##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630947.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	863090
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45423652226303	31.0	31.0	34.0	28.0	34.0
2	31.451425691411092	31.0	31.0	34.0	28.0	34.0
3	30.90996419840341	31.0	31.0	34.0	27.0	34.0
4	35.06822926925349	37.0	35.0	37.0	32.0	37.0
5	35.27025107462721	37.0	35.0	37.0	33.0	37.0
6	35.458703032128746	37.0	35.0	37.0	33.0	37.0
7	35.46224959158373	37.0	35.0	37.0	33.0	37.0
8	35.49179228122212	37.0	35.0	37.0	33.0	37.0
9	37.265993117751336	39.0	37.0	39.0	34.0	39.0
10	37.09543384815025	39.0	37.0	39.0	33.0	39.0
11	37.1562884519575	39.0	37.0	39.0	33.0	39.0
12	37.09310384780266	39.0	37.0	39.0	33.0	39.0
13	37.17165301417002	39.0	37.0	39.0	33.0	39.0
14	38.28822254921271	40.0	38.0	41.0	33.0	41.0
15	38.3001355594434	40.0	38.0	41.0	33.0	41.0
16	38.286834513202564	40.0	38.0	41.0	33.0	41.0
17	38.25750269380945	40.0	38.0	41.0	33.0	41.0
18	38.287944478559595	40.0	38.0	41.0	33.0	41.0
19	38.30830620213419	40.0	38.0	41.0	34.0	41.0
20	38.323326651913476	40.0	38.0	41.0	34.0	41.0
21	38.26898237727236	40.0	38.0	41.0	34.0	41.0
22	38.198282913716994	40.0	38.0	41.0	34.0	41.0
23	38.19630281894125	40.0	38.0	41.0	34.0	41.0
24	38.14263981740027	40.0	37.0	41.0	33.0	41.0
25	38.10966758970675	40.0	37.0	41.0	33.0	41.0
26	37.991478293109644	40.0	37.0	41.0	33.0	41.0
27	37.91472731696579	40.0	37.0	41.0	33.0	41.0
28	37.90194301868866	40.0	37.0	41.0	33.0	41.0
29	37.824279043900404	40.0	37.0	41.0	33.0	41.0
30	37.73627199944386	40.0	37.0	41.0	33.0	41.0
31	37.757481838510465	40.0	37.0	41.0	33.0	41.0
32	37.612035824769144	40.0	37.0	41.0	32.0	41.0
33	37.58990835254724	40.0	37.0	41.0	32.0	41.0
34	37.51281673985332	40.0	37.0	41.0	32.0	41.0
35	37.44833447265059	39.0	36.0	41.0	32.0	41.0
36	37.45888030217011	39.0	37.0	41.0	32.0	41.0
37	37.36453208819474	39.0	36.0	41.0	31.0	41.0
38	37.33169310268917	39.0	36.0	41.0	31.0	41.0
39	37.269408752273804	39.0	36.0	41.0	31.0	41.0
40	37.172560219675816	39.0	36.0	41.0	31.0	41.0
41	37.12115770081915	39.0	36.0	41.0	31.0	41.0
42	37.074856619819485	39.0	36.0	41.0	31.0	41.0
43	36.38661669119095	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	17.0
11	21.0
12	10.0
13	13.0
14	10.0
15	8.0
16	8.0
17	10.0
18	12.0
19	34.0
20	78.0
21	174.0
22	351.0
23	656.0
24	1113.0
25	1899.0
26	3112.0
27	4821.0
28	7389.0
29	10516.0
30	14543.0
31	19136.0
32	25438.0
33	33103.0
34	43841.0
35	59481.0
36	81208.0
37	119900.0
38	208709.0
39	227475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.05095644718396	21.61373669026405	15.017553209978102	25.31775365257389
2	14.21427661078219	21.2055521440406	40.95192853584215	23.628242709335062
3	14.713992746990465	28.198102167792467	33.53960768865356	23.548297396563513
4	11.968624361306468	18.959668169020613	36.9451621499496	32.12654531972332
5	12.848486252882086	38.00727618208993	34.96275011875934	14.18148744626864
6	28.43399877185461	39.05479150494155	17.453104542979293	15.058105180224542
7	25.23039312238585	32.43195958706508	22.134887439316874	20.202759851232198
8	25.257620873837027	34.45654566731164	22.082401603540767	18.20343185531057
9	24.34659189655772	14.629760511650003	18.92201276807749	42.10163482371479
10	15.236881437625277	28.11618718789466	32.703426062171964	23.9435053123081
11	31.870372730537948	22.6372684192842	24.79185252986363	20.70050632031422
12	21.485013150424635	25.705662213674124	28.22926925349616	24.58005538240508
13	29.135200268801633	19.870465420755657	26.510560891679898	24.483773418762816
14	21.877092771321646	21.793439849841846	25.875401174848513	30.454066203987995
15	24.524325389009256	27.703252268013767	22.425702997369914	25.34671934560706
16	24.99287443951384	26.277560856921063	22.92495568248966	25.804609021075436
17	21.286076770672814	29.973003974093082	24.323187616586914	24.417731638647187
18	24.309052358386726	25.075484596044443	25.18358456244424	25.43187848312459
19	24.234089144816878	27.50929798746365	24.805987788063817	23.45062507965566
20	22.769931293376125	25.314857083270574	25.37162984161559	26.543581781737707
21	25.755599068463315	25.12055521440406	25.76452050191753	23.359325215215097
22	23.23685826507085	29.54744001204973	24.91478293109641	22.300918791783012
23	24.160168696196223	25.923947676372105	25.39827827920611	24.517605348225562
24	25.424347402935965	27.347321832022153	24.601953446338158	22.626377318703728
25	22.759503643884184	26.830805593854638	25.27152440649295	25.138166355768227
26	25.923947676372105	26.88143762527662	25.96507896047921	21.229535737872066
27	23.335573346927898	27.708118504443334	24.863339860269495	24.092968288359266
28	22.097463763918014	25.481699475141646	28.347681006615765	24.073155754324578
29	25.11105446708918	26.511140205540556	25.395613435447057	22.982191891923208
30	22.232559756224727	26.11384675989758	26.877382428251977	24.776211055625716
31	25.68863038617062	24.186932996558877	27.013984636596415	23.11045198067409
32	24.500457657949923	25.323430928408392	26.2649318147586	23.911179598883084
33	23.677020936402922	27.104241736087776	27.03090060132779	22.187836726181512
34	24.37602104067942	27.24976537788643	25.542875018827697	22.831338562606447
35	22.779547903463136	24.686880858311415	29.251410629250717	23.28216060897473
36	26.108517072379474	25.11580484074662	24.159705245107695	24.61597284176621
37	22.992503678643015	26.296330626006558	27.23597770800264	23.475187987347784
38	23.33951268118041	24.37393551078103	27.48670474689777	24.799847061140785
39	24.371618255338376	25.07131353624767	26.04502427325076	24.51204393516319
40	22.17057317313374	23.96065300258374	31.12340543859852	22.745368385683996
41	23.118098923634847	25.00110069633526	26.0735265151954	25.80727386483449
42	23.287837884809232	24.6180583716646	28.745322040575143	23.348781702951023
43	23.981508301567622	23.38006465142685	28.95468606981891	23.68374097718662
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	10.0
2	14.0
3	69.0
4	124.0
5	124.0
6	149.0
7	174.0
8	184.5
9	195.0
10	337.5
11	480.0
12	480.0
13	729.0
14	978.0
15	2446.0
16	3914.0
17	4383.5
18	4853.0
19	4853.0
20	4745.0
21	4637.0
22	5353.5
23	6070.0
24	8130.5
25	10191.0
26	10191.0
27	12903.5
28	15616.0
29	19209.5
30	22803.0
31	26957.0
32	31111.0
33	31111.0
34	35601.0
35	40091.0
36	43787.0
37	47483.0
38	50761.0
39	54039.0
40	54039.0
41	56529.5
42	59020.0
43	60009.5
44	60999.0
45	61358.0
46	61717.0
47	61717.0
48	60884.5
49	60052.0
50	60067.0
51	60082.0
52	73016.0
53	85950.0
54	85950.0
55	70392.5
56	54835.0
57	58724.5
58	62614.0
59	50981.5
60	39349.0
61	39349.0
62	32537.5
63	25726.0
64	20349.0
65	14972.0
66	12199.5
67	9427.0
68	9427.0
69	7975.5
70	6524.0
71	5644.0
72	4764.0
73	5760.0
74	6756.0
75	6756.0
76	5876.5
77	4997.0
78	3455.5
79	1914.0
80	1135.5
81	357.0
82	357.0
83	243.5
84	130.0
85	101.0
86	72.0
87	54.0
88	36.0
89	36.0
90	23.5
91	11.0
92	8.5
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	863090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.76537343502746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.54652233339627	38.47334718147452
2	11.449651153624512	10.937937261073355
3	3.1951841794440266	4.578574965745072
4	1.312393735381668	2.5074790745718394
5	0.6972146584622432	1.6651359262912084
6	0.45940683837703294	1.3166243516210572
7	0.31579974820060736	1.0559005032442756
8	0.21756090195769823	0.8313502181496683
9	0.19158719874515104	0.823611068408967
>10	1.3346490521308612	12.948595896475732
>50	0.16903846137222553	5.605521244123153
>100	0.10079118522303097	8.961263649250139
>500	0.0068003691231424464	2.2432451121818078
>1k	0.002428703258265159	2.431757979084401
>5k	2.4287032582651593E-4	0.8893167001078627
>10k+	7.286109774795478E-4	4.730338868197001
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT	16545	1.9169495649352906	No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG	13824	1.601686961962252	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	10407	1.2057838695848637	TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC	7666	0.8882040111691712	No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC	3590	0.4159473519563429	Illumina Paired End PCR Primer 2 (95% over 23bp)
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	3530	0.40899558562838173	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2586	0.29962112873512614	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2405	0.27864996697910993	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2255	0.261270551159207	No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG	1854	0.21480957953399993	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1340	0.15525611465779932	No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA	1304	0.1510850548610226	No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG	1070	0.12397316618197407	No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTAT	1028	0.11910692975240125	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	885	0.10253855333742716	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	882	0.10219096502102909	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.4758831639805816E-4	0.0	0.0	0.0	0.0
6	3.4758831639805816E-4	0.0	0.0	0.0	0.0
7	3.4758831639805816E-4	0.0	0.0	0.0	0.0
8	4.634510885307442E-4	0.0	0.0	0.0	0.0
9	4.634510885307442E-4	0.0	0.0	0.0	0.0
10	4.634510885307442E-4	0.0	0.0	0.0	0.0
11	4.634510885307442E-4	0.0	0.0	0.0	0.0
12	4.634510885307442E-4	0.0	0.0	2.317255442653721E-4	0.0
13	4.634510885307442E-4	0.0	0.0	2.317255442653721E-4	0.0
14	4.634510885307442E-4	0.0	0.0	2.317255442653721E-4	0.0
15	5.793138606634302E-4	0.0	0.0	2.317255442653721E-4	0.0
16	5.793138606634302E-4	0.0	0.0	2.317255442653721E-4	0.0
17	0.0010427649491941745	0.0	0.0	3.4758831639805816E-4	0.0
18	0.0010427649491941745	0.0	0.0	4.634510885307442E-4	0.0
19	0.0010427649491941745	0.0	0.0	4.634510885307442E-4	0.0
20	0.0012744904934595465	0.0	0.0	5.793138606634302E-4	0.0
21	0.0012744904934595465	0.0	0.0	0.0010427649491941745	0.0
22	0.0013903532655922327	0.0	0.0	0.0018538043541229767	0.0
23	0.0013903532655922327	0.0	0.0	0.002433118214786407	0.0
24	0.0013903532655922327	0.0	0.0	0.0032441576197152094	0.0
25	0.0013903532655922327	0.0	0.0	0.003475883163980581	0.0
26	0.0013903532655922327	0.0	0.0	0.0037076087082459535	0.0
27	0.0016220788098576047	0.0	0.0	0.00440278534104207	0.0
28	0.0018538043541229767	0.0	0.0	0.009848335631278314	0.0
29	0.0018538043541229767	0.0	0.0	0.02259324056587378	0.0
30	0.0018538043541229767	0.0	0.0	0.039161616980847885	0.0
31	0.0018538043541229767	0.0	0.0	0.08573845137818767	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2180	0.0	32.75688	1
AGCAACG	40	1.5994556E-6	32.375	2
TTATACA	2225	0.0	32.17753	2
ACGGTGT	30	3.598743E-4	30.833332	35
TATACAC	2385	0.0	30.406708	3
GGACGTA	25	0.0054955943	29.6	27
CGTCTTC	520	0.0	28.10577	37
GATTACG	60	4.3202817E-8	27.749998	1
TTACCGG	35	8.867597E-4	26.428572	31
CCGTCTT	1650	0.0	25.451515	37
GTATCAA	1485	0.0	25.289562	1
GCCGTCT	1665	0.0	25.11111	36
GGTATCA	490	0.0	23.408165	1
ACGTATT	40	0.0019307997	23.125	16
CCGTTTA	40	0.0019307997	23.125	27
GCAACGC	70	5.099493E-6	21.142859	3
GACCGTT	70	5.099493E-6	21.142859	7
TGCCGTC	1990	0.0	20.917086	35
CGAAAGT	45	0.0038250468	20.555557	20
CGCCGTT	45	0.0038250468	20.555557	25
>>END_MODULE