##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630951.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	904729
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.497967899779933	31.0	31.0	34.0	28.0	34.0
2	31.505301587547212	31.0	31.0	34.0	30.0	34.0
3	30.99905496563059	31.0	31.0	34.0	27.0	34.0
4	35.12579678555678	37.0	35.0	37.0	32.0	37.0
5	35.32098893701871	37.0	35.0	37.0	33.0	37.0
6	35.49800548009404	37.0	35.0	37.0	33.0	37.0
7	35.49791152930877	37.0	35.0	37.0	33.0	37.0
8	35.5291363491167	37.0	35.0	37.0	33.0	37.0
9	37.309174349446074	39.0	37.0	39.0	34.0	39.0
10	37.144858847234914	39.0	37.0	39.0	33.0	39.0
11	37.20779039911399	39.0	37.0	39.0	33.0	39.0
12	37.1315355205813	39.0	37.0	39.0	33.0	39.0
13	37.21949556165438	39.0	37.0	39.0	34.0	39.0
14	38.337326425924225	40.0	38.0	41.0	34.0	41.0
15	38.35784969863904	40.0	38.0	41.0	34.0	41.0
16	38.35581262455387	40.0	38.0	41.0	34.0	41.0
17	38.31369282956554	40.0	38.0	41.0	33.0	41.0
18	38.3555152979511	40.0	38.0	41.0	34.0	41.0
19	38.37264639466625	40.0	38.0	41.0	34.0	41.0
20	38.39422744269278	40.0	38.0	41.0	34.0	41.0
21	38.343267431462905	40.0	38.0	41.0	34.0	41.0
22	38.2754018054025	40.0	38.0	41.0	34.0	41.0
23	38.27512879547356	40.0	38.0	41.0	34.0	41.0
24	38.22965440479967	40.0	38.0	41.0	34.0	41.0
25	38.204895609624536	40.0	38.0	41.0	33.0	41.0
26	38.06431539168082	40.0	37.0	41.0	33.0	41.0
27	38.01346812139326	40.0	37.0	41.0	33.0	41.0
28	38.0101588431453	40.0	37.0	41.0	33.0	41.0
29	37.92988066039665	40.0	37.0	41.0	33.0	41.0
30	37.88103177857679	40.0	37.0	41.0	33.0	41.0
31	37.84552169765753	40.0	37.0	41.0	33.0	41.0
32	37.749809058845244	40.0	37.0	41.0	33.0	41.0
33	37.684971963980374	40.0	37.0	41.0	33.0	41.0
34	37.6182193783995	40.0	37.0	41.0	32.0	41.0
35	37.63580475479398	40.0	37.0	41.0	33.0	41.0
36	37.552926898551945	40.0	37.0	41.0	32.0	41.0
37	37.49913620542726	40.0	37.0	41.0	32.0	41.0
38	37.48082464472787	40.0	37.0	41.0	32.0	41.0
39	37.373582586608805	39.0	36.0	41.0	32.0	41.0
40	37.261374400511095	39.0	36.0	41.0	31.0	41.0
41	37.228301513491886	39.0	36.0	41.0	31.0	41.0
42	37.16714950001602	39.0	36.0	41.0	31.0	41.0
43	36.48202500417252	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	11.0
10	20.0
11	21.0
12	17.0
13	16.0
14	16.0
15	12.0
16	12.0
17	15.0
18	17.0
19	41.0
20	77.0
21	167.0
22	325.0
23	620.0
24	1126.0
25	1900.0
26	3039.0
27	5101.0
28	7351.0
29	10760.0
30	14572.0
31	19591.0
32	25735.0
33	33471.0
34	44408.0
35	59249.0
36	82609.0
37	122846.0
38	214065.0
39	257509.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.235569988361156	22.266004516269515	15.20753728464546	25.29088821072387
2	13.287625355216868	21.480354890801557	41.731501919359275	23.5005178346223
3	14.327494752572317	28.450066262936197	34.58715261697149	22.635286367519996
4	11.36616600108983	19.64743033549273	36.80560698286448	32.18079668055296
5	12.708335866320192	37.687639060978476	35.47725340958453	14.126771663116799
6	27.679669823781488	39.781415208311	17.576755028301292	14.962159939606224
7	24.171989623412095	33.31461686317118	22.188743811682833	20.32464970173389
8	24.721878043038302	35.22999704884004	22.39576713026774	17.652357777853922
9	23.309963536042286	14.821675883054484	19.393210563605233	42.475150017298
10	14.376901812586972	28.7250657379171	33.074213383234095	23.82381906626183
11	31.21619844174333	22.541777703599642	25.42031923371529	20.82170462094174
12	20.27336362601398	26.239017429528623	28.728934299663212	24.758684644794187
13	29.26180104760652	20.289611585347657	26.741267274509827	23.707320092535998
14	21.608238489094525	22.45423767780186	26.089801476464224	29.847722356639387
15	24.18105311093156	28.385848137950703	22.345475827568258	25.087622923549485
16	25.353116789668505	26.946522107725073	23.136651969816377	24.56370913279004
17	20.77848725972087	31.078809234588476	24.365749301724605	23.77695420396605
18	24.19055871979344	25.57848814396355	25.329242237178207	24.901710899064803
19	23.120625071153903	28.78442052813605	24.99190365291706	23.103050747792985
20	21.96503041242184	25.29895692522291	25.768600321201156	26.96741234115409
21	24.230349640610612	25.375443917460366	26.72479825450494	23.66940818742408
22	21.549989002231605	30.295591276503792	25.088838757241117	23.06558096402348
23	24.64572264180766	24.730941530557768	25.915163546211073	24.708172281423497
24	23.332401194169748	27.80324273898593	25.574840642888645	23.28951542395568
25	21.619070461983643	28.033477428047515	27.00444000358118	23.343012106387658
26	23.53036102523518	29.306786894197046	25.87913065680441	21.283721423763357
27	21.164127600640633	29.087273647688978	25.96224946917806	23.786349282492324
28	21.352139701501777	25.554834652144454	28.931425874488383	24.16159977186539
29	21.09791992961428	27.764557121524785	25.64016407123017	25.49735887763076
30	22.670213953570627	24.992124713588268	28.55075939867076	23.78690193417034
31	22.36935038005856	25.279614116492343	27.42732906759925	24.92370643584985
32	22.14607910213998	25.463536594936166	28.629567527955885	23.760816774967974
33	23.261440718712453	27.063684263464527	28.208557479643076	21.46631753817994
34	20.925713666744407	27.92250497110184	26.451788325564895	24.699993036588857
35	21.187228440781713	25.400865894649115	32.711342291448595	20.70056337312057
36	22.870826512690538	27.948148008961798	25.210532656740302	23.970492821607355
37	21.954640560875134	26.968517644510126	27.706197104326268	23.370644690288472
38	21.983378448131983	24.643843626102402	28.395685337819394	24.977092587946224
39	24.483464109142076	25.06175882501832	26.527943726795538	23.92683333904407
40	21.069182042357433	24.613779374818314	32.203013278009216	22.114025304815033
41	22.603011509523846	25.71068242534505	25.91394771251944	25.77235835261167
42	23.164616144724	24.662965374161764	29.949189204723183	22.223229276391052
43	23.181306225400093	24.19486940288197	29.499551799489126	23.12427257222881
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	12.5
2	18.0
3	79.0
4	140.0
5	140.0
6	196.5
7	253.0
8	255.0
9	257.0
10	399.5
11	542.0
12	542.0
13	801.0
14	1060.0
15	2755.0
16	4450.0
17	4950.0
18	5450.0
19	5450.0
20	5565.0
21	5680.0
22	6842.5
23	8005.0
24	10643.5
25	13282.0
26	13282.0
27	16615.5
28	19949.0
29	23459.5
30	26970.0
31	31437.5
32	35905.0
33	35905.0
34	40529.5
35	45154.0
36	48868.5
37	52583.0
38	55347.5
39	58112.0
40	58112.0
41	59320.0
42	60528.0
43	61903.0
44	63278.0
45	64374.0
46	65470.0
47	65470.0
48	66894.0
49	68318.0
50	83744.5
51	99171.0
52	78267.0
53	57363.0
54	57363.0
55	65945.5
56	74528.0
57	62882.5
58	51237.0
59	39693.0
60	28149.0
61	28149.0
62	24578.5
63	21008.0
64	17567.0
65	14126.0
66	11916.5
67	9707.0
68	9707.0
69	7911.5
70	6116.0
71	4870.5
72	3625.0
73	2790.0
74	1955.0
75	1955.0
76	1528.5
77	1102.0
78	850.5
79	599.0
80	465.0
81	331.0
82	331.0
83	237.5
84	144.0
85	117.0
86	90.0
87	64.0
88	38.0
89	38.0
90	30.5
91	23.0
92	14.0
93	5.0
94	3.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	904729.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.080740374649594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.49400547984531	44.580826826561776
2	12.653077192604497	14.191878739577056
3	3.569236190014503	6.004962243240204
4	1.5028030075392615	3.3711322120020757
5	0.7336410047681736	2.0571565358300505
6	0.44293773548974136	1.4904165687681241
7	0.29807094159677117	1.1701227362241107
8	0.20762032327896646	0.9314801157046828
9	0.15173982153610144	0.7658713382455592
>10	0.8321573486616277	8.611651979481312
>50	0.0738566112514916	2.8368596200444243
>100	0.03594396562759547	3.6261725371873514
>500	0.0021605662258011808	0.8862753378503859
>1k	0.0017677360029282388	2.0402177240448482
>5k	3.9283022287294194E-4	1.5847431376651135
>10k+	5.89245334309413E-4	5.850232347572937
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	21868	2.4170773789720457	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG	16660	1.841435391150278	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	14583	1.6118638841023114	Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	8644	0.9554242209545621	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	5743	0.6347757173695107	Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	4179	0.4619062724860151	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2728	0.3015267555256878	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2672	0.29533705673190536	No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	1989	0.21984483751488013	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1754	0.19387020864811452	No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA	1732	0.19143854126484283	No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT	1392	0.15385822715973513	No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	1053	0.11638844339023066	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1023	0.11307253332213292	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	994	0.10986715358963844	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	952	0.1052248794943016	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.10530335603258E-4	0.0
3	0.0	0.0	0.0	1.10530335603258E-4	0.0
4	0.0	0.0	0.0	1.10530335603258E-4	0.0
5	0.0	0.0	0.0	1.10530335603258E-4	0.0
6	0.0	0.0	0.0	1.10530335603258E-4	0.0
7	0.0	0.0	0.0	1.10530335603258E-4	0.0
8	0.0	0.0	0.0	1.10530335603258E-4	0.0
9	0.0	0.0	0.0	3.31591006809774E-4	0.0
10	0.0	0.0	0.0	5.5265167801629E-4	0.0
11	0.0	0.0	0.0	5.5265167801629E-4	0.0
12	0.0	0.0	0.0	5.5265167801629E-4	0.0
13	0.0	0.0	0.0	6.63182013619548E-4	0.0
14	0.0	0.0	0.0	6.63182013619548E-4	0.0
15	0.0	0.0	0.0	6.63182013619548E-4	0.0
16	0.0	0.0	0.0	7.737123492228059E-4	0.0
17	0.0	0.0	0.0	7.737123492228059E-4	0.0
18	0.0	0.0	0.0	7.737123492228059E-4	0.0
19	0.0	0.0	0.0	7.737123492228059E-4	0.0
20	0.0	0.0	0.0	8.84242684826064E-4	0.0
21	0.0	1.10530335603258E-4	0.0	0.001326364027239096	0.0
22	0.0	1.10530335603258E-4	0.0	0.0015474246984456118	0.0
23	0.0	2.21060671206516E-4	0.0	0.0016579550340488699	0.0
24	0.0	2.21060671206516E-4	0.0	0.001879015705255386	0.0
25	0.0	2.21060671206516E-4	0.0	0.0021000763764619018	0.0
26	0.0	2.21060671206516E-4	0.0	0.002321137047668418	0.0
27	0.0	2.21060671206516E-4	0.0	0.0030948493968912237	0.0
28	0.0	2.21060671206516E-4	0.0	0.009174017855070413	0.0
29	0.0	2.21060671206516E-4	0.0	0.020669172757809245	0.0
30	0.0	2.21060671206516E-4	0.0	0.03503811638623278	0.0
31	0.0	2.21060671206516E-4	0.0	0.07593434055943823	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2440	0.0	34.043034	1
TTATACA	2690	0.0	30.672863	2
CGTCTTC	705	0.0	30.439716	37
TATACAC	2870	0.0	29.071428	3
TTACGGG	75	4.620233E-10	27.133333	3
TATAGCG	35	8.867931E-4	26.42857	5
GGTATCA	500	0.0	25.9	1
GATTACG	80	9.786163E-10	25.437502	1
CCGTCTT	2115	0.0	25.36643	37
GCCGTCT	2190	0.0	24.497717	36
CGTCAAT	55	1.902078E-5	23.545454	30
GTATCAA	1350	0.0	23.433332	1
CCGTAGA	40	0.0019308707	23.125002	2
TGCGGGT	60	3.725157E-5	21.583334	21
GCGGGTA	60	3.725157E-5	21.583334	22
TGCCGTC	2625	0.0	20.297144	35
CTCTATA	110	1.7535058E-9	20.181818	2
ATGCCGT	2730	0.0	19.584248	34
CGGTCCG	60	9.234743E-4	18.5	21
CTGTGCG	50	0.007033753	18.5	9
>>END_MODULE