FastQCFastQC Report
Fri 10 Feb 2017
ERR1630949.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630949.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876852
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT147421.6812415322084No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG116061.323598509212501No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT100651.1478561946599881RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC62680.7148298686665481No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC33800.3854698398361411RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC30340.34601050120202725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23980.27347830648729776No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT21970.25055539589349174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21860.24930090824905457No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC21720.24770428761068003No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC18920.21577187484318905No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC18830.21474547586137682No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG18230.20790281598262877No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT18170.20721854999475398No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA18080.20619215101294175No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT18070.20607810668162926No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG16450.18760292500900952No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA14640.16696090104145284No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC14210.16205699479501673No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA13850.15795139886776788No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT13580.15487220192233123No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA12890.14700314306177098No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG12620.14392394611633433No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC12500.14255541414058473No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC11520.1313790696719629No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG11360.1295543603709634No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA11130.12693134075077664No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11100.12658920775683924No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT10920.12453640979321481No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG10330.11780779424577922No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA10310.11757970558315428No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG10090.11507073029427999No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA10010.11415837564378024No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC9780.11153535602359348No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA9610.10959660239128154No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA8770.10001687856103425No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGA200.001841697837.01
CTTATAC18100.032.502761
TTATACA18800.031.5877652
TATACAC19700.030.5203063
TCGCACG250.00549564229.627
GATTACG803.274181E-1127.7500021
CCGTCTT16500.026.46060837
CGTCTTC5050.026.37623837
GGTATCA4650.026.2580641
ATTACGG856.91216E-1126.1176452
GCCGTCT16850.025.80118636
GTATCAA13950.024.93191
TTACGGG901.4188117E-1024.6666663
GACGTTG400.001930823723.12500230
GTATAGG705.0996478E-621.1428571
TGCCGTC20850.020.85131835
AGCCGCG450.003825095220.55555519
CTTAGAC450.003825095220.5555553
GGCTAAC555.141756E-420.1818181
TCTTACA759.261663E-619.7333342
Produced by FastQC (version 0.11.5)