FastQCFastQC Report
Fri 10 Feb 2017
ERR1630961.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630961.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211016
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG127666.049778215869886No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT118475.61426621677977No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT101314.801057739697464RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC64553.0590097433369983No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC38681.8330363574326116RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC34221.6216779770254388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32891.5586495810744208No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30771.458183265723926No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18640.8833453387420859No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG13750.6516093566364636No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA12980.6151192326648216No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12370.5862114721158584No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12220.5791030064070971No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11900.5639382795617394No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT10950.5189179967395837No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9050.42887743109527243No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8780.4160821928195019No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8780.4160821928195019No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8220.38954392084012585No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7870.3729575008530159No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT7830.3710619099973462RNA PCR Primer, Index 26 (95% over 21bp)
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7760.3677446259999242No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7260.3440497403040528No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6390.30282063919323654No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6050.286708116920044No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5720.2710694923607689No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5330.25258748151798915No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5100.2416878340978883No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4980.23600106153087919No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4930.23363157296129203No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4870.23078818667778747No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4850.22984039124995262No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4840.22936649353603517No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4610.2184668461159343No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4350.20614550555408123No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4220.19998483527315464No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4190.19856314213140236No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3980.18861129013913638No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3880.1838723129999621No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3860.18292451757212724No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3810.1805550290025401No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3800.1800811312886227No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT3670.1739204610076961RNA PCR Primer, Index 26 (95% over 23bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3610.17107707472419154No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3460.16396860901543012No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3230.15306896159532926No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3140.1488038821700724No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3080.14596049588656784No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2940.13932592789172385No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2920.138378132463889No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2870.13600864389430187No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2830.13411305303863216No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2830.13411305303863216No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2640.125108996474201No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2630.12463509876028359No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2620.12416120104636615No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2600.1232134056185313No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.12037001933502672No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2540.12037001933502672No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC2530.1198961216211093No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2530.1198961216211093No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2510.11894832619327445No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2490.11800053076543959No Hit
CTGATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT2350.11136596277059559No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2350.11136596277059559No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2350.11136596277059559No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2320.10994426962884331No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA2300.10899647420100846No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2290.10852257648709103No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA2270.10757478105925618No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2270.10757478105925618No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2210.1047313947757516No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2150.10188800849224704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG200.001838532937.09
ACTCAGA200.001838532937.01
TCAGAGT200.001838532937.03
CTCAGAG200.001838532937.02
TTTACAC308.27984E-637.02
TGTATCC308.27984E-637.03
TAAACAT251.2302127E-436.9999962
TCGCCGA251.2302127E-436.9999961
CTTATAC12500.035.6681
CGTCTTC4150.032.98795337
TTATACA13700.032.813872
CTATACA352.379145E-531.7142851
TTACACA352.379145E-531.7142853
GGTATCA3750.031.0800021
TTGTATC303.589614E-430.8333322
GACAGAC303.589614E-430.83333216
GCCGAGC303.589614E-430.8333323
AGACAGA303.589614E-430.83333215
CGCCGAG303.589614E-430.8333322
GTTGTAT303.589614E-430.8333321
Produced by FastQC (version 0.11.5)