FastQCFastQC Report
Fri 10 Feb 2017
ERR1630956.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630956.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730099
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT42900.5875915458040621No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG39030.5345850357280314No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT27110.3713195059848048No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26400.3615947974178844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24230.3318728008119447No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC23340.31968267317172055No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC13900.19038513954956793No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT13000.17805804418304913No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA12500.1712096578683165No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC12240.1676484969846555No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT11830.16203282020657472No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC10990.15052753119782386No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC10950.14997966029264526No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA10910.14943178938746662No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT10190.1395701130942516No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA9540.13066721088509914No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG9430.12916056589585798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8570.11738134143451778No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA8270.11327230964567819No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG7880.1079305683201867No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC7850.10751966514130275No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT7800.10683482650982949No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC7440.10190398836322197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG601.2696546E-930.8333341
TTACGGG604.318281E-827.7500023
GGTATCA3500.026.9571441
CTTATAC7000.026.6928581
GTATCAA11700.025.4572661
GCCGTCT4950.025.04040536
CCGTCTT4950.024.66666837
TATACAC8000.024.2812523
GCACCGC551.9014267E-523.54545410
TTAACGG551.9014267E-523.54545435
TTATACA8750.022.22
AACGGCC603.7238886E-521.58333437
CACCGCC603.7238886E-521.58333411
GTCGCCA2000.021.27500212
ACGGTAC806.9529597E-720.81253
TAATACG450.003824490420.5555574
ATTACGG909.466385E-820.5555572
AGGACCG450.003824490420.5555575
GGCGCAA450.003824490420.55555714
CGGTACT1001.2878445E-820.354
Produced by FastQC (version 0.11.5)