FastQCFastQC Report
Fri 10 Feb 2017
ERR1630947.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630947.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863090
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT165451.9169495649352906No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG138241.601686961962252No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT104071.2057838695848637TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC76660.8882040111691712No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC35900.4159473519563429Illumina Paired End PCR Primer 2 (95% over 23bp)
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC35300.40899558562838173No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25860.29962112873512614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24050.27864996697910993No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC22550.261270551159207No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG18540.21480957953399993No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13400.15525611465779932No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA13040.1510850548610226No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG10700.12397316618197407No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTAT10280.11910692975240125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8850.10253855333742716No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT8820.10219096502102909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC21800.032.756881
AGCAACG401.5994556E-632.3752
TTATACA22250.032.177532
ACGGTGT303.598743E-430.83333235
TATACAC23850.030.4067083
GGACGTA250.005495594329.627
CGTCTTC5200.028.1057737
GATTACG604.3202817E-827.7499981
TTACCGG358.867597E-426.42857231
CCGTCTT16500.025.45151537
GTATCAA14850.025.2895621
GCCGTCT16650.025.1111136
GGTATCA4900.023.4081651
ACGTATT400.001930799723.12516
CCGTTTA400.001930799723.12527
GCAACGC705.099493E-621.1428593
GACCGTT705.099493E-621.1428597
TGCCGTC19900.020.91708635
CGAAAGT450.003825046820.55555720
CGCCGTT450.003825046820.55555725
Produced by FastQC (version 0.11.5)