##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630960.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	688705
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.371606130346084	31.0	31.0	34.0	28.0	34.0
2	31.373078458846678	31.0	31.0	34.0	28.0	34.0
3	30.82304179583421	31.0	31.0	34.0	27.0	34.0
4	35.00057208819451	37.0	35.0	37.0	32.0	37.0
5	35.18529413900001	37.0	35.0	37.0	32.0	37.0
6	35.382618102090156	37.0	35.0	37.0	33.0	37.0
7	35.388511772094006	37.0	35.0	37.0	33.0	37.0
8	35.41978786272787	37.0	35.0	37.0	33.0	37.0
9	37.1780123565242	39.0	37.0	39.0	34.0	39.0
10	36.993159625674274	39.0	37.0	39.0	33.0	39.0
11	37.0664217625834	39.0	37.0	39.0	33.0	39.0
12	36.98440406269738	39.0	37.0	39.0	33.0	39.0
13	37.08346389237772	39.0	37.0	39.0	33.0	39.0
14	38.16837833324863	40.0	38.0	41.0	33.0	41.0
15	38.19270660152024	40.0	38.0	41.0	33.0	41.0
16	38.181209661611284	40.0	38.0	41.0	33.0	41.0
17	38.105990228036674	40.0	37.0	41.0	33.0	41.0
18	38.1970829310082	40.0	38.0	41.0	33.0	41.0
19	38.19055038078713	40.0	38.0	41.0	33.0	41.0
20	38.19338613775129	40.0	38.0	41.0	34.0	41.0
21	38.14009917163372	40.0	37.0	41.0	33.0	41.0
22	37.98360836642684	40.0	37.0	41.0	33.0	41.0
23	38.03644521239137	40.0	37.0	41.0	33.0	41.0
24	37.940051255617426	40.0	37.0	41.0	33.0	41.0
25	37.894246448043795	40.0	37.0	41.0	33.0	41.0
26	37.82663549705607	40.0	37.0	41.0	33.0	41.0
27	37.68838036604932	40.0	37.0	41.0	32.0	41.0
28	37.69429291205959	40.0	37.0	41.0	32.0	41.0
29	37.57237133460625	39.0	37.0	41.0	32.0	41.0
30	37.48227615597389	39.0	37.0	41.0	32.0	41.0
31	37.47852128269724	39.0	37.0	41.0	32.0	41.0
32	37.296446228791716	39.0	36.0	41.0	31.0	41.0
33	37.28118134760166	39.0	36.0	41.0	31.0	41.0
34	37.255226838777126	39.0	36.0	41.0	31.0	41.0
35	37.215389753232515	39.0	36.0	41.0	31.0	41.0
36	37.21074770765422	39.0	36.0	41.0	31.0	41.0
37	37.17629173593919	39.0	36.0	40.0	31.0	41.0
38	37.154794868630255	39.0	36.0	40.0	31.0	41.0
39	37.04216173833499	39.0	36.0	40.0	31.0	41.0
40	36.92476604642046	39.0	36.0	40.0	31.0	41.0
41	36.89358433581867	39.0	36.0	40.0	31.0	41.0
42	36.82911696589977	39.0	36.0	40.0	30.0	41.0
43	36.11295983040634	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	12.0
10	16.0
11	12.0
12	18.0
13	11.0
14	10.0
15	6.0
16	10.0
17	10.0
18	18.0
19	46.0
20	98.0
21	174.0
22	309.0
23	622.0
24	1040.0
25	1881.0
26	2983.0
27	4498.0
28	6490.0
29	9067.0
30	12578.0
31	16273.0
32	21520.0
33	27720.0
34	36639.0
35	49260.0
36	68108.0
37	101171.0
38	172098.0
39	155998.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.5651476321502	23.200644688219192	15.613506508592213	24.620701171038398
2	13.586949419562803	21.90458904756028	40.918825912400806	23.58963562047611
3	14.424318104268155	29.019681866691833	34.26009684843293	22.295903180607084
4	11.490841506886113	19.654714282602857	36.98521137497187	31.86923283553916
5	12.46411743779993	38.208086190749306	35.39788443528071	13.929911936170058
6	27.336958494565888	39.79105712895942	17.852926869995137	15.019057506479552
7	23.968752949376	33.69672065688502	22.45417123441822	19.880355159320754
8	24.65511358273862	34.902607066886404	22.575994075837986	17.866285274536995
9	23.33117953260104	14.982902694186917	19.284163756615676	42.401754016596364
10	14.127964803508034	28.681510951713722	33.47746858233932	23.713055662438926
11	31.295111840338024	22.66630850654489	25.08461532876921	20.953964324347872
12	20.15216965173768	26.65030746110454	28.76079017866866	24.43673270848912
13	29.206554330228474	20.389716932503756	26.39736897510545	24.00635976216232
14	21.58732693969116	22.667760507038572	26.014766845020727	29.730145708249538
15	24.112936598398445	28.804059793380333	22.231434358687682	24.851569249533544
16	25.004755301616804	27.146310829745683	23.22736149730291	24.621572371334608
17	20.633798215491396	30.922818913758434	24.536775542503683	23.90660732824649
18	24.229387037991593	25.278747794774247	25.32985821215179	25.162006955082365
19	24.993429697766096	27.28323447629972	25.03858691311955	22.68474891281463
20	22.63291249519025	25.756455957195023	25.074741725412185	26.53588982220254
21	26.53734182269622	25.1155429392846	25.66468952599444	22.68242571202474
22	20.81704067779383	30.664217625833995	27.004595581562498	21.51414611480968
23	24.366310684545635	26.355986961035565	25.603124705062402	23.6745776493564
24	25.776638764057182	27.19959924786374	25.83892958523606	21.184832402843018
25	21.5163241155502	28.138462767077343	27.056577199236248	23.288635918136215
26	26.713905082727727	27.418125322162613	25.462280657175423	20.405688937934237
27	21.012915544391287	28.900472626160695	27.173027638829396	22.913584190618625
28	23.087098249613405	26.41508338112835	27.041185994003236	23.45663237525501
29	24.606471566200334	25.21485977305232	27.888428282065615	22.290240378681727
30	21.162181195141606	27.195243246382706	28.438881669219768	23.203693889255923
31	26.311265345830215	25.757327157491233	25.305755003956705	22.625652492721848
32	21.997807479254543	25.995455238454777	31.03200935088318	20.974727931407497
33	20.567586992979578	29.717222903855788	28.661908945049042	21.053281158115595
34	23.291394719074205	28.273498812989594	26.419729782708124	22.015376685228073
35	21.275292033599293	25.46895985944635	32.624418292302224	20.631329814652137
36	23.183801482492505	27.560711770642005	25.131514944715082	24.12397180215041
37	22.073021104827177	26.539810223535476	28.131638364757045	23.255530306880303
38	21.963249867504956	24.80655723422946	28.261011608743946	24.96918128952164
39	24.422503103651056	25.34858901852027	26.679057070879402	23.549850806949273
40	21.02656434903188	24.632462375037207	32.35013539904604	21.99083787688488
41	22.514429254905945	26.003876841318124	26.0439520549437	25.43774184883223
42	22.660355304520806	24.754866016654447	30.17460305936504	22.41017561945971
43	23.216616693649677	24.278319454628612	29.510748433654467	22.99431541806724
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	9.5
2	17.0
3	55.0
4	93.0
5	93.0
6	130.0
7	167.0
8	171.5
9	176.0
10	261.5
11	347.0
12	347.0
13	561.0
14	775.0
15	1891.0
16	3007.0
17	3504.5
18	4002.0
19	4002.0
20	4084.0
21	4166.0
22	5063.0
23	5960.0
24	7804.5
25	9649.0
26	9649.0
27	12286.5
28	14924.0
29	17811.0
30	20698.0
31	24072.5
32	27447.0
33	27447.0
34	31083.0
35	34719.0
36	37464.5
37	40210.0
38	43317.5
39	46425.0
40	46425.0
41	47927.5
42	49430.0
43	50300.0
44	51170.0
45	51424.0
46	51678.0
47	51678.0
48	51775.0
49	51872.0
50	63038.5
51	74205.0
52	58677.0
53	43149.0
54	43149.0
55	49512.0
56	55875.0
57	46504.0
58	37133.0
59	28973.0
60	20813.0
61	20813.0
62	17847.5
63	14882.0
64	12522.5
65	10163.0
66	8348.5
67	6534.0
68	6534.0
69	5295.5
70	4057.0
71	3153.5
72	2250.0
73	1783.0
74	1316.0
75	1316.0
76	1002.0
77	688.0
78	520.5
79	353.0
80	276.0
81	199.0
82	199.0
83	143.5
84	88.0
85	67.5
86	47.0
87	29.5
88	12.0
89	12.0
90	9.0
91	6.0
92	3.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	688705.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.98546407537942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.20372488068509	45.20009961289283
2	10.483680559469748	11.52900081560955
3	2.805134437198495	4.627248564695625
4	1.2549877356444221	2.7602433221327254
5	0.6889272292294195	1.8940491706672464
6	0.48284401592310094	1.5929641374930956
7	0.3241640153680736	1.2477016175086368
8	0.24135659610072696	1.0616883555401906
9	0.18370633305246306	0.9091060178828304
>10	1.1871260664353118	12.613023867384044
>50	0.10019772522989383	3.7378271160033822
>100	0.03965605299093235	3.598707889809951
>500	0.0015862421196337392	0.6146120923450747
>1k	0.0018506158062393623	2.167278071138297
>5k	2.6437368660562325E-4	0.991694345784792
>10k+	7.931210598168696E-4	5.454755003111775
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	13509	1.9615074669125387	No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	13205	1.9173666519046617	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	10810	1.5696125336682614	TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	6822	0.9905547367886106	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	3834	0.5566969892769763	RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	3168	0.4599937563978772	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1970	0.286044097254993	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1849	0.26847489128146307	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	1759	0.2554068868383415	No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	1230	0.17859606072266065	No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT	1099	0.15957485425545045	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	989	0.1436028488249686	No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	916	0.13300324522110338	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.452000493680168E-4	0.0
5	0.0	0.0	0.0	1.452000493680168E-4	0.0
6	0.0	0.0	0.0	1.452000493680168E-4	0.0
7	0.0	0.0	0.0	1.452000493680168E-4	0.0
8	0.0	0.0	0.0	1.452000493680168E-4	0.0
9	0.0	0.0	0.0	1.452000493680168E-4	0.0
10	0.0	0.0	0.0	1.452000493680168E-4	0.0
11	0.0	0.0	0.0	1.452000493680168E-4	0.0
12	0.0	0.0	0.0	1.452000493680168E-4	0.0
13	0.0	0.0	0.0	2.904000987360336E-4	0.0
14	0.0	0.0	0.0	2.904000987360336E-4	0.0
15	0.0	0.0	0.0	2.904000987360336E-4	0.0
16	0.0	0.0	0.0	2.904000987360336E-4	1.452000493680168E-4
17	0.0	0.0	0.0	2.904000987360336E-4	1.452000493680168E-4
18	0.0	0.0	0.0	2.904000987360336E-4	1.452000493680168E-4
19	0.0	0.0	0.0	4.3560014810405036E-4	1.452000493680168E-4
20	0.0	0.0	0.0	5.808001974720671E-4	1.452000493680168E-4
21	0.0	0.0	0.0	0.0010164003455761174	1.452000493680168E-4
22	0.0	0.0	0.0	0.0014520004936801679	1.452000493680168E-4
23	0.0	0.0	0.0	0.0018876006417842181	1.452000493680168E-4
24	0.0	0.0	0.0	0.002758800937992319	1.452000493680168E-4
25	0.0	0.0	0.0	0.0029040009873603357	1.452000493680168E-4
26	0.0	0.0	0.0	0.0030492010367283526	2.904000987360336E-4
27	0.0	0.0	0.0	0.0037752012835684363	2.904000987360336E-4
28	0.0	0.0	0.0	0.007114802419032822	2.904000987360336E-4
29	0.0	0.0	0.0	0.015536405282377796	2.904000987360336E-4
30	0.0	0.0	0.0	0.028604409725499307	2.904000987360336E-4
31	0.0	0.0	0.0	0.05938682019151886	2.904000987360336E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1765	0.0	33.541077	1
CGTCTTC	450	0.0	30.011112	37
TTACGGG	25	0.005494834	29.599998	3
TTATACA	1995	0.0	29.581453	2
TATACAC	2035	0.0	29.09091	3
CCGTCTT	1645	0.0	26.990883	37
GGTATCA	360	0.0	26.722223	1
GCCGTCT	1670	0.0	26.476048	36
GTATCAA	1025	0.0	23.282927	1
CGAACTA	65	2.6802536E-6	22.76923	29
AGGACCG	65	2.6802536E-6	22.76923	5
TGCGTTA	50	2.7005794E-4	22.199999	37
TGCCGTC	2085	0.0	21.383694	35
AGTTACG	45	0.0038242736	20.555557	16
ATGCCGT	2195	0.0	20.312075	34
GTCTAAG	65	6.896996E-5	19.923077	1
GGACCGT	75	9.256932E-6	19.733334	6
TATGCCG	2345	0.0	19.091684	33
GCGAACT	80	1.6151376E-5	18.5	28
CGACACG	60	9.2316914E-4	18.5	36
>>END_MODULE