FastQCFastQC Report
Fri 10 Feb 2017
ERR1630953.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630953.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936855
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT216092.306546904270138No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT170491.8198120306771057TruSeq Adapter, Index 8 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG169611.810418901537591No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC87450.9334422082392687No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC68410.7302090504934061RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC47070.5024256688601758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30590.3265179777019923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30160.3219281532360931No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG20660.2205250545708781No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA17970.19181196663304353No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17030.18177839687038017No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT13830.1476215636357814No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT12020.1283016048374615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10850.11581301268606134No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC9930.10599292313111422No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9590.1023637595999381No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9530.10172331897678936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC24700.033.854251
CGTCTTC9700.031.2783537
TTATACA27300.031.0366332
TATACAC28550.029.612963
GGTATCA6150.028.2764231
GATTACG601.3369645E-624.6666681
CCGTCTT27800.024.2230237
GATACAC1150.024.1304363
GCCGTCT28850.023.34142136
GTATCAA14900.021.6040271
TTACGGG705.1002626E-621.1428573
TTGCTCG450.003825287620.55555520
TGCCGTC33800.019.9778135
GCTCTAG851.2448054E-619.5882341
ATGCCGT35100.019.23789234
TGGGACG1355.638867E-1119.18518611
TTTGACG609.235075E-418.531
TATGCCG37650.018.08233833
GGGACGA1653.6379788E-1217.93939412
ATACACA48900.017.9325164
Produced by FastQC (version 0.11.5)