##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630961.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	211016
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.858859043863973	31.0	30.0	33.0	28.0	34.0
2	30.85548489214088	31.0	31.0	34.0	27.0	34.0
3	30.285987792394888	31.0	30.0	34.0	26.0	34.0
4	34.47796849527998	35.0	35.0	37.0	30.0	37.0
5	34.67283049626569	35.0	35.0	37.0	32.0	37.0
6	34.889776130719945	35.0	35.0	37.0	32.0	37.0
7	34.87780547446639	35.0	35.0	37.0	32.0	37.0
8	34.923522386928006	35.0	35.0	37.0	32.0	37.0
9	36.658310270311254	38.0	35.0	39.0	32.0	39.0
10	36.479148500587634	38.0	35.0	39.0	32.0	39.0
11	36.50138378132464	38.0	35.0	39.0	32.0	39.0
12	36.383231603290746	38.0	35.0	39.0	32.0	39.0
13	36.51220760511051	38.0	35.0	39.0	32.0	39.0
14	37.20312203813929	39.0	36.0	40.0	32.0	41.0
15	37.222205899078745	39.0	36.0	40.0	32.0	41.0
16	37.186971604048985	39.0	36.0	40.0	32.0	41.0
17	37.09521078970315	39.0	36.0	40.0	31.0	41.0
18	37.34605906661106	39.0	36.0	40.0	32.0	41.0
19	37.302247222959394	39.0	36.0	40.0	32.0	41.0
20	37.40943340789324	39.0	36.0	40.0	32.0	41.0
21	37.350475793304774	39.0	36.0	40.0	32.0	41.0
22	37.03270368123744	39.0	36.0	40.0	31.0	41.0
23	37.17139458619252	39.0	36.0	40.0	31.0	41.0
24	36.790812071122566	38.0	35.0	40.0	31.0	41.0
25	36.831567274519465	38.0	35.0	40.0	31.0	41.0
26	36.67120028812981	38.0	35.0	40.0	30.0	41.0
27	36.3802744815559	38.0	35.0	40.0	30.0	41.0
28	36.56352124957349	38.0	35.0	40.0	30.0	41.0
29	36.4638416044281	38.0	35.0	40.0	30.0	41.0
30	36.23741327671835	38.0	35.0	40.0	30.0	41.0
31	36.09263278613944	38.0	35.0	40.0	30.0	41.0
32	35.81256397619138	38.0	34.0	40.0	30.0	41.0
33	35.628170375706105	38.0	34.0	40.0	29.0	41.0
34	35.48226674754521	38.0	34.0	40.0	28.0	41.0
35	35.45904102058611	38.0	34.0	40.0	28.0	41.0
36	35.325027486067405	38.0	34.0	40.0	27.0	41.0
37	35.26341130530386	38.0	34.0	40.0	27.0	41.0
38	35.14224987678659	38.0	34.0	40.0	26.0	41.0
39	34.8891174128976	38.0	33.0	40.0	26.0	41.0
40	34.44984740493612	37.0	33.0	40.0	24.0	41.0
41	34.354120066724796	37.0	33.0	40.0	24.0	41.0
42	34.10899647420101	37.0	33.0	40.0	23.0	41.0
43	32.95333055313341	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	11.0
11	20.0
12	10.0
13	18.0
14	8.0
15	14.0
16	14.0
17	12.0
18	21.0
19	48.0
20	81.0
21	127.0
22	265.0
23	471.0
24	768.0
25	1228.0
26	1870.0
27	2638.0
28	3755.0
29	4966.0
30	6275.0
31	8103.0
32	9937.0
33	12619.0
34	15932.0
35	20394.0
36	26522.0
37	35508.0
38	41663.0
39	17710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.57328354248019	23.480683929180728	17.99484399287258	24.951188535466503
2	12.583879895363385	21.861375440724874	42.66832846798347	22.886416195928273
3	14.820677105053646	32.0236380179702	35.26509838116541	17.890586495810744
4	9.498805777760928	20.297039087083444	28.36704325738333	41.8371118777723
5	13.530727527770406	38.02886984873185	29.257497061834176	19.18290556166357
6	25.574364029267922	36.827065246237254	15.410205861166926	22.1883648633279
7	21.90070895098002	33.88747772680745	15.898320506501875	28.313492815710656
8	27.80405277324942	28.262785760321492	25.059711111953597	18.87345035447549
9	20.228323918565415	11.264548659817265	14.828733366190242	53.678394055427084
10	15.795484702581794	26.720722599234183	27.591746597414414	29.89204610076961
11	30.50005686772567	19.442127611176403	22.1367100125109	27.921105508587026
12	19.242142775903247	26.966675512757327	21.225404708647684	32.56577700269174
13	36.23469310384046	16.088353489782765	23.917617621412592	23.759335784964172
14	22.921010729044244	25.326989422603024	23.05749327065246	28.694506577700267
15	29.296356674375403	24.505724684384123	18.731281040300264	27.466637600940214
16	28.808242029040454	21.666129582590894	17.53753269894226	31.988095689426395
17	18.95116957955795	33.19226978049058	18.731281040300264	29.12527959965121
18	26.377146756644045	16.179815748568828	23.980172119649694	33.462865375137426
19	24.658319748265534	24.216173181180576	26.678545702695526	24.44696136785836
20	20.08947188838761	18.83885582135952	22.609186033286573	38.462486256966294
21	28.83999317587292	17.31811805739849	26.056791902035865	27.785096864692726
22	20.045873298707207	33.099859726276684	27.332998445615498	19.521268529400615
23	29.96170906471547	19.091917200591425	23.567881108541535	27.378492626151573
24	25.645922584069453	28.373203927664253	27.48891079349433	18.49196269477196
25	25.006634567994844	24.785324335595405	22.402566630018576	27.80547446639117
26	24.486768775827425	21.5249080638435	28.772699700496645	25.215623459832432
27	20.68800470106532	28.194070591803467	28.17985366038594	22.93807104674527
28	18.70569056374872	19.21986958334913	28.35519581453539	33.71924403836676
29	18.530822307313187	22.17699131819388	35.07506539788452	24.21712097660841
30	25.742123819994696	18.28960837092922	30.003885961254124	25.964381847821965
31	22.961765932441143	22.478390264245366	21.851897486446525	32.707946316866966
32	27.52445312203814	20.64961898623801	33.0515221594571	18.774405732266747
33	23.756966296394584	19.73215301209387	31.117071691246164	25.39380900026538
34	17.323330932251583	34.60732835424802	24.949292944610836	23.120047768889563
35	24.31853508738674	19.731205216666034	38.04545626871896	17.904803427228266
36	22.812014254843234	19.916973120521668	23.37832202297456	33.89269060166053
37	21.5874625620806	24.413788527884144	29.810535693975815	24.188213216059445
38	20.472381241232892	17.86546991697312	30.484418243166395	31.17773059862759
39	29.23190658528263	18.844542593926526	25.726959093149333	26.196591727641504
40	18.124691966485955	19.273893922735716	40.85377412139364	21.74763998938469
41	24.54458429692535	21.588410357508437	23.823311976343025	30.043693369223185
42	23.90387458770899	17.850305190127763	36.22000227470903	22.02581794745422
43	25.495223111043714	18.04223376426432	32.314611214315505	24.147931910376464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	4.0
2	7.0
3	38.5
4	70.0
5	70.0
6	131.5
7	193.0
8	219.0
9	245.0
10	371.0
11	497.0
12	497.0
13	802.0
14	1107.0
15	2972.5
16	4838.0
17	4955.0
18	5072.0
19	5072.0
20	4158.5
21	3245.0
22	2557.5
23	1870.0
24	1694.0
25	1518.0
26	1518.0
27	1518.0
28	1518.0
29	1453.0
30	1388.0
31	1429.0
32	1470.0
33	1470.0
34	1695.0
35	1920.0
36	1893.5
37	1867.0
38	2012.0
39	2157.0
40	2157.0
41	2754.5
42	3352.0
43	4705.0
44	6058.0
45	8669.0
46	11280.0
47	11280.0
48	14837.5
49	18395.0
50	19264.0
51	20133.0
52	32533.0
53	44933.0
54	44933.0
55	31748.5
56	18564.0
57	25019.0
58	31474.0
59	23978.0
60	16482.0
61	16482.0
62	10810.0
63	5138.0
64	4004.5
65	2871.0
66	2306.5
67	1742.0
68	1742.0
69	1428.0
70	1114.0
71	749.5
72	385.0
73	219.5
74	54.0
75	54.0
76	40.5
77	27.0
78	22.0
79	17.0
80	12.5
81	8.0
82	8.0
83	6.0
84	4.0
85	2.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	211016.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.058744360617204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.18546883158862	13.94823141373166
2	10.294154213392346	3.9238730712363044
3	4.438421563020613	2.537722258027827
4	2.3920232737399605	1.8235584031542633
5	1.618718452395753	1.5425370588012284
6	1.059253549493995	1.2112825567729462
7	0.8130889922172215	1.084751867156993
8	0.6713578834821096	1.0236190620616445
9	0.5221672427083075	0.895666679303939
>10	3.98587661934008	15.366133373772605
>50	0.5544918815426313	7.326932554877355
>100	0.3953551980505756	14.4605148424764
>500	0.03232463883432379	4.461273078818668
>1k	0.027351617475197057	10.869791864124046
>5k	0.0024865106795633686	3.0590097433369983
>10k+	0.007459532038690106	16.46510217234712
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG	12766	6.049778215869886	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	11847	5.61426621677977	No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	10131	4.801057739697464	RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC	6455	3.0590097433369983	No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC	3868	1.8330363574326116	RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	3422	1.6216779770254388	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3289	1.5586495810744208	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3077	1.458183265723926	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1864	0.8833453387420859	No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG	1375	0.6516093566364636	No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA	1298	0.6151192326648216	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1237	0.5862114721158584	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1222	0.5791030064070971	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1190	0.5639382795617394	No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT	1095	0.5189179967395837	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	905	0.42887743109527243	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	878	0.4160821928195019	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	878	0.4160821928195019	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	822	0.38954392084012585	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	787	0.3729575008530159	No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT	783	0.3710619099973462	RNA PCR Primer, Index 26 (95% over 21bp)
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	776	0.3677446259999242	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	726	0.3440497403040528	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	639	0.30282063919323654	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	605	0.286708116920044	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	572	0.2710694923607689	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	533	0.25258748151798915	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	510	0.2416878340978883	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	498	0.23600106153087919	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	493	0.23363157296129203	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	487	0.23078818667778747	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	485	0.22984039124995262	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	484	0.22936649353603517	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	461	0.2184668461159343	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	435	0.20614550555408123	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	422	0.19998483527315464	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	419	0.19856314213140236	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	398	0.18861129013913638	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	388	0.1838723129999621	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	386	0.18292451757212724	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	381	0.1805550290025401	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	380	0.1800811312886227	No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT	367	0.1739204610076961	RNA PCR Primer, Index 26 (95% over 23bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	361	0.17107707472419154	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	346	0.16396860901543012	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	323	0.15306896159532926	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	314	0.1488038821700724	No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG	308	0.14596049588656784	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	294	0.13932592789172385	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	292	0.138378132463889	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	287	0.13600864389430187	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	283	0.13411305303863216	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	283	0.13411305303863216	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	264	0.125108996474201	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	263	0.12463509876028359	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	262	0.12416120104636615	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	260	0.1232134056185313	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	254	0.12037001933502672	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	254	0.12037001933502672	No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC	253	0.1198961216211093	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	253	0.1198961216211093	No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA	251	0.11894832619327445	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	249	0.11800053076543959	No Hit
CTGATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	235	0.11136596277059559	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	235	0.11136596277059559	No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC	235	0.11136596277059559	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	232	0.10994426962884331	No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA	230	0.10899647420100846	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	229	0.10852257648709103	No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA	227	0.10757478105925618	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA	227	0.10757478105925618	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	221	0.1047313947757516	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	215	0.10188800849224704	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.738977139174281E-4	0.0
8	0.0	0.0	0.0	4.738977139174281E-4	0.0
9	0.0	0.0	0.0	4.738977139174281E-4	0.0
10	0.0	0.0	0.0	4.738977139174281E-4	0.0
11	0.0	0.0	0.0	4.738977139174281E-4	0.0
12	0.0	0.0	0.0	4.738977139174281E-4	0.0
13	0.0	0.0	0.0	9.477954278348562E-4	0.0
14	0.0	0.0	0.0	9.477954278348562E-4	0.0
15	0.0	0.0	0.0	9.477954278348562E-4	0.0
16	0.0	0.0	0.0	9.477954278348562E-4	0.0
17	0.0	0.0	0.0	9.477954278348562E-4	0.0
18	0.0	0.0	0.0	9.477954278348562E-4	0.0
19	0.0	0.0	0.0	0.001421693141752284	0.0
20	0.0	0.0	0.0	0.001421693141752284	0.0
21	0.0	0.0	0.0	0.0023694885695871404	0.0
22	0.0	0.0	0.0	0.004265079425256853	0.0
23	0.0	0.0	0.0	0.0052128748530917085	0.0
24	0.0	0.0	0.0	0.007582363422678849	0.0
25	0.0	0.0	0.0	0.009477954278348561	0.0
26	0.0	0.0	0.0	0.011847442847935702	0.0
27	0.0	0.0	0.0	0.014216931417522841	0.0
28	0.0	0.0	0.0	0.02416878340978883	0.0
29	0.0	0.0	0.0	0.046915873677825376	0.0
30	0.0	0.0	0.0	0.07961481593812791	0.0
31	0.0	0.0	0.0	0.13885203017780642	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAGGG	20	0.0018385329	37.0	9
ACTCAGA	20	0.0018385329	37.0	1
TCAGAGT	20	0.0018385329	37.0	3
CTCAGAG	20	0.0018385329	37.0	2
TTTACAC	30	8.27984E-6	37.0	2
TGTATCC	30	8.27984E-6	37.0	3
TAAACAT	25	1.2302127E-4	36.999996	2
TCGCCGA	25	1.2302127E-4	36.999996	1
CTTATAC	1250	0.0	35.668	1
CGTCTTC	415	0.0	32.987953	37
TTATACA	1370	0.0	32.81387	2
CTATACA	35	2.379145E-5	31.714285	1
TTACACA	35	2.379145E-5	31.714285	3
GGTATCA	375	0.0	31.080002	1
TTGTATC	30	3.589614E-4	30.833332	2
GACAGAC	30	3.589614E-4	30.833332	16
GCCGAGC	30	3.589614E-4	30.833332	3
AGACAGA	30	3.589614E-4	30.833332	15
CGCCGAG	30	3.589614E-4	30.833332	2
GTTGTAT	30	3.589614E-4	30.833332	1
>>END_MODULE