FastQCFastQC Report
Fri 10 Feb 2017
ERR1630955.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630955.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891826
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT249442.796958151029461No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG197602.2156788431824146No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT170301.9095653187953705TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC105331.1810599825526504No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC61500.6895964010916928RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC51220.5743272790880732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33250.37283057457396396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31410.3521987472892695No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG24400.27359597051442774No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA20700.23210805695281367No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA20040.22470751020939062No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16660.18680774052337565No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT15560.1744734959510039No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCT13500.151374819751835No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT13010.14588047444232397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11890.13332197087772726No Hit
TCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGT10240.11482060401916967No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9550.1070836687874092No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9250.10371978390403509No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAAC9010.10102867599733581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG900.034.9444471
CTTATAC28350.034.128751
CGTCTTC6450.032.69767437
TTATACA30200.031.9155642
TACGAAC352.3877132E-531.71428528
CCGCCAA303.598837E-430.83333431
TATACAC31850.030.7268473
TTACGGG1050.029.9523833
ACGAACG405.93866E-527.7529
ATTACGG1150.027.3478242
CCGTCTT24250.026.92989537
GCCGTCT24950.026.100236
GGTATCA6250.026.0480021
CGAACGC451.3228871E-424.66666830
GTATCAA17100.023.3684221
AGGACCG603.7250804E-521.5833345
TGCCGTC30450.021.44663435
GCTTTAT5150.020.8349511
ATGCCGT31950.020.49765234
CTGCGAC555.141852E-420.18181818
Produced by FastQC (version 0.11.5)