##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630956.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	730099
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.649281809727174	31.0	31.0	34.0	30.0	34.0
2	31.649243458763813	33.0	31.0	34.0	30.0	34.0
3	31.123250408506244	31.0	31.0	34.0	27.0	34.0
4	35.254440836105786	37.0	35.0	37.0	32.0	37.0
5	35.4512634587912	37.0	35.0	37.0	33.0	37.0
6	35.63680404986173	37.0	35.0	37.0	33.0	37.0
7	35.65390310081236	37.0	35.0	37.0	33.0	37.0
8	35.67501393646615	37.0	35.0	37.0	33.0	37.0
9	37.449259620955516	39.0	37.0	39.0	34.0	39.0
10	37.30411492140107	39.0	37.0	39.0	34.0	39.0
11	37.36181531545722	39.0	37.0	39.0	34.0	39.0
12	37.31201795920827	39.0	37.0	39.0	34.0	39.0
13	37.3628549004998	39.0	37.0	39.0	34.0	39.0
14	38.56650399466374	40.0	38.0	41.0	34.0	41.0
15	38.58271549474797	40.0	38.0	41.0	34.0	41.0
16	38.58514941124423	40.0	38.0	41.0	34.0	41.0
17	38.581945736126194	40.0	38.0	41.0	34.0	41.0
18	38.60678894232152	40.0	38.0	41.0	34.0	41.0
19	38.64812580211725	40.0	38.0	41.0	34.0	41.0
20	38.62561104726893	40.0	38.0	41.0	34.0	41.0
21	38.59096643057996	40.0	38.0	41.0	34.0	41.0
22	38.540636269875726	40.0	38.0	41.0	34.0	41.0
23	38.517551729286026	40.0	38.0	41.0	34.0	41.0
24	38.490721121382165	40.0	38.0	41.0	34.0	41.0
25	38.46630525449288	40.0	38.0	41.0	34.0	41.0
26	38.38103873584267	40.0	38.0	41.0	34.0	41.0
27	38.3177760824217	40.0	38.0	41.0	34.0	41.0
28	38.299553896115455	40.0	38.0	41.0	34.0	41.0
29	38.22502701688401	40.0	38.0	41.0	34.0	41.0
30	38.15695816594736	40.0	38.0	41.0	34.0	41.0
31	38.11375306636497	40.0	38.0	41.0	33.0	41.0
32	38.01514863052819	40.0	37.0	41.0	33.0	41.0
33	38.02242161679443	40.0	37.0	41.0	33.0	41.0
34	37.96759480563595	40.0	37.0	41.0	33.0	41.0
35	37.93003414605417	40.0	37.0	41.0	33.0	41.0
36	37.89749883235013	40.0	37.0	41.0	33.0	41.0
37	37.849360155266616	40.0	37.0	41.0	33.0	41.0
38	37.81257884204745	40.0	37.0	41.0	33.0	41.0
39	37.746286462520835	40.0	37.0	41.0	33.0	41.0
40	37.697561563568776	40.0	37.0	41.0	33.0	41.0
41	37.65203623070296	40.0	37.0	41.0	33.0	41.0
42	37.60861335243577	40.0	37.0	41.0	33.0	41.0
43	37.04353245244823	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	1.0
11	6.0
12	2.0
13	2.0
14	5.0
15	3.0
16	3.0
17	5.0
18	13.0
19	21.0
20	45.0
21	74.0
22	188.0
23	389.0
24	662.0
25	1192.0
26	1875.0
27	3095.0
28	4681.0
29	6867.0
30	9950.0
31	13309.0
32	17852.0
33	24010.0
34	32442.0
35	44639.0
36	62952.0
37	96196.0
38	172690.0
39	236926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.461770253075265	23.55927072903812	15.369011599796739	22.609947418089877
2	13.582678513461873	23.600771950105397	41.46533552299071	21.351214013442014
3	14.738960058841336	29.081124614607063	34.83418002216138	21.345735304390224
4	11.724711306274903	19.070290467457156	40.49409737583533	28.710900850432612
5	11.548570810259978	40.04977407173548	36.45012525698569	11.951529861018848
6	26.757056234839382	41.38260701630875	18.595834263572474	13.264502485279394
7	23.211783607428583	34.36150439871853	24.7662303331466	17.66048166070629
8	23.51735860479195	37.07935499158334	22.219041527244933	17.18424487637978
9	24.050436995530745	16.3177870398398	21.131106877286506	38.50066908734295
10	13.452833108934541	30.158238814188216	35.413142601208875	20.975785475668367
11	30.82280622216987	24.773763558092806	26.24616661576033	18.157263603976993
12	20.00536913487075	27.517227115774713	31.722547216199448	20.754856533155092
13	26.84663312783609	22.745956370300465	28.338485602637455	22.068924899225994
14	20.46599159839967	23.02358995149973	28.99141075388406	27.51900769621654
15	22.282320616793065	30.404095882887116	25.424634193445	21.88894930687482
16	22.191921917438595	29.889097232019218	25.491337476150495	22.427643374391693
17	20.636379449910216	30.17987971494277	27.23206030962924	21.951680525517773
18	21.271772732191113	28.392450886797544	28.68227459563703	21.653501785374313
19	22.833341779676456	28.218501874403334	29.236719951677788	19.711436394242423
20	22.544476844921032	27.218500504726073	28.98127514213826	21.25574750821464
21	21.977430458061168	28.3627288901916	27.87430197822487	21.78553867352236
22	20.08631706111089	30.50969799986029	28.16864562203208	21.235339316996736
23	21.19246841866651	28.170837105652797	28.430390946981166	22.206303528699532
24	22.30478332390539	28.91813302031642	28.238910065621237	20.53817359015695
25	21.552419603368858	28.05057944196609	28.62789840829805	21.769102546367
26	22.051256062533987	27.804585405540895	28.584342671336355	21.559815860588767
27	20.704589377604954	28.86183928480932	28.529692548544787	21.903878789040938
28	20.761157048564645	28.165495364327302	29.06140126202063	22.01194632508742
29	21.56241824738837	28.982644819401205	28.58269905862082	20.87223787458961
30	20.486810692796457	28.650908986315553	28.694738658729847	22.167541662158147
31	22.649120187810144	27.829376564000224	28.21137955263601	21.310123695553617
32	20.59720668018995	28.984699335295623	29.34752684225016	21.070567142264267
33	20.73718769646308	27.60433858969811	29.37519432296168	22.283279390877127
34	21.189181193235438	28.558044867887777	28.482301715246837	21.770472223629948
35	20.83101058897492	28.219186713034805	30.066881340749678	20.882921357240594
36	21.62021862788471	28.26753632041682	27.448195381722208	22.664049669976265
37	21.912644723523798	27.838279466209375	28.54968983658381	21.69938597368302
38	21.712808810859897	27.991820287385682	28.843759544938425	21.451611356815995
39	21.944832139203037	27.924432166048714	28.629678988739883	21.501056706008363
40	21.113848943773377	27.4612073157202	30.05989598670865	21.36504775379777
41	21.20246706268602	28.26246851454392	28.44449862278951	22.09056579998055
42	21.738010872498112	27.394504033014698	29.138514091924524	21.728971002562666
43	21.323957435909378	27.24931824314237	29.46641482867392	21.960309492274334
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	22.0
2	32.0
3	99.5
4	167.0
5	167.0
6	231.0
7	295.0
8	284.0
9	273.0
10	476.5
11	680.0
12	680.0
13	971.0
14	1262.0
15	3260.5
16	5259.0
17	5934.5
18	6610.0
19	6610.0
20	7191.0
21	7772.0
22	9997.0
23	12222.0
24	15040.5
25	17859.0
26	17859.0
27	21601.5
28	25344.0
29	31125.0
30	36906.0
31	38927.0
32	40948.0
33	40948.0
34	43878.5
35	46809.0
36	48180.5
37	49552.0
38	52822.5
39	56093.0
40	56093.0
41	58072.0
42	60051.0
43	55299.0
44	50547.0
45	50778.5
46	51010.0
47	51010.0
48	49697.5
49	48385.0
50	48154.0
51	47923.0
52	51057.0
53	54191.0
54	54191.0
55	43076.0
56	31961.0
57	30490.5
58	29020.0
59	23768.0
60	18516.0
61	18516.0
62	15129.0
63	11742.0
64	9669.0
65	7596.0
66	6096.5
67	4597.0
68	4597.0
69	3665.0
70	2733.0
71	2283.5
72	1834.0
73	1406.0
74	978.0
75	978.0
76	736.0
77	494.0
78	367.5
79	241.0
80	169.0
81	97.0
82	97.0
83	72.0
84	47.0
85	35.5
86	24.0
87	17.5
88	11.0
89	11.0
90	8.0
91	5.0
92	3.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	730099.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.84647465052615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.41342491457614	47.70038669306494
2	9.057277830064606	10.11634073478951
3	2.161983405051291	3.6221745427516536
4	0.9163501369821646	2.0469969878392247
5	0.46621090556094696	1.301811775960413
6	0.3109150239674449	1.0418104802679384
7	0.2179588069924604	0.8520561692694353
8	0.16287672250851265	0.7276872603785945
9	0.12500332484159324	0.628289551079777
>10	0.9352579297455859	10.439118160271692
>50	0.12543524096120043	4.886203497550357
>100	0.0977511363938669	10.43035492870795
>500	0.005879767602607215	2.2456013044452603
>1k	0.0036748547516295095	3.961167913623297
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	4290	0.5875915458040621	No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG	3903	0.5345850357280314	No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	2711	0.3713195059848048	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2640	0.3615947974178844	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2423	0.3318728008119447	No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC	2334	0.31968267317172055	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1390	0.19038513954956793	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1300	0.17805804418304913	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1250	0.1712096578683165	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1224	0.1676484969846555	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1183	0.16203282020657472	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1099	0.15052753119782386	No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	1095	0.14997966029264526	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1091	0.14943178938746662	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1019	0.1395701130942516	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	954	0.13066721088509914	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	943	0.12916056589585798	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	857	0.11738134143451778	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	827	0.11327230964567819	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	788	0.1079305683201867	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	785	0.10751966514130275	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	780	0.10683482650982949	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	744	0.10190398836322197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	1.3696772629465318E-4	0.0
16	0.0	0.0	0.0	1.3696772629465318E-4	0.0
17	0.0	0.0	0.0	1.3696772629465318E-4	0.0
18	0.0	0.0	0.0	2.7393545258930636E-4	0.0
19	0.0	0.0	0.0	5.478709051786127E-4	0.0
20	0.0	0.0	0.0	5.478709051786127E-4	0.0
21	0.0	0.0	0.0	8.218063577679191E-4	0.0
22	0.0	0.0	0.0	0.0015066449892411852	0.0
23	0.0	0.0	0.0	0.0017805804418304914	0.0
24	0.0	0.0	0.0	0.002054515894419798	0.0
25	0.0	0.0	0.0	0.002054515894419798	0.0
26	0.0	0.0	0.0	0.002054515894419798	0.0
27	0.0	0.0	0.0	0.0030132899784823704	0.0
28	0.0	0.0	0.0	0.0064374831358487	0.0
29	0.0	0.0	0.0	0.013833740355759972	0.0
30	0.0	0.0	0.0	0.025475997090805492	0.0
31	0.0	0.0	0.0	0.05958096093817414	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	60	1.2696546E-9	30.833334	1
TTACGGG	60	4.318281E-8	27.750002	3
GGTATCA	350	0.0	26.957144	1
CTTATAC	700	0.0	26.692858	1
GTATCAA	1170	0.0	25.457266	1
GCCGTCT	495	0.0	25.040405	36
CCGTCTT	495	0.0	24.666668	37
TATACAC	800	0.0	24.281252	3
GCACCGC	55	1.9014267E-5	23.545454	10
TTAACGG	55	1.9014267E-5	23.545454	35
TTATACA	875	0.0	22.2	2
AACGGCC	60	3.7238886E-5	21.583334	37
CACCGCC	60	3.7238886E-5	21.583334	11
GTCGCCA	200	0.0	21.275002	12
ACGGTAC	80	6.9529597E-7	20.8125	3
TAATACG	45	0.0038244904	20.555557	4
ATTACGG	90	9.466385E-8	20.555557	2
AGGACCG	45	0.0038244904	20.555557	5
GGCGCAA	45	0.0038244904	20.555557	14
CGGTACT	100	1.2878445E-8	20.35	4
>>END_MODULE