FastQCFastQC Report
Fri 10 Feb 2017
ERR1630946.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630946.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16376
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT274116.73790913531998No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT182011.11382510991695TruSeq Adapter, Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG180311.010014655593551No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC9465.776746458231559No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC4923.0043966780654614RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC4262.6013678553981436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2021.2335124572545189No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1911.1663409868099657No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG1590.970933072789448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1430.873229115779189No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA1340.8182706399609184No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT1200.7327796775769418No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT970.5923302393746946No Hit
CTGATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT450.2747923790913532No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA450.2747923790913532No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC430.26257938446507084No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA410.25036638983878845No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT370.22594040058622372No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.21372740595994139No Hit
TCGCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT260.15876893014167073TruSeq Adapter, Index 10 (95% over 21bp)
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA230.1404494382022472No Hit
CTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA220.134342940889106No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT180.10991695163654128No Hit
CTTATAACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCG180.10991695163654128No Hit
TTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG180.10991695163654128No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA180.10991695163654128No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT170.1038104543234001TruSeq Adapter, Index 10 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC2750.036.3272741
TTATACA2850.035.0526352
TATACAC2950.033.8644073
CCGTCTT2950.027.5932237
GCCGTCT3050.026.68852436
CGTCTTC705.638867E-926.4285737
GGTATCA601.2080545E-624.6666661
TGCCGTC3550.022.92957735
ATGCCGT3700.022.034
ATACACA4900.021.5204094
TACACAT5050.020.881195
TATGCCG3950.020.60759533
AGTACTT1001.07338565E-820.34999812
GAGTACT1001.07338565E-820.34999811
GTACTTT1155.343645E-817.69565213
ACACATC6000.017.5756
ACTTTTT1208.69386E-816.95833215
TACTTTT1208.69386E-816.95833214
TATCAAC1755.456968E-1216.9142861
CACATCT6250.016.8727
Produced by FastQC (version 0.11.5)