##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630957.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	360703
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.965220694033597	31.0	30.0	34.0	28.0	34.0
2	30.95460254003987	31.0	31.0	34.0	27.0	34.0
3	30.380418238828067	31.0	30.0	34.0	26.0	34.0
4	34.5830558659063	35.0	35.0	37.0	31.0	37.0
5	34.76224483855138	35.0	35.0	37.0	32.0	37.0
6	34.984505257788264	35.0	35.0	37.0	32.0	37.0
7	34.986093822341374	35.0	35.0	37.0	32.0	37.0
8	35.03408344261068	36.0	35.0	37.0	32.0	37.0
9	36.776009625647696	39.0	37.0	39.0	32.0	39.0
10	36.562301949249104	38.0	35.0	39.0	32.0	39.0
11	36.601971705253355	38.0	35.0	39.0	32.0	39.0
12	36.50660792951542	38.0	35.0	39.0	32.0	39.0
13	36.6403883527445	38.0	35.0	39.0	32.0	39.0
14	37.46075580186469	39.0	36.0	40.0	32.0	41.0
15	37.48794714765333	39.0	36.0	40.0	32.0	41.0
16	37.451421252387696	39.0	36.0	40.0	32.0	41.0
17	37.4448452050579	39.0	36.0	40.0	32.0	41.0
18	37.53862596097066	39.0	36.0	40.0	32.0	41.0
19	37.50538808936992	39.0	36.0	40.0	32.0	41.0
20	37.51086628056878	39.0	37.0	40.0	32.0	41.0
21	37.37984713185086	39.0	36.0	40.0	32.0	41.0
22	37.24489677102769	39.0	36.0	40.0	32.0	41.0
23	37.33425560641303	39.0	36.0	40.0	32.0	41.0
24	37.22632193244858	39.0	36.0	40.0	31.0	41.0
25	37.15439849405189	39.0	36.0	40.0	31.0	41.0
26	36.91462227927131	39.0	36.0	40.0	31.0	41.0
27	36.78961638799788	39.0	35.0	40.0	31.0	41.0
28	36.70839721321974	39.0	35.0	40.0	30.0	41.0
29	36.64338527819286	38.0	35.0	40.0	30.0	41.0
30	36.52862604414158	38.0	35.0	40.0	30.0	41.0
31	36.38183491681522	38.0	35.0	40.0	30.0	41.0
32	36.208082549909484	38.0	35.0	40.0	30.0	41.0
33	36.19263771024915	38.0	35.0	40.0	30.0	41.0
34	36.11384712630613	38.0	35.0	40.0	30.0	41.0
35	36.07525581988506	38.0	35.0	40.0	30.0	41.0
36	36.03383392985365	38.0	35.0	40.0	30.0	41.0
37	35.917863172748774	38.0	35.0	40.0	29.0	41.0
38	35.789244891226296	38.0	34.0	40.0	28.0	41.0
39	35.62322187506064	38.0	34.0	40.0	27.0	41.0
40	35.34634588567325	38.0	34.0	40.0	26.0	41.0
41	35.315239961963165	38.0	34.0	40.0	26.0	41.0
42	35.18030900768777	38.0	34.0	40.0	26.0	41.0
43	34.21875060645462	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	5.0
10	13.0
11	20.0
12	13.0
13	9.0
14	17.0
15	6.0
16	9.0
17	14.0
18	31.0
19	39.0
20	76.0
21	197.0
22	334.0
23	615.0
24	1074.0
25	1653.0
26	2671.0
27	3950.0
28	5450.0
29	7219.0
30	9462.0
31	12084.0
32	15149.0
33	19414.0
34	25052.0
35	32196.0
36	43822.0
37	61189.0
38	78767.0
39	40145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.886604769020494	23.843716298450527	17.28541209804188	24.984266834487098
2	13.402716362214898	21.221614458432562	41.70772075641179	23.667948422940757
3	14.06198451357488	31.35543646712115	34.406145776442116	20.176433242861854
4	10.09556338594356	20.34721086323097	32.41253884775009	37.14468690307539
5	12.429893846183703	38.475144370853585	32.68672564408946	16.408236138873256
6	25.769677546346998	39.029894400656495	16.348353077185386	18.852074975811124
7	22.791881409358947	33.60188298960641	19.306742666404215	24.29949293463043
8	26.143392209102778	30.998633224564255	23.797140583804406	19.060833982528564
9	21.36882698508191	12.411596244001299	16.632243147409365	49.587333623507426
10	15.226931852521327	26.820126253455058	30.449705159092105	27.50323673493151
11	29.839785086345277	21.453938558869762	23.440337341247506	25.26593901353745
12	19.274306007989953	28.084046986024514	23.172804218429015	29.46884278755652
13	33.15109660856716	17.438446589021993	24.77107204542241	24.639384756988434
14	22.81988228542596	24.089070509532775	23.570083974904563	29.520963230136704
15	27.188850661070184	26.77465948439575	19.801332398122558	26.235157456411507
16	27.455829311095275	23.376572970005792	20.143996584447592	29.023601134451336
17	19.418468934275566	32.61963443608731	20.819898919609763	27.141997710027365
18	24.972622905825567	19.905573283282923	23.038067329631303	32.0837364812602
19	25.24958206613197	25.23516577350341	23.202191276479542	26.313060883885083
20	24.506034050174243	21.86258500761014	25.03555556787717	28.595825374338446
21	26.237375347585136	22.654372156594206	27.02666736899887	24.08158512682179
22	19.775272176832466	35.929559776325675	23.290906923424533	21.004261123417326
23	23.948234420007598	20.457828185515506	26.55481102181019	29.039126372666708
24	22.88253771108086	28.54287322256815	26.892484952994565	21.682104113356417
25	22.91719226066875	30.049375802252825	24.148399098427237	22.88503283865119
26	26.88361338830007	23.381008752353043	26.685943837450754	23.049434021896133
27	23.281480885936627	32.373171279418244	25.01614902010796	19.32919881453717
28	18.418754487764172	21.31532036051821	33.41862973138566	26.847295420331964
29	20.009259695649884	26.68344870988043	26.806541670016603	26.500749924453082
30	23.01339329032473	23.799912947771436	28.782128232922933	24.404565528980907
31	24.226856998694217	20.44646149325068	32.312456508540265	23.014224999514838
32	20.6003276934209	32.217641660867805	27.056608899842804	20.125421745868486
33	22.590607785352493	22.457534314935003	30.618542124684296	24.333315775028208
34	18.876194542324296	28.791277034014133	30.38316842388336	21.949359999778213
35	20.15203643995198	26.51294832590802	34.93733071252526	18.397684521614735
36	27.79904797021372	22.445335913480065	23.1001682824928	26.65544783381342
37	21.436749902274173	24.942126902188228	29.34353193624672	24.277591259290883
38	21.011192033334904	21.12513619237988	29.436406129142256	28.427265645142956
39	26.89442560777149	22.079383869831968	26.28006975267741	24.74612076971913
40	18.915562110656136	21.344984654965444	37.99857500492095	21.740878229457476
41	23.167813963288356	23.628580854608916	24.77356717299274	28.430038009109985
42	22.59310291292282	21.27068530064901	33.927358519335854	22.20885326709232
43	24.26539285783595	20.521038083963816	31.56170034626826	23.65186871193198
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	9.0
2	10.0
3	54.0
4	98.0
5	98.0
6	153.5
7	209.0
8	258.5
9	308.0
10	434.0
11	560.0
12	560.0
13	923.5
14	1287.0
15	3256.0
16	5225.0
17	5314.0
18	5403.0
19	5403.0
20	4389.5
21	3376.0
22	2705.0
23	2034.0
24	2106.0
25	2178.0
26	2178.0
27	2559.0
28	2940.0
29	3604.5
30	4269.0
31	5282.5
32	6296.0
33	6296.0
34	7945.5
35	9595.0
36	10683.5
37	11772.0
38	13510.0
39	15248.0
40	15248.0
41	16939.0
42	18630.0
43	20731.5
44	22833.0
45	24846.0
46	26859.0
47	26859.0
48	30879.0
49	34899.0
50	47160.5
51	59422.0
52	43834.0
53	28246.0
54	28246.0
55	37799.5
56	47353.0
57	37611.5
58	27870.0
59	18789.0
60	9708.0
61	9708.0
62	8109.0
63	6510.0
64	4963.5
65	3417.0
66	2715.0
67	2013.0
68	2013.0
69	1574.5
70	1136.0
71	865.0
72	594.0
73	383.0
74	172.0
75	172.0
76	122.0
77	72.0
78	58.5
79	45.0
80	36.0
81	27.0
82	27.0
83	23.5
84	20.0
85	19.0
86	18.0
87	22.5
88	27.0
89	27.0
90	21.0
91	15.0
92	8.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	360703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.25627732511235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.03305276123999	13.9835266133079
2	10.331896256757682	4.185715117423476
3	4.652022172038596	2.8269795371815594
4	2.865941285157052	2.3221320587852055
5	1.9859029631150344	2.011350058081025
6	1.4849791281735443	1.804808942537212
7	1.083966331348799	1.536998583322013
8	0.8567713679600356	1.3883998746891488
9	0.724012865256963	1.3199224847034818
>10	5.968658044207213	24.42258589476661
>50	0.6473687812221994	8.9741421612795
>100	0.33258057893656334	11.872648688810463
>500	0.012317799219872715	1.8272650906701635
>1k	0.01368644357763635	5.68085100484332
>5k	0.0027372887155272703	3.7243937533095095
>10k+	0.0041059330732909054	12.118280136289412
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG	15797	4.379503358718946	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	14672	4.067612412427954	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	13242	3.671164365142513	TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	8193	2.2713977981885374	No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC	5241	1.4529959551209721	RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	3854	1.0684690728937658	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3500	0.9703273884608667	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3248	0.9004638164916843	No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG	1804	0.500134459652401	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1734	0.4807279118831837	No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA	1550	0.4297164148898124	No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT	1338	0.3709422987887542	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1305	0.3617934976975517	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1136	0.314940546654727	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1022	0.28333559743057307	No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	971	0.2691965411987147	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	883	0.2447997382888415	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	755	0.20931347951084409	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	735	0.203768751576782	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	727	0.20155086040315717	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	680	0.18852074975811126	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	661	0.18325325822075225	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	621	0.17216380235262807	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	558	0.15469790936033248	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	489	0.13556859798781823	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	487	0.13501412519441203	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	462	0.1280832152768344	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	459	0.12725150608672509	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	456	0.12641979689661578	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	452	0.12531085130980335	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	449	0.12447914211969405	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	431	0.11948888697903814	No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT	428	0.11865717778892883	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	425	0.11782546859881951	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	414	0.11477586823508538	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	410	0.11366692264827295	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	408	0.11311244985486675	No Hit
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT	397	0.11006284949113258	TruSeq Adapter, Index 11 (95% over 23bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	396	0.10978561309442948	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	375	0.10396364876366429	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	373	0.10340917597025807	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	369	0.10230023038344566	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	2.7723639670310476E-4	0.0
18	0.0	0.0	0.0	2.7723639670310476E-4	0.0
19	0.0	0.0	0.0	5.544727934062095E-4	0.0
20	0.0	0.0	0.0	5.544727934062095E-4	0.0
21	0.0	0.0	0.0	8.317091901093143E-4	0.0
22	0.0	0.0	0.0	0.001108945586812419	0.0
23	0.0	0.0	0.0	0.0016634183802186286	0.0
24	0.0	0.0	0.0	0.0038813095538434666	0.0
25	0.0	0.0	0.0	0.004435782347249676	0.0
26	0.0	0.0	0.0	0.004713018743952781	0.0
27	0.0	0.0	0.0	0.006099200727468305	0.0
28	0.0	0.0	0.0	0.015802474612076973	0.0
29	0.0	0.0	0.0	0.04019927752195019	0.0
30	0.0	0.0	0.0	0.07041804476258862	0.0
31	0.0	0.0	0.0	0.11782546859881952	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCTT	20	0.0018402624	37.0	29
GGTCGCT	20	0.0018402624	37.0	28
GCTCTAG	20	0.0018402624	37.0	1
TCTCGCG	20	0.0018402624	37.0	20
AGTTCCT	20	0.0018402624	37.0	5
CTCTCGC	20	0.0018402624	37.0	19
GATTACG	40	3.8207872E-8	37.0	1
CTTATAC	1720	0.0	34.52616	1
TTATACA	1760	0.0	33.741478	2
CGTCTTC	775	0.0	32.225807	37
TGATACA	35	2.3838002E-5	31.714285	2
AGACAGA	35	2.3838002E-5	31.714285	15
ACAGACA	35	2.3838002E-5	31.714285	17
CAGACAC	35	2.3838002E-5	31.714285	18
TATACAC	1915	0.0	31.203653	3
TCAGAGT	30	3.594627E-4	30.833332	3
AACTAGA	30	3.594627E-4	30.833332	27
TAACTAG	30	3.594627E-4	30.833332	26
TGATCAC	25	0.0054914053	29.6	26
ACTGCCA	25	0.0054914053	29.6	11
>>END_MODULE