FastQCFastQC Report
Fri 10 Feb 2017
ERR1630954.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630954.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549462
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT167463.047708485755157No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG138472.5201014810851343No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT105341.917148046634708RNA PCR Primer, Index 29 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT99551.811772242666463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT98611.794664599189753No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC76361.3897230381718846No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA64211.1685976464250485No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA39710.7227069387873957No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC39060.7108771853194579RNA PCR Primer, Index 29 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34530.628432903458292No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC34320.624610983107112RNA PCR Primer, Index 29 (95% over 24bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT33800.6151471803327618No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA27510.5006715660045645No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT27090.49302772530220473No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT26770.48720384667183536No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC26140.4757380856182957No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG25880.4710061842311207No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC23580.42914705657534097No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG23520.4280550793321467No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG18430.33541900986783435No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG18410.33505501745343624No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG17870.32522722226468803No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT17280.3144894460399445No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG15730.286280033924093No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG15610.2840960794377045No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG15340.2791821818433304No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG15240.27736221977133996No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG15190.27645223873534475No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG14740.2682624094113879No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG13870.2524287393850712No Hit
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTA13730.24988079248428463No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA13650.2484248228266923No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC13440.2446029024755124No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG13400.24387491764671626No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG13190.24005299729553636No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA13060.23768704660194884No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12010.2185774448460494No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA11790.21457352828767048No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT11760.21402753966607335No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTAT11720.2132995548372772No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC11450.20838565724290306No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT11280.20529172172051935No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10710.19491793791017395No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG10390.1890940592798046No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10120.18418016168543047No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10040.18272419202783813No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA9420.17144042718149755No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9180.16707251820872052No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8910.1621586206143464No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG8890.1617946281999483No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC8880.16161263199274928No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA8800.16015666233515694No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.15779071164156938No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA8600.1565167381911761No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG8270.15051086335360772No Hit
ACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT8230.14978287852481154No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG8230.14978287852481154No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT8190.14905489369601538No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC8170.14869090128161727No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG7890.1435950074800441No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT7830.14250303023684985No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC7700.14013707954326232No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG7650.1392270985072671No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG7580.13795312505687382No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC7550.1374071364352767No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7500.13649715539928148No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA7440.1354051781560872No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA7430.1352231819488882No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC7220.1314012615977083No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7220.1314012615977083No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG7180.13067327676891213No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA7150.130127288147315No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7140.12994529194011595No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC7100.1292173071113198No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG7100.1292173071113198No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA7020.12776133745372745No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6860.12484939813854279No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG6780.12339342848095046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC6630.12066348537296483No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG6590.11993550054416867No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA6360.1157495877785907No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG6250.11374762949940125No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6240.11356563329220218No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTG6130.11156367501301272No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6000.10919772431942519No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA5980.1088337319050271No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA5960.108469739490629No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5770.1050118115538472No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT5720.10410183051785202No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA5680.10337384568905583No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT5630.10246386465306062No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5630.10246386465306062No Hit
GTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT5550.1010078949954683No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5530.10064390258107021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAAGT308.304087E-637.032
AAGTTTG200.001841100237.035
GATTACG1100.037.01
ATTACGG1150.035.3913042
TTACGGG1150.035.3913043
ACTCAGA950.035.052631
CTTATAC19100.034.6753961
TCAGAGT950.033.1052633
GTTTGTG451.07822416E-732.888891
AGCAACG401.598055E-632.3752
TTATACA20650.031.983052
TGTATCC352.3860553E-531.7142873
GATTAGG352.3860553E-531.7142871
CAGACAC352.3860553E-531.71428718
CGTCTTC5250.031.36190637
TTATAGT303.597055E-430.83333212
TATACAC21950.030.4259663
CTCAGAG1100.030.2727282
TCTGAGG250.00549387829.628
AGTTTGC250.00549387829.636
Produced by FastQC (version 0.11.5)