FastQCFastQC Report
Fri 10 Feb 2017
ERR1630958.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630958.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561527
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT247024.399076090731167No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG195633.483893027405628No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT157462.8041394269554267TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC103081.8357087014515776No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC59711.0633504711260546RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC48230.8589079420936125TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41280.7351382925487109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40010.7125213925599303No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA27110.4827906761384582No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG23970.4268717265598983No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA19760.351897593526224No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16160.2877866959202318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15740.2803070911995327No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT15130.26944385577185065No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT14430.25697784790401884No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12380.22047025343393994No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT12250.21815513768705688No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11620.20693573060600826No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11300.20123698415214228No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11110.19785335344515934No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10710.19072992037782688No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10520.18734628967084396No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG9840.17523645345637878No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8710.15511275504116454No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7690.13694800071946672No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7640.1360575715860502No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7480.1332081983591172No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7110.12661902277183465No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6900.12287922041148511No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6760.12038601883791875No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6710.11949558970450219No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6490.11557770151746932No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6450.1148653582107361No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6410.11415301490400283No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6260.11148172750375315No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6160.10970086923692005No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6150.10952278341023673No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6090.10845426845013685No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6080.10827618262345354No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6050.10774192514340361No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5970.10631723852993712No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5850.10418020860973737No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5800.10328977947632083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGTG200.001841134637.06
TCTGTGC452.6157068E-937.024
CTCTCTG452.6157068E-937.021
GATTACG900.037.01
CTGTGCT452.6157068E-937.025
CTTATAC27950.034.3524131
TTATACA28700.033.4547042
AACCACG507.3177944E-933.30000335
CTCTGTG507.3177944E-933.30000323
TTACGGG1150.032.1739123
CGTCTTC7950.032.1132137
GATACAC352.3861488E-531.7142853
TATACAC30500.031.5409833
TGTGCTA551.8522769E-830.27272826
TGCTAGT551.8522769E-830.27272828
CCACGTT551.8522769E-830.27272837
GTAACCA551.8522769E-830.27272833
AGTAACC551.8522769E-830.27272832
GGTATCA5350.030.0841141
CTTAATA250.005493978529.61
Produced by FastQC (version 0.11.5)