FastQCFastQC Report
Fri 10 Feb 2017
ERR1630950.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630950.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588856
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT195173.3143926528726886No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG149992.547142255492005No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT146232.483289632779491TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC74131.2588816281060224No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC62721.065116089502357RNA PCR Primer, Index 3 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51240.8701618052630864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49790.8455377885255478No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC42640.7241159128887198No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30650.5205007675900389No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19680.33420734441017835No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16810.2854687733503607No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA16460.2795250451723341No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16310.27697773309603707No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG16100.27341149618922117No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13970.23723966470580246No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13160.22348417949379812No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT12680.21533278084964746No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12610.21414403521404216No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT12570.21346475199369624No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11990.20361514529868083No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11070.18799163123072532No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10820.1837461111035635No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT10030.17033026750173216No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG9650.1638770769084462No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8090.13738503131495647No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG7890.133988615213227No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7810.1326300487725352No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7540.12804488703520048No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6930.1176858179249256No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6770.11496868504354205No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6760.11479886423845559No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6700.11377993940793675No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6690.11361011860285028No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6320.10732674881465078No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6310.10715692800956432No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6280.1066474655943049No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.10647764478921842No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5900.10019427500101892No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAC200.001841207237.03
CGTAACT200.001841207237.027
GATTACG900.034.9444471
CTTATAC22150.034.2437931
CGTCTTC9000.033.91666837
TTATACA23150.032.6846662
GGTATCA6250.031.081
AGCAACG601.2678356E-930.8333322
ATTACTT303.5973662E-430.8333322
GCTCTAG303.5973662E-430.8333321
TATACAC25400.030.0078743
TGCTTAT250.00549419329.611
GAGTCAA250.00549419329.628
CTATACA502.71697E-729.61
TGTTGAA250.00549419329.630
CTTAGGA250.00549419329.62
GATAGTC250.00549419329.67
GCATCCG250.00549419329.637
TGCCGTA250.00549419329.624
GCCTTAC454.003892E-628.77777925
Produced by FastQC (version 0.11.5)