FastQCFastQC Report
Fri 10 Feb 2017
ERR1630951.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630951.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904729
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT218682.4170773789720457No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG166601.841435391150278No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT145831.6118638841023114Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC86440.9554242209545621No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC57430.6347757173695107Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC41790.4619062724860151No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27280.3015267555256878No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26720.29533705673190536No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG19890.21984483751488013No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17540.19387020864811452No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA17320.19143854126484283No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT13920.15385822715973513No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT10530.11638844339023066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10230.11307253332213292No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9940.10986715358963844No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9520.1052248794943016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC24400.034.0430341
TTATACA26900.030.6728632
CGTCTTC7050.030.43971637
TATACAC28700.029.0714283
TTACGGG754.620233E-1027.1333333
TATAGCG358.867931E-426.428575
GGTATCA5000.025.91
GATTACG809.786163E-1025.4375021
CCGTCTT21150.025.3664337
GCCGTCT21900.024.49771736
CGTCAAT551.902078E-523.54545430
GTATCAA13500.023.4333321
CCGTAGA400.001930870723.1250022
TGCGGGT603.725157E-521.58333421
GCGGGTA603.725157E-521.58333422
TGCCGTC26250.020.29714435
CTCTATA1101.7535058E-920.1818182
ATGCCGT27300.019.58424834
CGGTCCG609.234743E-418.521
CTGTGCG500.00703375318.59
Produced by FastQC (version 0.11.5)