FastQCFastQC Report
Fri 10 Feb 2017
ERR1630959.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630959.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322038
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT219216.806960669237792No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG166385.1664710375794165No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT150994.688577124438731RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC85592.6577608853613546No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC59861.8587868512411578RNA PCR Primer, Index 17 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48071.492680987957943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46441.442065843161366No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC46251.4361659183077773No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA31080.9651034971028264No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG19020.590613530080301No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18480.5738453226016805No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA18450.5729137555195349No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA17890.555524503319484No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16600.515467118787224No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT15280.4744781671728181No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14590.4530521242834697No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13270.4120631726690639No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT13100.4067842925369056No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12580.3906371297797155No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG12490.3878424285332787No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC12080.3751110117439557No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11280.35026922288674006No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT11020.342195641508145No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8720.27077549854365013No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8480.2633229618864854No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7920.24593370968643452No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7750.24065482955427622No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7530.2338233376185419No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7390.2294760245685292No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG7320.2273023680435228No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG7190.22326557735422528No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7140.2217129655506493No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7090.22016035374707332No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7060.21922878666492773No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6470.20090796738273126No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6420.19935535557915524No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6120.1900396847576994No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5840.18134505865767392No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5710.1773082679683764No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5670.17606617852551562No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5590.17358199963979407No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5370.16675050770405977No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5350.16612946298262937No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5030.15619274743974312No Hit
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT4960.15401909091473678No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4950.1537085685540216No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4690.1456349871754265No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4650.14439289773256572No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4560.14159819648612895No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4520.14035610704326817No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4490.13942453996112258No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4390.13631931635397065No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4370.13569827163254025No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4350.13507722691110988No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4260.1322825256646731No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4250.13197200330395792No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4230.13135095858252752No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG4190.13010886913966674No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4160.12917730205752118No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT4140.12855625733609077No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.12824573497537559No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4040.12545103372893882No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4020.12482998900750844No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4000.12420894428607804No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3840.11924058651463493No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3800.11799849707177415No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3660.1136511840217614No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3630.11271961693961581No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3600.11178804985747023No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3520.10930387097174868No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3480.1080617815288879No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3470.10775125916817269No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3430.10650916972531192No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3410.10588812500388152No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3330.10340394611815996No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA3240.10060924487172321No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACA200.00183996937.03
ATTACGG551.2732926E-1137.02
TTACGGG551.2732926E-1137.03
CTTATAC23550.035.114651
GATTACG603.45608E-1133.9166681
TTATACA24550.033.684322
CTTGAAA507.2977855E-933.30000327
CGTCTTC6850.032.67883337
GACAGAC401.5953356E-632.37516
ACAGACA401.5953356E-632.37517
CAGACAC401.5953356E-632.37518
TATACAC25800.032.267443
TCAGAGT750.032.0666663
ACTCAGA750.032.0666661
CTCAGAG750.032.0666662
CTGCTTG705.456968E-1231.71428724
TTACTTT352.3830104E-531.7142873
GACCGTT658.731149E-1131.3076927
TAGGACC658.731149E-1131.3076924
TTTACAC502.7104943E-729.62
Produced by FastQC (version 0.11.5)