FastQCFastQC Report
Fri 10 Feb 2017
ERR1630952.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630952.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675096
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT195122.8902556080912936No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG157802.3374453411070424No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT122881.8201855736073091TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC87441.2952231978859303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77041.14117103345302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT76401.1316909002571487No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA48160.713380022989323No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC43580.6455378198063683RNA PCR Primer, Index 6 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC42650.6317620012561176No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA27770.4113489044521076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27180.4026094066621636No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT25160.37268773626269447No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA22600.33476720347920885No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC22200.32884212023178927No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG20360.301586737293659No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC20120.29803168734520724No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT20110.29788356026402174No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT19290.28573713960681146No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG19190.28425586879495657No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC17700.26218493369831847No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG16140.23907710903338192No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA15560.23048573832462346No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG14100.2088591844715418No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG13150.19478711175892022No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT12980.19226895137876687No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT12570.18619574105016176No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG11970.17730811617903233No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG11720.17360493914939504No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG11570.1713830329316127No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG10940.16205102681692676No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10920.1617547726545558No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG10900.16145851849218482No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC10810.1601253747615154No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG10680.15819972270610402No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG10630.15745908730017658No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG10600.1570147060566201No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG10030.14857146242904712No Hit
ACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT10000.14812708118549064No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA9610.14235012501925653No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA9550.1414613625321436No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9530.14116510836977259No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT9470.14027634588265966No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9440.13983196463910316No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA9390.13909132923317571No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC9380.13894320215199024No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG9160.13568440636590942No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG9100.1347956438787965No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG8930.13227748349864316No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT8730.12931494187493334No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG8230.12190858781565882No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG8190.12131607949091684No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA8100.11998293576024742No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7890.11687226705535211No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT7830.11598350456823918No Hit
TATCAACGCAGAGTACGGGAGCCCTCCAGGACAGGCTGCATCA7580.11228032753860193No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT7430.11005842132081955No Hit
GTATCAACGCAGAGTACGGGAGCCCTCCAGGACAGGCTGCATC7380.10931778591489211No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT7320.10842902342777916No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT7320.10842902342777916No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7300.10813276926540819No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC7220.10694775261592426No Hit
GTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGTTC7200.10665149845355328No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7180.10635524429118229No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG7040.10428146515458542No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA6860.10161517769324659No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG6760.10013390688139168No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAGC200.001841398337.03
TAGTTCC651.8189894E-1234.1538474
CTTATAC24400.033.2848361
ATTACGG900.032.888892
TTATACA25300.032.1007922
TATACAC26550.030.6591323
CGTCTTC6550.030.22137537
TCGCCGA250.00549475629.61
CCAAGCT250.00549475629.624
CTATTAC250.00549475629.61
GGTTGCC250.00549475629.637
GATTACG1000.029.61
GGTATCA10650.028.835681
AGATTAC405.936555E-527.755
GCCTAGT803.274181E-1127.751
CCTAGTT905.456968E-1226.7222232
TATAGTG358.865588E-426.428573
CTAGTTC856.91216E-1126.1176473
AGTTCCT856.91216E-1126.1176475
GTTTGTG509.083831E-625.9000021
Produced by FastQC (version 0.11.5)