##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630949.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	876852
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51504472818674	31.0	31.0	34.0	28.0	34.0
2	31.521362784141452	31.0	31.0	34.0	30.0	34.0
3	30.99664253488616	31.0	31.0	34.0	27.0	34.0
4	35.14193159164831	37.0	35.0	37.0	32.0	37.0
5	35.33153827555847	37.0	35.0	37.0	33.0	37.0
6	35.51911611081459	37.0	35.0	37.0	33.0	37.0
7	35.52407817967	37.0	35.0	37.0	33.0	37.0
8	35.546722822095404	37.0	35.0	37.0	33.0	37.0
9	37.31726676793803	39.0	37.0	39.0	34.0	39.0
10	37.16740681437688	39.0	37.0	39.0	33.0	39.0
11	37.213972255295076	39.0	37.0	39.0	33.0	39.0
12	37.153870892693405	39.0	37.0	39.0	33.0	39.0
13	37.21969728072696	39.0	37.0	39.0	34.0	39.0
14	38.36904175391058	40.0	38.0	41.0	34.0	41.0
15	38.39324424190171	40.0	38.0	41.0	34.0	41.0
16	38.373816790062634	40.0	38.0	41.0	34.0	41.0
17	38.36289590489615	40.0	38.0	41.0	34.0	41.0
18	38.39413492812926	40.0	38.0	41.0	34.0	41.0
19	38.43801348460173	40.0	38.0	41.0	34.0	41.0
20	38.441094962433795	40.0	38.0	41.0	34.0	41.0
21	38.39065885691086	40.0	38.0	41.0	34.0	41.0
22	38.33617075629639	40.0	38.0	41.0	34.0	41.0
23	38.32448577411011	40.0	38.0	41.0	34.0	41.0
24	38.28497739641353	40.0	38.0	41.0	34.0	41.0
25	38.2538318895321	40.0	38.0	41.0	34.0	41.0
26	38.156699192109954	40.0	38.0	41.0	33.0	41.0
27	38.10079694178721	40.0	37.0	41.0	33.0	41.0
28	38.095179118026756	40.0	37.0	41.0	33.0	41.0
29	38.03768252795226	40.0	37.0	41.0	33.0	41.0
30	37.97902268569838	40.0	37.0	41.0	33.0	41.0
31	37.9613412525717	40.0	37.0	41.0	33.0	41.0
32	37.86323233567352	40.0	37.0	41.0	33.0	41.0
33	37.8314105459074	40.0	37.0	41.0	33.0	41.0
34	37.7858783466309	40.0	37.0	41.0	33.0	41.0
35	37.77588122054805	40.0	37.0	41.0	33.0	41.0
36	37.73345672929981	40.0	37.0	41.0	33.0	41.0
37	37.69528267027959	40.0	37.0	41.0	33.0	41.0
38	37.64471655421896	40.0	37.0	41.0	33.0	41.0
39	37.56509080209659	40.0	37.0	41.0	32.0	41.0
40	37.502612755630366	40.0	37.0	41.0	32.0	41.0
41	37.469360849949595	40.0	37.0	41.0	32.0	41.0
42	37.42964034979677	40.0	37.0	41.0	32.0	41.0
43	36.778821283409286	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	5.0
10	13.0
11	11.0
12	11.0
13	3.0
14	7.0
15	6.0
16	9.0
17	6.0
18	19.0
19	32.0
20	66.0
21	124.0
22	241.0
23	539.0
24	948.0
25	1593.0
26	2698.0
27	4301.0
28	6573.0
29	9682.0
30	13384.0
31	18269.0
32	24132.0
33	31443.0
34	41810.0
35	56597.0
36	79193.0
37	117377.0
38	207834.0
39	259921.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.13950358783466	22.433546368144224	15.34466477809254	24.082285265928572
2	13.44571261740864	22.48041858831365	41.631883145616364	22.441985648661348
3	14.498569884085342	28.414373235163975	35.69154201621254	21.395514864538143
4	11.96416270932837	19.4248288194587	37.37289759275225	31.238110878460674
5	12.119719177238576	39.007038816128606	35.783689835913016	13.0895521707198
6	27.271192858087794	39.948930948438274	18.185509071086113	14.594367122387814
7	23.249191425690995	34.22709875782915	23.070712047186984	19.452997769292878
8	23.810631668742275	36.379913600014596	22.297263392225826	17.512191339017303
9	23.35890207241359	15.898007873620635	19.42494286379001	41.318147190175765
10	13.861746338036522	29.599521926163135	33.89637019702298	22.642361538777354
11	30.473329592679267	23.49199180705524	25.985799199864974	20.048879400400523
12	19.90278861198925	27.113013370557404	29.18827806745038	23.795919950002965
13	27.633283610004884	21.312946768667917	27.951353250035353	23.10241637129185
14	21.187383959892887	23.072194623494045	26.644633301857095	29.095788114755965
15	23.648802762609883	29.16615346717576	23.815421530657396	23.36962223955696
16	24.319155342064565	28.44128769735372	23.460857704606937	23.778699255974782
17	21.078015446164233	30.866212314050717	24.836802561891858	23.21896967789319
18	22.513605488725577	26.777038770510874	26.318238425640818	24.391117315122735
19	23.613905197228267	27.440548690086807	26.46877694297327	22.47676916971165
20	22.63392225826023	25.71939164191905	27.196835954072068	24.449850145748655
21	22.574391117315123	27.241199198952614	26.679758955901338	23.50465072783092
22	19.993453855382665	32.4896333702837	25.73512975964017	21.78178301469347
23	22.059595005770642	28.08615364964669	26.403315496799916	23.450935847782752
24	24.796430868607246	28.644970873077785	25.354677870381774	21.2039203879332
25	21.795354290119658	29.159766984622266	26.045558429472702	22.999320295785378
26	23.13902460164315	26.562065205986872	26.27364709209764	24.025263100272337
27	20.747058796695452	29.978377194783157	26.487936390633767	22.786627617887625
28	19.8245542007089	27.47099852654724	29.023484008703864	23.68096326404
29	21.873930834393946	28.64223380912628	25.86981611492019	23.61401924155958
30	20.120043063139505	29.34588733332421	27.434162207533312	23.099907396002976
31	23.368709884906462	26.352793858028495	27.72794040499423	22.550555852070815
32	21.376697549871587	30.398402467006974	27.060210845159734	21.16468913796171
33	20.725504418077396	27.98807552471797	28.404679467002413	22.881740590202224
34	22.17774493301036	28.936354139581137	26.443459101421908	22.4424418259866
35	21.231861249104753	28.433076505499216	29.158512496977824	21.176549748418203
36	22.42179980201904	27.00478530014187	25.30871800486285	25.264696892976236
37	22.782978199285626	27.013338624990308	27.384666967743705	22.819016207980365
38	22.396139827473736	26.061524635856447	28.283336298485946	23.25899923818387
39	23.598509212501085	26.18857002093854	27.122479050056334	23.09044171650404
40	21.335983723593035	25.377258647981645	31.016408698389235	22.270348930036082
41	21.858306761004137	27.053938406937544	26.906935263875774	24.180819568182542
42	23.12739207984928	25.386724327480582	29.24552832176924	22.240355270900906
43	22.316080706892382	25.43770214357725	29.324675087700093	22.921542061830273
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	13.5
2	20.0
3	81.5
4	143.0
5	143.0
6	184.5
7	226.0
8	243.5
9	261.0
10	392.5
11	524.0
12	524.0
13	795.0
14	1066.0
15	2813.5
16	4561.0
17	5178.0
18	5795.0
19	5795.0
20	6277.0
21	6759.0
22	8572.0
23	10385.0
24	12807.5
25	15230.0
26	15230.0
27	18745.0
28	22260.0
29	29173.5
30	36087.0
31	37437.0
32	38787.0
33	38787.0
34	42432.0
35	46077.0
36	47808.0
37	49539.0
38	55378.0
39	61217.0
40	61217.0
41	65501.5
42	69786.0
43	63411.5
44	57037.0
45	59297.0
46	61557.0
47	61557.0
48	63357.5
49	65158.0
50	78463.0
51	91768.0
52	79171.5
53	66575.0
54	66575.0
55	63773.5
56	60972.0
57	50802.0
58	40632.0
59	31048.5
60	21465.0
61	21465.0
62	18536.0
63	15607.0
64	12967.0
65	10327.0
66	8611.5
67	6896.0
68	6896.0
69	5572.0
70	4248.0
71	3513.0
72	2778.0
73	2170.5
74	1563.0
75	1563.0
76	1176.5
77	790.0
78	590.0
79	390.0
80	285.5
81	181.0
82	181.0
83	137.5
84	94.0
85	73.0
86	52.0
87	39.5
88	27.0
89	27.0
90	16.0
91	5.0
92	2.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	876852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.44299962402493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.27812169722714	35.74408409958116
2	10.522355765954652	9.14245395168449
3	2.7729474918917285	3.613954705430797
4	1.163177230986137	2.021276320336205
5	0.6297044149889823	1.36781243318066
6	0.4188473785374945	1.091759190499692
7	0.28952979188152955	0.8804629847897313
8	0.22415730058767686	0.779045242012231
9	0.16294165272885192	0.6370806742413763
>10	1.1921371305808506	10.375625814295935
>50	0.1605010124660565	4.903557766008174
>100	0.16234874293586007	14.911929491973163
>500	0.014518993270665463	4.229226832511117
>1k	0.007655469179078153	5.407006526905855
>5k	2.639816958302812E-4	0.7188240627523098
>10k+	7.919450874908435E-4	4.175899903797042
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	14742	1.6812415322084	No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG	11606	1.323598509212501	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	10065	1.1478561946599881	RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC	6268	0.7148298686665481	No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	3380	0.3854698398361411	RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	3034	0.34601050120202725	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2398	0.27347830648729776	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2197	0.25055539589349174	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2186	0.24930090824905457	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2172	0.24770428761068003	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1892	0.21577187484318905	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1883	0.21474547586137682	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1823	0.20790281598262877	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1817	0.20721854999475398	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1808	0.20619215101294175	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1807	0.20607810668162926	No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG	1645	0.18760292500900952	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1464	0.16696090104145284	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1421	0.16205699479501673	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1385	0.15795139886776788	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1358	0.15487220192233123	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1289	0.14700314306177098	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1262	0.14392394611633433	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1250	0.14255541414058473	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	1152	0.1313790696719629	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	1136	0.1295543603709634	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	1113	0.12693134075077664	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1110	0.12658920775683924	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	1092	0.12453640979321481	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	1033	0.11780779424577922	No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA	1031	0.11757970558315428	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	1009	0.11507073029427999	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	1001	0.11415837564378024	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	978	0.11153535602359348	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	961	0.10959660239128154	No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA	877	0.10001687856103425	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.1404433131246778E-4	0.0
11	0.0	0.0	0.0	2.2808866262493557E-4	0.0
12	0.0	0.0	0.0	2.2808866262493557E-4	0.0
13	0.0	0.0	0.0	3.4213299393740334E-4	0.0
14	0.0	0.0	0.0	3.4213299393740334E-4	0.0
15	0.0	0.0	0.0	3.4213299393740334E-4	0.0
16	0.0	0.0	0.0	4.5617732524987114E-4	0.0
17	0.0	0.0	0.0	4.5617732524987114E-4	0.0
18	0.0	0.0	0.0	5.702216565623389E-4	0.0
19	0.0	0.0	0.0	6.842659878748067E-4	0.0
20	0.0	0.0	0.0	7.983103191872745E-4	0.0
21	0.0	0.0	0.0	0.0012544876444371457	0.0
22	0.0	0.0	0.0	0.001596620638374549	0.0
23	0.0	0.0	0.0	0.0019387536323119522	0.0
24	0.0	0.0	0.0	0.0022808866262493555	0.0
25	0.0	0.0	0.0	0.002623019620186759	0.0
26	0.0	0.0	0.0	0.00307919694543663	0.0
27	0.0	0.0	0.0	0.00410559592724884	0.0
28	0.0	0.0	0.0	0.009693768161559762	0.0
29	0.0	0.0	0.0	0.02155437861805641	0.0
30	0.0	0.0	0.0	0.03740654067048943	0.0
31	0.0	0.0	0.0	0.08450684950253863	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGA	20	0.0018416978	37.0	1
CTTATAC	1810	0.0	32.50276	1
TTATACA	1880	0.0	31.587765	2
TATACAC	1970	0.0	30.520306	3
TCGCACG	25	0.005495642	29.6	27
GATTACG	80	3.274181E-11	27.750002	1
CCGTCTT	1650	0.0	26.460608	37
CGTCTTC	505	0.0	26.376238	37
GGTATCA	465	0.0	26.258064	1
ATTACGG	85	6.91216E-11	26.117645	2
GCCGTCT	1685	0.0	25.801186	36
GTATCAA	1395	0.0	24.9319	1
TTACGGG	90	1.4188117E-10	24.666666	3
GACGTTG	40	0.0019308237	23.125002	30
GTATAGG	70	5.0996478E-6	21.142857	1
TGCCGTC	2085	0.0	20.851318	35
AGCCGCG	45	0.0038250952	20.555555	19
CTTAGAC	45	0.0038250952	20.555555	3
GGCTAAC	55	5.141756E-4	20.181818	1
TCTTACA	75	9.261663E-6	19.733334	2
>>END_MODULE