##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630955.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	891826
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.483900446948173	31.0	31.0	34.0	28.0	34.0
2	31.491601500741176	31.0	31.0	34.0	28.0	34.0
3	30.99054748347772	31.0	31.0	34.0	27.0	34.0
4	35.11989222112834	37.0	35.0	37.0	32.0	37.0
5	35.31489438522761	37.0	35.0	37.0	33.0	37.0
6	35.496754972382504	37.0	35.0	37.0	33.0	37.0
7	35.49685140374916	37.0	35.0	37.0	33.0	37.0
8	35.52669915431934	37.0	35.0	37.0	33.0	37.0
9	37.29500373391222	39.0	37.0	39.0	34.0	39.0
10	37.13478862468688	39.0	37.0	39.0	33.0	39.0
11	37.188454922821265	39.0	37.0	39.0	33.0	39.0
12	37.118175518542856	39.0	37.0	39.0	33.0	39.0
13	37.212725352254814	39.0	37.0	39.0	34.0	39.0
14	38.30151621504643	40.0	38.0	41.0	34.0	41.0
15	38.319354896582965	40.0	38.0	41.0	34.0	41.0
16	38.30116300713368	40.0	38.0	41.0	33.0	41.0
17	38.272336756273084	40.0	38.0	41.0	33.0	41.0
18	38.33292817208738	40.0	38.0	41.0	34.0	41.0
19	38.3227456925454	40.0	38.0	41.0	34.0	41.0
20	38.355487505410245	40.0	38.0	41.0	34.0	41.0
21	38.30512005705149	40.0	38.0	41.0	34.0	41.0
22	38.234659002989375	40.0	38.0	41.0	34.0	41.0
23	38.26036693256308	40.0	38.0	41.0	34.0	41.0
24	38.1838217320419	40.0	38.0	41.0	33.0	41.0
25	38.1742514795487	40.0	38.0	41.0	33.0	41.0
26	38.05804831884247	40.0	37.0	41.0	33.0	41.0
27	37.96836042008194	40.0	37.0	41.0	33.0	41.0
28	37.99095787743349	40.0	37.0	41.0	33.0	41.0
29	37.90047273795561	40.0	37.0	41.0	33.0	41.0
30	37.860600610432975	40.0	37.0	41.0	33.0	41.0
31	37.81826499788075	40.0	37.0	41.0	33.0	41.0
32	37.71659494116565	40.0	37.0	41.0	33.0	41.0
33	37.6674878283432	40.0	37.0	41.0	33.0	41.0
34	37.56429841695577	40.0	37.0	41.0	32.0	41.0
35	37.55902945193345	40.0	37.0	41.0	32.0	41.0
36	37.53301092365551	40.0	37.0	41.0	32.0	41.0
37	37.44657253769233	39.0	37.0	41.0	32.0	41.0
38	37.4202826560338	39.0	37.0	41.0	32.0	41.0
39	37.32944206605325	39.0	36.0	41.0	31.0	41.0
40	37.199797942648004	39.0	36.0	41.0	31.0	41.0
41	37.18273071204472	39.0	36.0	41.0	31.0	41.0
42	37.11968926674037	39.0	36.0	41.0	31.0	41.0
43	36.42362187242803	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	15.0
10	22.0
11	28.0
12	39.0
13	16.0
14	15.0
15	18.0
16	16.0
17	17.0
18	28.0
19	40.0
20	83.0
21	161.0
22	329.0
23	604.0
24	1100.0
25	1895.0
26	3105.0
27	4980.0
28	7349.0
29	10701.0
30	14604.0
31	19689.0
32	25412.0
33	33372.0
34	44473.0
35	59528.0
36	82392.0
37	122112.0
38	213101.0
39	246575.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.59828262463754	22.71440841599146	15.758230865662137	24.929078093708863
2	12.993117491528617	21.702888231560866	41.36221639647196	23.94177788043856
3	13.733172165870922	28.3902913797086	36.091905820193624	21.784630634226858
4	10.781587439702363	19.675362682855177	37.61204539899038	31.931004478452074
5	12.331329205472818	37.812308679047256	35.690033706126535	14.166328409353394
6	26.314101629690096	40.956756138529265	17.386239019719092	15.342903212061545
7	23.459845306147166	34.21295185383696	21.86345766999392	20.463745170021955
8	25.149748942058203	34.04161798377711	23.065037350335153	17.743595723829536
9	23.17369083206814	14.413686077777504	19.249943374604463	43.16267971554989
10	14.270384581745766	28.787454054938966	33.05992424531243	23.882237118002838
11	30.315554827959712	22.458528905862803	25.976255457903225	21.24966080827426
12	19.845687387450017	26.833933973667506	27.831774359572382	25.48860427931009
13	29.281496614810514	19.721223646765175	27.166510059137096	23.830769679287215
14	21.362350951867292	23.135118285405447	26.327220780735257	29.175309981992005
15	24.22905364947871	28.58382688999872	22.084577036327715	25.10254242419485
16	25.184845474341405	27.18792679289458	23.091948429402148	24.535279303361868
17	20.45163518444181	31.839058291639848	24.228717260990372	23.48058926292797
18	24.328288253538247	25.20166489875828	25.41056215001581	25.05948469768767
19	23.384942802744032	28.897565220121415	25.78597170300036	21.931520274134193
20	22.22059011511214	25.08998392063026	26.51066463637526	26.178761327882345
21	24.200684886962254	25.3052725531662	25.629999573907913	24.86404298596363
22	19.884035675120483	31.26820702693126	28.130263078223777	20.717494219724475
23	22.775406861876643	25.104560755124876	25.62293541565283	26.497096967345644
24	23.457154198240467	29.896078383002962	26.02693799014606	20.619829428610515
25	23.606622816558385	27.55100210130676	26.299188406707135	22.54318667542772
26	25.163765129072264	28.524510386555225	24.989067374129036	21.32265711024348
27	20.731061888754084	29.825997447932668	29.09491313327936	20.348027530033885
28	20.504336047614668	25.177781316086325	27.35331779966047	26.964564836638537
29	20.62195988903665	29.45798844169154	28.22243352402823	21.69761814524358
30	21.96953217331632	24.717713993536854	30.40963147519808	22.90312235794875
31	22.54228963945882	26.659124089228172	25.16152253915001	25.637063732163
32	23.829984772814427	25.82286230722136	29.84494733277568	20.50220558718853
33	22.0475743026106	25.558573084884273	28.92100028480892	23.47285232769621
34	21.434562347363723	31.03621109947456	25.71387243700004	21.81535411616167
35	23.903317463271982	24.985030712268987	31.057403574239817	20.054248250219214
36	25.61082543007268	25.301572279794488	25.050962855983116	24.036639434149713
37	21.973008187695804	26.470522276766996	28.664784386191926	22.891685149345275
38	21.919858806538496	23.785245103865552	29.407530168440932	24.887365921155023
39	24.72746925969864	24.91528616568703	26.781233110494647	23.576011464119684
40	20.482470795872736	23.901074873349735	33.8141072361649	21.802347094612625
41	22.028960806255927	25.43590341613723	26.255457903223274	26.279677874383566
42	22.664510790221414	24.29431301621617	30.98025848091444	22.06091771264798
43	22.7561205885453	23.91587596683658	30.671453848620693	22.656549595997426
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	17.5
2	26.0
3	131.5
4	237.0
5	237.0
6	282.0
7	327.0
8	333.5
9	340.0
10	518.0
11	696.0
12	696.0
13	1035.0
14	1374.0
15	3288.5
16	5203.0
17	6007.0
18	6811.0
19	6811.0
20	7485.5
21	8160.0
22	10048.0
23	11936.0
24	15341.0
25	18746.0
26	18746.0
27	21120.5
28	23495.0
29	26478.0
30	29461.0
31	33576.0
32	37691.0
33	37691.0
34	40884.0
35	44077.0
36	47394.5
37	50712.0
38	51235.5
39	51759.0
40	51759.0
41	52681.0
42	53603.0
43	54991.0
44	56379.0
45	56957.0
46	57535.0
47	57535.0
48	59998.5
49	62462.0
50	81675.5
51	100889.0
52	77509.0
53	54129.0
54	54129.0
55	65408.0
56	76687.0
57	63603.0
58	50519.0
59	39485.5
60	28452.0
61	28452.0
62	25014.0
63	21576.0
64	18375.0
65	15174.0
66	12578.5
67	9983.0
68	9983.0
69	8113.5
70	6244.0
71	5221.0
72	4198.0
73	2861.0
74	1524.0
75	1524.0
76	1101.0
77	678.0
78	507.5
79	337.0
80	265.0
81	193.0
82	193.0
83	147.0
84	101.0
85	84.5
86	68.0
87	43.0
88	18.0
89	18.0
90	17.5
91	17.0
92	8.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	891826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.39044745210671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82258075745098	34.20147048395614
2	12.315463453460579	10.68746939651444
3	3.5055255701800707	4.563189691347444
4	1.4917737398585869	2.5891492027906686
5	0.7870951990811268	1.7076206437767547
6	0.4901640286663878	1.2761061917257097
7	0.34888180369515875	1.0596696299161696
8	0.24593290216577873	0.8536910934534658
9	0.20151299368772185	0.7869365067171427
>10	1.4718571543860182	12.934797707004977
>50	0.18873158435766096	5.720268759090544
>100	0.1183129594159456	9.59745836208685
>500	0.00776671506002041	2.2715007027196408
>1k	0.002847795522007484	2.3664227191282308
>5k	5.177810040013607E-4	1.2662259987664206
>10k+	0.0010355620080027214	8.118022911005404
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	24944	2.796958151029461	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	19760	2.2156788431824146	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	17030	1.9095653187953705	TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	10533	1.1810599825526504	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	6150	0.6895964010916928	RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	5122	0.5743272790880732	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3325	0.37283057457396396	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3141	0.3521987472892695	No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG	2440	0.27359597051442774	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2070	0.23210805695281367	No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA	2004	0.22470751020939062	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1666	0.18680774052337565	No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT	1556	0.1744734959510039	No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCT	1350	0.151374819751835	No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT	1301	0.14588047444232397	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1189	0.13332197087772726	No Hit
TCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGT	1024	0.11482060401916967	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	955	0.1070836687874092	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	925	0.10371978390403509	No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAAC	901	0.10102867599733581	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.363884883374111E-4	0.0
11	0.0	0.0	0.0	4.485179844498815E-4	0.0
12	0.0	0.0	0.0	5.606474805623519E-4	0.0
13	0.0	0.0	0.0	6.727769766748222E-4	0.0
14	0.0	0.0	0.0	6.727769766748222E-4	0.0
15	0.0	0.0	0.0	8.97035968899763E-4	0.0
16	0.0	0.0	0.0	8.97035968899763E-4	0.0
17	0.0	0.0	0.0	8.97035968899763E-4	0.0
18	0.0	0.0	0.0	0.0010091654650122334	0.0
19	0.0	0.0	0.0	0.0011212949611247038	0.0
20	0.0	0.0	0.0	0.0011212949611247038	0.0
21	0.0	0.0	0.0	0.0013455539533496444	0.0
22	0.0	0.0	0.0	0.0020183309300244668	0.0
23	0.0	0.0	0.0	0.002130460426136937	0.0
24	0.0	0.0	0.0	0.0028032374028117593	0.0
25	0.0	0.0	0.0	0.0029153668989242297	0.0
26	0.0	0.0	0.0	0.0031396258911491706	0.0
27	0.0	0.0	0.0	0.004485179844498815	0.0
28	0.0	0.0	0.0	0.011549338099584448	0.0
29	0.0	0.0	0.0	0.026350431586430537	0.0
30	0.0	0.0	0.0	0.04630948189445026	0.0
31	0.0	0.0	0.0	0.09261896378890053	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	90	0.0	34.944447	1
CTTATAC	2835	0.0	34.12875	1
CGTCTTC	645	0.0	32.697674	37
TTATACA	3020	0.0	31.915564	2
TACGAAC	35	2.3877132E-5	31.714285	28
CCGCCAA	30	3.598837E-4	30.833334	31
TATACAC	3185	0.0	30.726847	3
TTACGGG	105	0.0	29.952383	3
ACGAACG	40	5.93866E-5	27.75	29
ATTACGG	115	0.0	27.347824	2
CCGTCTT	2425	0.0	26.929895	37
GCCGTCT	2495	0.0	26.1002	36
GGTATCA	625	0.0	26.048002	1
CGAACGC	45	1.3228871E-4	24.666668	30
GTATCAA	1710	0.0	23.368422	1
AGGACCG	60	3.7250804E-5	21.583334	5
TGCCGTC	3045	0.0	21.446634	35
GCTTTAT	515	0.0	20.834951	1
ATGCCGT	3195	0.0	20.497652	34
CTGCGAC	55	5.141852E-4	20.181818	18
>>END_MODULE