FastQCFastQC Report
Fri 10 Feb 2017
ERR1630957.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630957.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360703
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG157974.379503358718946No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT146724.067612412427954No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT132423.671164365142513TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC81932.2713977981885374No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC52411.4529959551209721RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC38541.0684690728937658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35000.9703273884608667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32480.9004638164916843No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG18040.500134459652401No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17340.4807279118831837No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA15500.4297164148898124No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT13380.3709422987887542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13050.3617934976975517No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11360.314940546654727No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10220.28333559743057307No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT9710.2691965411987147No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8830.2447997382888415No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7550.20931347951084409No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7350.203768751576782No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7270.20155086040315717No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6800.18852074975811126No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6610.18325325822075225No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6210.17216380235262807No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5580.15469790936033248No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4890.13556859798781823No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4870.13501412519441203No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4620.1280832152768344No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4590.12725150608672509No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4560.12641979689661578No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.12531085130980335No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4490.12447914211969405No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4310.11948888697903814No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT4280.11865717778892883No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4250.11782546859881951No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4140.11477586823508538No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4100.11366692264827295No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4080.11311244985486675No Hit
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT3970.11006284949113258TruSeq Adapter, Index 11 (95% over 23bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3960.10978561309442948No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3750.10396364876366429No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3730.10340917597025807No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3690.10230023038344566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTT200.001840262437.029
GGTCGCT200.001840262437.028
GCTCTAG200.001840262437.01
TCTCGCG200.001840262437.020
AGTTCCT200.001840262437.05
CTCTCGC200.001840262437.019
GATTACG403.8207872E-837.01
CTTATAC17200.034.526161
TTATACA17600.033.7414782
CGTCTTC7750.032.22580737
TGATACA352.3838002E-531.7142852
AGACAGA352.3838002E-531.71428515
ACAGACA352.3838002E-531.71428517
CAGACAC352.3838002E-531.71428518
TATACAC19150.031.2036533
TCAGAGT303.594627E-430.8333323
AACTAGA303.594627E-430.83333227
TAACTAG303.594627E-430.83333226
TGATCAC250.005491405329.626
ACTGCCA250.005491405329.611
Produced by FastQC (version 0.11.5)