979No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG11980.2945703649426842No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT11770.2894067775772448No Hit
ACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT11630.28596438600028523No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG11340.27883371773372606No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG11060.27194893457980696No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG10620.26112998962364825No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG10460.2571958278214087No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10440.2567040575961288No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG10380.25522874692028896No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG9720.23900032948605093No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG9460.2326073165574117No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9180.22572253340349255No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8910.21908363536221337No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8490.2087564606313346No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC8460.20801880529341474No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT8380.20605172439229497No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8140.20015048168893568No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG7650.18810211116957712No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7120.17507020019965872No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA7030.17285723418589896No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6990.17187369373533912No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC6650.16351359990558012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.16253005945502025No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA6540.16080886366654046No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG6510.16007120832862054No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6170.15171111449886154No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA6130.15072757404830167No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6100.14998991871038175No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG6030.14826872292190196No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG5890.14482633134494238No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG5880.14458044623230243No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5820.1431051355564626No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT5670.13941685886686303No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG5510.13548269706462351No Hit
ATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT5210.12810614368542442Illumina PCR Primer Index 10 (95% over 21bp)
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA5200.12786025857278444No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC5180.1273684883475045No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5170.12712260323486455No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5160.12687671812222456No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG5100.12540140744638476No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT5080.1249096372211048No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5060.12441786699582487No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4890.12023782008094537No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4890.12023782008094537No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC4870.11974604985566543No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC4790.11777896895454568No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4730.11630365827870585No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG4630.11384480715230616No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA4590.11286126670174627No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG4570.11236949647646632No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4570.11236949647646632No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA4520.1111400709132665No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.11089418580062652No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4500.11064830068798655No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4480.11015653046270661No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTG4470.10991064535006663No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA4390.10794356444894686No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4390.10794356444894686No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA4340.10671413888574703No Hit
TCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT4270.10499294309726723Illumina PCR Primer Index 10 (95% over 24bp)
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA4260.10474705798462726No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC4260.10474705798462726No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4120.10130466640766768No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA4090.10056701106974776No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG4080.10032112595710779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG308.298775E-637.02
ATTACGG800.037.02
ATGGTGA200.001840537837.02
GTATACA200.001840537837.02
TGGTGAC200.001840537837.03
ACTCAGA600.037.01
CGCCGAG200.001840537837.02
CTTATAC26550.035.9548031
GATTACG850.034.823531
TTATACA28250.033.791152
TTACGGG950.033.1052633
TGATACA451.0770418E-732.888892
TATACAC29600.032.4375043
CGTCTTC6150.032.18699337
TAGCAAC352.3845412E-531.7142871
TCAGAGT705.456968E-1231.7142873
CTTCGAT303.5954255E-430.83333232
ATTACTT303.5954255E-430.8333322
TTCGATC303.5954255E-430.83333233
TTTAGTT250.005492219729.59999824
Produced by FastQC (version 0.11.5)