FastQCFastQC Report
Fri 10 Feb 2017
ERR1630948.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630948.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312281
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT176145.6404328153169745No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG140784.508119289998431No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT128654.119687076703354RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC70462.256301215892097No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC53021.6978298391512774RNA PCR Primer, Index 33 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52111.6686894175438147No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48741.560773790272223No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC41671.334375130091168No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA32091.0276001421796395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19740.6321229917926483No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA18080.5789657391900244No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16600.5315725260262392No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG15930.5101174903372283No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA14440.46240405276017427No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14410.46144337952036785No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13510.43262318232617414No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13060.41821308372907734No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12930.4140501663565827No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11950.38266817385623847No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11860.3797861541368191No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11810.378185032070475No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT11790.37754458324393736No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG9340.2990896019930767No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT9220.2952469090338509No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8980.2875615231153993No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG8270.2648255897733131No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8190.2622637944671626No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7950.25457840854871094No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7930.2539379597221733No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7640.24465145173737757No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7510.24048853436488293No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7490.23984808553834525No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7140.2286402310739366No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG7050.22575821135451726No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6920.22159529398202263No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6490.2078256442114634No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6250.20014025829301177No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5880.18829195500206544No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5650.18092679349688262No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5620.17996612025707615No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5590.17900544701726973No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5570.17836499819073207No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5530.1770841005376568No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5510.17644365171111917No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5360.17164028551208688No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5290.16939871461920514No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5160.1652357972467105No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT4830.15466839160883947No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4760.15242682071595776No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4750.1521065963026889No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4630.1482639033434631No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4620.14794367893019428No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4560.14602233245058135No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4470.14314031273116198No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4410.1412189662515491No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4260.1364156000525168No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4190.13417402915963508No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG4110.13161223385348453No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4090.1309717850269469No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4070.13033133620040924No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT4070.13033133620040924No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4050.12969088737387163No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4050.12969088737387163No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3900.12488752117483934No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3880.12424707234830168No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3840.12296617469522642No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC3810.12200550145541995No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3790.12136505262888232No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3730.1194437061492694No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3700.11848303290946297No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3690.11816280849619412No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3630.11624146201658123No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3610.11560101319004357No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3570.1143201155369683No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3550.11367966671043067No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3510.1123987690573554No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3440.11015719816447365No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG3410.1091965249246672No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3370.10791562727159193No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3330.10663472961851665No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3330.10663472961851665No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG3270.10471338313890374No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA3240.10375270989909728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3210.10279203665929082No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3160.10119091459294674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCAA251.2315673E-437.030
GACAGAC403.8180588E-837.016
ATGATCA200.001839883637.034
CAATCTG200.001839883637.023
ACTCAGA750.037.01
TCAGAGT750.037.03
GATTACG600.037.01
TTACGGG600.037.03
CGCCGAG308.292596E-637.02
ACTATGT200.001839883637.03
CTCGAGC200.001839883637.012
CTTATAC20750.035.0385551
CGTCTTC6000.034.53333337
ATTACGG651.8189894E-1234.1538472
TGTGTAT507.2959665E-933.3000035
CAGACAC451.0756776E-732.8888918
TTTACAC451.0756776E-732.888892
AGACAGA451.0756776E-732.8888915
TTATACA22200.032.752
GTTTGTG352.3827813E-531.7142851
Produced by FastQC (version 0.11.5)