FastQCFastQC Report
Fri 10 Feb 2017
ERR1630960.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630960.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688705
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT135091.9615074669125387No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG132051.9173666519046617No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT108101.5696125336682614TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC68220.9905547367886106No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC38340.5566969892769763RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC31680.4599937563978772No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19700.286044097254993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18490.26847489128146307No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG17590.2554068868383415No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA12300.17859606072266065No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT10990.15957485425545045No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9890.1436028488249686No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT9160.13300324522110338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC17650.033.5410771
CGTCTTC4500.030.01111237
TTACGGG250.00549483429.5999983
TTATACA19950.029.5814532
TATACAC20350.029.090913
CCGTCTT16450.026.99088337
GGTATCA3600.026.7222231
GCCGTCT16700.026.47604836
GTATCAA10250.023.2829271
CGAACTA652.6802536E-622.7692329
AGGACCG652.6802536E-622.769235
TGCGTTA502.7005794E-422.19999937
TGCCGTC20850.021.38369435
AGTTACG450.003824273620.55555716
ATGCCGT21950.020.31207534
GTCTAAG656.896996E-519.9230771
GGACCGT759.256932E-619.7333346
TATGCCG23450.019.09168433
GCGAACT801.6151376E-518.528
CGACACG609.2316914E-418.536
Produced by FastQC (version 0.11.5)