##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630958.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	561527
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.385238821997874	31.0	31.0	34.0	28.0	34.0
2	31.400258580620346	31.0	31.0	34.0	28.0	34.0
3	30.87772449054097	31.0	31.0	34.0	27.0	34.0
4	35.01520674874049	37.0	35.0	37.0	32.0	37.0
5	35.2146521894762	37.0	35.0	37.0	32.0	37.0
6	35.41916061026451	37.0	35.0	37.0	33.0	37.0
7	35.41130702530778	37.0	35.0	37.0	33.0	37.0
8	35.43818551912909	37.0	35.0	37.0	33.0	37.0
9	37.232109943065964	39.0	37.0	39.0	34.0	39.0
10	37.048058241188755	39.0	37.0	39.0	33.0	39.0
11	37.10444911820803	39.0	37.0	39.0	33.0	39.0
12	37.01526729792156	39.0	37.0	39.0	33.0	39.0
13	37.11821159089411	39.0	37.0	39.0	33.0	39.0
14	38.105654759254676	40.0	37.0	41.0	33.0	41.0
15	38.10860564140282	40.0	37.0	41.0	33.0	41.0
16	38.12815590345611	40.0	37.0	41.0	33.0	41.0
17	38.08526393209944	40.0	37.0	41.0	33.0	41.0
18	38.2043801989931	40.0	38.0	41.0	33.0	41.0
19	38.16768027183021	40.0	37.0	41.0	33.0	41.0
20	38.105941477435636	40.0	37.0	41.0	33.0	41.0
21	38.040603568483796	40.0	37.0	41.0	33.0	41.0
22	37.89147449721919	40.0	37.0	41.0	33.0	41.0
23	37.96601588169403	40.0	37.0	41.0	33.0	41.0
24	37.89205149529764	40.0	37.0	41.0	33.0	41.0
25	37.80338078133376	40.0	37.0	41.0	33.0	41.0
26	37.71040929465546	39.0	37.0	41.0	32.0	41.0
27	37.53764823418999	39.0	37.0	41.0	32.0	41.0
28	37.57685917150912	39.0	37.0	41.0	32.0	41.0
29	37.4789173094081	39.0	37.0	41.0	32.0	41.0
30	37.28753381404634	39.0	36.0	41.0	31.0	41.0
31	37.24081121655771	39.0	36.0	41.0	31.0	41.0
32	37.08020629462163	39.0	36.0	40.0	31.0	41.0
33	37.06678752758104	39.0	36.0	40.0	31.0	41.0
34	37.08065685176314	39.0	36.0	40.0	31.0	41.0
35	37.02833345502531	39.0	35.0	40.0	31.0	41.0
36	37.0	39.0	35.0	41.0	31.0	41.0
37	36.931317639223046	39.0	35.0	40.0	31.0	41.0
38	36.84496382186431	39.0	35.0	40.0	31.0	41.0
39	36.703533400887224	39.0	35.0	40.0	30.0	41.0
40	36.482110388280525	39.0	35.0	40.0	30.0	41.0
41	36.453664739184404	39.0	35.0	40.0	30.0	41.0
42	36.324180315461234	39.0	35.0	40.0	30.0	41.0
43	35.46818051491736	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	12.0
10	15.0
11	18.0
12	25.0
13	11.0
14	16.0
15	11.0
16	17.0
17	18.0
18	22.0
19	43.0
20	83.0
21	145.0
22	312.0
23	545.0
24	1002.0
25	1690.0
26	2637.0
27	4153.0
28	5951.0
29	8210.0
30	11297.0
31	14560.0
32	18580.0
33	23741.0
34	31010.0
35	41580.0
36	56210.0
37	81873.0
38	130778.0
39	126961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.39313336669475	22.781451292635975	15.888995542511758	25.936419798157523
2	13.302833167416706	20.7728212534749	42.4836205560908	23.440725023017592
3	13.493919259447898	30.6800919635209	34.57447282143156	21.251515955599643
4	9.968354148598376	20.77442402591505	34.46495003802133	34.79227178746525
5	12.375006010396651	37.84252582689702	34.3445640191834	15.437904143522928
6	26.00053069576352	39.97830914630997	16.458335930418304	17.562824227508205
7	23.451588258445273	32.7706414829563	20.126013530961114	23.65175672763732
8	25.089977863931743	32.55177400196251	23.14634915151008	19.21189898259567
9	22.279961604695767	12.950045144757064	17.320805589045584	47.44918766150158
10	15.45304143879101	27.562699567429526	30.84036920753588	26.143889786243584
11	30.38589417784007	21.885679584418916	24.36445620602393	23.363970031717084
12	19.980517410560847	27.491465236756202	23.813992915745814	28.714024436937137
13	31.2350073994661	18.463048081392348	25.913090554862013	24.388853964279544
14	22.358141282609743	23.09577277673202	23.857267771629857	30.68881816902838
15	26.389113969586504	26.598364815939394	20.79490389598363	26.217617318490472
16	27.297351685671394	24.121012880947845	20.888220869165686	27.69341456421508
17	20.091821052237915	32.09355916990634	21.246173380799142	26.5684463970566
18	26.553131015961835	21.5913037129114	23.383381386825565	28.4721838843012
19	28.457224674859805	25.10012875605269	23.264598140427797	23.178048428659707
20	24.49552737446285	23.21206282155622	23.96874949913361	28.32366030484732
21	27.452998698192605	25.38595650787941	23.43787564979066	23.723169144137326
22	23.476342188354256	31.936309384944984	23.537781798559998	21.049566628140767
23	24.09643703686555	22.599803749418996	25.51560298970486	27.788156224010603
24	26.990331720469364	27.515684909185133	25.29228336304399	20.20170000730152
25	22.05895709378178	30.683475594227883	23.660126761491433	23.59744055049891
26	28.26774135526876	25.834910876948037	24.181918233673535	21.715429534109667
27	25.262008772507823	30.02794166620661	24.53524051381323	20.17480904747234
28	18.754574579672926	26.13569783821615	27.873815506645272	27.235912075465652
29	29.71415443959062	23.10930729955995	25.479985824368196	21.696552436481237
30	24.246385302932897	24.29553699109749	27.47365665408787	23.984421051881743
31	22.695435838347933	25.79110176358394	26.786245363090288	24.727217034977837
32	25.234939726851955	27.026661229112758	26.64769458993067	21.09070445410461
33	18.54069350182627	22.738888780058662	29.78200513955696	28.938412578558108
34	19.363628106929852	27.81023886651933	30.523198350212898	22.302934676337916
35	19.940626185383785	27.672934694146495	32.701009924723124	19.685429195746597
36	23.493438427715855	23.34705187818217	22.775752546182108	30.38375714791987
37	21.365134713023597	26.169712231112662	28.39240143394708	24.072751621916666
38	21.28054394534902	22.002325800896482	29.531794553066902	27.18533570068759
39	25.61996128414128	22.906111371314292	26.136054009869515	25.337873334674914
40	19.484726469074506	22.750820530446443	36.58808926373977	21.176363736739283
41	23.06799138776942	23.56057678437546	24.726504691671103	28.644927136184013
42	23.230049490051236	22.613160186420245	31.729017482685606	22.427772840842916
43	24.12190331008126	21.212515159556	31.124059929442392	23.541521600920348
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	12.5
2	13.0
3	75.0
4	137.0
5	137.0
6	196.0
7	255.0
8	291.5
9	328.0
10	497.0
11	666.0
12	666.0
13	1091.5
14	1517.0
15	3756.5
16	5996.0
17	6233.5
18	6471.0
19	6471.0
20	5258.0
21	4045.0
22	3163.5
23	2282.0
24	2443.0
25	2604.0
26	2604.0
27	3309.5
28	4015.0
29	5187.5
30	6360.0
31	8648.0
32	10936.0
33	10936.0
34	14467.0
35	17998.0
36	20013.5
37	22029.0
38	25742.5
39	29456.0
40	29456.0
41	31824.0
42	34192.0
43	37321.0
44	40450.0
45	43152.5
46	45855.0
47	45855.0
48	47314.0
49	48773.0
50	48582.0
51	48391.0
52	67130.5
53	85870.0
54	85870.0
55	62730.0
56	39590.0
57	46174.5
58	52759.0
59	40821.5
60	28884.0
61	28884.0
62	19641.5
63	10399.0
64	7880.0
65	5361.0
66	4240.0
67	3119.0
68	3119.0
69	2344.5
70	1570.0
71	1136.5
72	703.0
73	445.5
74	188.0
75	188.0
76	141.0
77	94.0
78	84.5
79	75.0
80	54.0
81	33.0
82	33.0
83	30.0
84	27.0
85	35.5
86	44.0
87	31.5
88	19.0
89	19.0
90	15.0
91	11.0
92	5.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	561527.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.369471102903333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.81731742077264	11.101336177957604
2	10.558208857798714	3.4566458959230815
3	4.698702118169257	2.307458056335671
4	2.8231377625953282	1.8485308809727758
5	1.938663388418064	1.5867447157483079
6	1.3849149794928144	1.3602195442071352
7	1.06833190091276	1.224161972621085
8	0.9171118049587137	1.2010108151522545
9	0.7082322479574408	1.0434048585375235
>10	6.433925521382957	21.860035225376517
>50	0.9562767218964523	11.049869374046128
>100	0.6440453007539246	19.372710484090703
>500	0.027197858984540735	2.916333497765913
>1k	0.018494544109487702	6.085370783595446
>5k	0.0010879143593816294	1.0633504711260546
>10k+	0.004351657437526518	12.522817246543799
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	24702	4.399076090731167	No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG	19563	3.483893027405628	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	15746	2.8041394269554267	TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC	10308	1.8357087014515776	No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC	5971	1.0633504711260546	RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	4823	0.8589079420936125	TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4128	0.7351382925487109	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4001	0.7125213925599303	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2711	0.4827906761384582	No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG	2397	0.4268717265598983	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA	1976	0.351897593526224	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1616	0.2877866959202318	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1574	0.2803070911995327	No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT	1513	0.26944385577185065	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1443	0.25697784790401884	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1238	0.22047025343393994	No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT	1225	0.21815513768705688	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1162	0.20693573060600826	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	1130	0.20123698415214228	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1111	0.19785335344515934	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	1071	0.19072992037782688	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	1052	0.18734628967084396	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	984	0.17523645345637878	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	871	0.15511275504116454	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	769	0.13694800071946672	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	764	0.1360575715860502	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	748	0.1332081983591172	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	711	0.12661902277183465	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	690	0.12287922041148511	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	676	0.12038601883791875	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	671	0.11949558970450219	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	649	0.11557770151746932	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	645	0.1148653582107361	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	641	0.11415301490400283	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	626	0.11148172750375315	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	616	0.10970086923692005	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	615	0.10952278341023673	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	609	0.10845426845013685	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	608	0.10827618262345354	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	605	0.10774192514340361	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	597	0.10631723852993712	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	585	0.10418020860973737	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	580	0.10328977947632083	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.7808582668331176E-4	0.0
13	0.0	0.0	0.0	1.7808582668331176E-4	0.0
14	0.0	0.0	0.0	1.7808582668331176E-4	0.0
15	0.0	0.0	0.0	1.7808582668331176E-4	0.0
16	0.0	0.0	0.0	1.7808582668331176E-4	0.0
17	0.0	0.0	0.0	3.561716533666235E-4	0.0
18	0.0	0.0	0.0	3.561716533666235E-4	0.0
19	0.0	0.0	0.0	5.342574800499352E-4	0.0
20	0.0	0.0	0.0	7.12343306733247E-4	0.0
21	0.0	0.0	0.0	0.0017808582668331176	0.0
22	0.0	0.0	0.0	0.0030274590536163	0.0
23	0.0	0.0	0.0	0.004452145667082794	0.0
24	0.0	0.0	0.0	0.006233003933915912	0.0
25	0.0	0.0	0.0	0.006589175587282535	0.0
26	0.0	0.0	0.0	0.006945347240649159	0.0
27	0.0	0.0	0.0	0.00819194802743234	0.0
28	0.0	0.0	0.0	0.01798666849501449	0.0
29	0.0	0.0	0.0	0.04345294171072807	0.0
30	0.0	0.0	0.0	0.07034390153990815	0.0
31	0.0	0.0	0.0	0.12501625033168484	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAGTG	20	0.0018411346	37.0	6
TCTGTGC	45	2.6157068E-9	37.0	24
CTCTCTG	45	2.6157068E-9	37.0	21
GATTACG	90	0.0	37.0	1
CTGTGCT	45	2.6157068E-9	37.0	25
CTTATAC	2795	0.0	34.352413	1
TTATACA	2870	0.0	33.454704	2
AACCACG	50	7.3177944E-9	33.300003	35
CTCTGTG	50	7.3177944E-9	33.300003	23
TTACGGG	115	0.0	32.173912	3
CGTCTTC	795	0.0	32.11321	37
GATACAC	35	2.3861488E-5	31.714285	3
TATACAC	3050	0.0	31.540983	3
TGTGCTA	55	1.8522769E-8	30.272728	26
TGCTAGT	55	1.8522769E-8	30.272728	28
CCACGTT	55	1.8522769E-8	30.272728	37
GTAACCA	55	1.8522769E-8	30.272728	33
AGTAACC	55	1.8522769E-8	30.272728	32
GGTATCA	535	0.0	30.084114	1
CTTAATA	25	0.0054939785	29.6	1
>>END_MODULE