##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630946.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16376
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.840254030288225	31.0	30.0	34.0	27.0	34.0
2	31.044272105520275	31.0	31.0	34.0	28.0	34.0
3	30.562408402540303	31.0	30.0	34.0	26.0	34.0
4	34.59910845139228	35.0	35.0	37.0	32.0	37.0
5	34.72099413776258	35.0	35.0	37.0	32.0	37.0
6	34.919821690278454	35.0	35.0	37.0	32.0	37.0
7	34.8580850024426	35.0	35.0	37.0	32.0	37.0
8	34.88202247191011	35.0	35.0	37.0	32.0	37.0
9	36.77729604298974	39.0	35.0	39.0	32.0	39.0
10	36.51776990718124	38.0	35.0	39.0	32.0	39.0
11	36.5180752320469	38.0	35.0	39.0	32.0	39.0
12	36.357840742550074	38.0	35.0	39.0	32.0	39.0
13	36.588299951148024	39.0	35.0	39.0	32.0	39.0
14	37.13135075720567	39.0	36.0	40.0	31.0	41.0
15	37.179408891060085	39.0	36.0	40.0	31.0	41.0
16	37.03737176355642	39.0	36.0	40.0	31.0	41.0
17	36.99041279921837	39.0	36.0	40.0	31.0	41.0
18	37.16053981436248	39.0	36.0	40.0	31.0	41.0
19	37.0445163654128	39.0	36.0	40.0	31.0	41.0
20	37.19974352711285	39.0	36.0	40.0	31.0	41.0
21	36.86584025403029	39.0	36.0	40.0	30.0	41.0
22	36.831827063996094	38.0	35.0	40.0	31.0	41.0
23	36.90834147532975	39.0	36.0	40.0	31.0	41.0
24	36.85814606741573	39.0	35.0	40.0	30.0	41.0
25	36.71910112359551	38.0	35.0	40.0	30.0	41.0
26	36.380007327796775	38.0	35.0	40.0	30.0	41.0
27	35.99969467513434	38.0	34.0	40.0	29.0	41.0
28	35.897349780166095	38.0	34.0	40.0	28.0	41.0
29	35.90534929164631	38.0	34.0	40.0	28.0	41.0
30	35.82156814851002	38.0	34.0	40.0	28.0	41.0
31	35.454201270151444	38.0	34.0	40.0	27.0	41.0
32	35.16786761113825	38.0	33.0	40.0	27.0	41.0
33	34.713361016121155	37.0	33.0	40.0	26.0	41.0
34	34.57132388861749	37.0	33.0	40.0	26.0	41.0
35	34.89960918417196	38.0	33.0	40.0	26.0	41.0
36	35.08207132388862	38.0	34.0	40.0	26.0	41.0
37	34.51398387884709	37.0	33.0	40.0	24.0	41.0
38	34.54079140205178	38.0	33.0	40.0	24.0	41.0
39	34.23357352222765	37.0	32.0	40.0	23.0	41.0
40	33.443820224719104	36.0	31.0	39.0	21.0	41.0
41	33.53419638495359	36.0	31.0	39.0	21.0	41.0
42	33.33463605276014	36.0	31.0	39.0	21.0	40.0
43	31.484306301905228	35.0	29.0	37.0	18.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	2.0
13	1.0
14	2.0
15	2.0
16	6.0
17	4.0
18	2.0
19	6.0
20	9.0
21	14.0
22	19.0
23	46.0
24	78.0
25	135.0
26	220.0
27	316.0
28	382.0
29	460.0
30	587.0
31	701.0
32	829.0
33	937.0
34	1261.0
35	1491.0
36	1765.0
37	2426.0
38	3031.0
39	1640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.278212017586714	25.87322911577919	21.51929653150953	33.32926233512457
2	6.8026380068392776	15.96238397655105	49.7923790913532	27.442598925256473
3	8.408646800195408	31.57059110893991	48.69320957498779	11.327552515876892
4	4.756961406936981	28.30361504640938	15.107474352711284	51.83194919394235
5	8.738397655105032	35.88788470933073	34.53224230581339	20.841475329750857
6	23.15583781143136	38.01294577430386	12.469467513434294	26.361748900830484
7	13.281631656082071	39.417440156326336	8.927699071812409	38.37322911577919
8	27.29604298974109	30.874450415241817	26.264044943820224	15.565461651196873
9	11.101612115290669	8.866634098680997	8.549096238397654	71.48265754763068
10	10.869565217391305	31.83317049340498	24.0657059110894	33.231558378114315
11	19.650708353688323	13.751831949193944	27.84562774792379	38.75183194919394
12	10.460429897410844	31.70493404982902	11.803859306301904	46.03077674645823
13	35.47264289203713	8.952125061064974	28.645578895945285	26.929653150952614
14	22.905471421592573	27.52198339032731	13.000732779677577	36.571812408402536
15	28.26086956521739	25.134342940889105	10.74132877381534	35.863458720078164
16	42.62335124572545	16.133365901319003	10.771861260381046	30.4714215925745
17	11.126038104543234	50.946507083536886	10.686370297997069	27.241084513922814
18	31.77210552027357	9.66047874938935	12.54885197850513	46.01856375183195
19	21.44601856375183	25.21983390327308	13.281631656082071	40.05251587689301
20	23.479482169027847	20.035417684416217	13.208353688324378	43.27674645823156
21	31.60112359550562	24.07181240840254	13.562530532486566	30.764533463605275
22	27.51587689301417	47.64899853444064	13.519785051294578	11.31533952125061
23	34.75207620908647	13.391548607718612	14.612848070346848	37.24352711284807
24	31.008793356130926	24.059599413776258	17.006595017098192	27.925012212994627
25	22.429164631167563	31.308011724474845	26.612115290669276	19.650708353688323
26	40.32730825598437	21.281143136297022	15.003663898387885	23.387884709330727
27	28.242550073277968	40.49829018075232	20.5666829506595	10.69247679531021
28	16.62799218368344	23.100879335613094	28.126526624328285	32.14460185637518
29	22.020029311187102	13.935026868588176	18.404982901807525	45.639960918417195
30	40.33341475329751	12.371763556424035	35.64973131411822	11.645090376160235
31	21.250610649731314	29.219589643380555	15.113580850024427	34.4162188568637
32	39.27699071812408	17.018808011724477	33.585735222276504	10.118466047874938
33	39.417440156326336	13.623595505617978	34.4894968246214	12.469467513434294
34	11.461895456765998	34.4162188568637	20.407914020517833	33.71397166585247
35	12.365657059110893	11.755007327796775	66.00512945774304	9.874206155349292
36	39.25867122618466	15.052515876893013	15.559355153883732	30.129457743038596
37	14.881533952125062	29.683683439179287	30.80117244748412	24.63361016121153
38	13.898387884709331	11.77943331704934	39.52735710796287	34.794821690278454
39	32.45603321934538	16.585246702491453	19.956033219345386	31.00268685881778
40	9.782608695652174	11.010014655593551	62.39618954567659	16.811187103077675
41	19.174401563263313	21.910112359550563	18.58817782120176	40.32730825598437
42	26.526624328285298	11.72447484123107	44.89496824621397	16.853932584269664
43	20.908646800195406	12.49389350268686	45.06595017098192	21.53150952613581
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	6.5
4	13.0
5	13.0
6	21.5
7	30.0
8	29.5
9	29.0
10	48.5
11	68.0
12	68.0
13	88.0
14	108.0
15	209.0
16	310.0
17	320.5
18	331.0
19	331.0
20	309.0
21	287.0
22	212.5
23	138.0
24	120.5
25	103.0
26	103.0
27	107.0
28	111.0
29	104.5
30	98.0
31	107.0
32	116.0
33	116.0
34	124.0
35	132.0
36	153.0
37	174.0
38	183.5
39	193.0
40	193.0
41	201.0
42	209.0
43	230.5
44	252.0
45	313.5
46	375.0
47	375.0
48	455.5
49	536.0
50	563.5
51	591.0
52	790.0
53	989.0
54	989.0
55	3218.0
56	5447.0
57	3125.0
58	803.0
59	2052.0
60	3301.0
61	3301.0
62	2356.0
63	1411.0
64	767.5
65	124.0
66	88.5
67	53.0
68	53.0
69	41.0
70	29.0
71	17.5
72	6.0
73	4.0
74	2.0
75	2.0
76	3.0
77	4.0
78	2.5
79	1.0
80	1.0
81	1.0
82	1.0
83	0.5
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	16376.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.39960918417196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.84208479190977	26.954079140205177
2	7.934655775962661	4.982901807523205
3	2.411513029949436	2.27161700048852
4	0.9334889148191364	1.172447484123107
5	0.7779074290159471	1.2212994626282363
6	0.40840140023337224	0.769418661455789
7	0.2528199144301828	0.5556912554958475
8	0.2333722287047841	0.5862237420615535
9	0.11668611435239205	0.32975085490962386
>10	0.8362504861921431	4.524914509037616
>50	0.019447685725398678	0.5923302393746946
>100	0.15558148580318942	11.400830483634588
>500	0.019447685725398678	5.776746458231559
>1k	0.05834305717619603	38.861748900830484
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	2741	16.73790913531998	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	1820	11.11382510991695	TruSeq Adapter, Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	1803	11.010014655593551	No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	946	5.776746458231559	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	492	3.0043966780654614	RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	426	2.6013678553981436	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	202	1.2335124572545189	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	191	1.1663409868099657	No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG	159	0.970933072789448	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	143	0.873229115779189	No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	134	0.8182706399609184	No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT	120	0.7327796775769418	No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT	97	0.5923302393746946	No Hit
CTGATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	45	0.2747923790913532	No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	45	0.2747923790913532	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	43	0.26257938446507084	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	41	0.25036638983878845	No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT	37	0.22594040058622372	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35	0.21372740595994139	No Hit
TCGCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	26	0.15876893014167073	TruSeq Adapter, Index 10 (95% over 21bp)
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	23	0.1404494382022472	No Hit
CTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	22	0.134342940889106	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	18	0.10991695163654128	No Hit
CTTATAACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCG	18	0.10991695163654128	No Hit
TTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	18	0.10991695163654128	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	18	0.10991695163654128	No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT	17	0.1038104543234001	TruSeq Adapter, Index 10 (95% over 23bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.006106497313141182	0.0
15	0.0	0.0	0.0	0.006106497313141182	0.0
16	0.0	0.0	0.0	0.006106497313141182	0.0
17	0.0	0.0	0.0	0.006106497313141182	0.0
18	0.0	0.0	0.0	0.006106497313141182	0.0
19	0.0	0.0	0.0	0.006106497313141182	0.0
20	0.0	0.0	0.0	0.006106497313141182	0.0
21	0.0	0.0	0.0	0.006106497313141182	0.0
22	0.0	0.0	0.0	0.006106497313141182	0.0
23	0.0	0.0	0.0	0.012212994626282364	0.0
24	0.0	0.0	0.0	0.03053248656570591	0.0
25	0.0	0.0	0.0	0.03663898387884709	0.0
26	0.0	0.0	0.0	0.03663898387884709	0.0
27	0.0	0.0	0.0	0.03663898387884709	0.0
28	0.0	0.0	0.0	0.042745481191988274	0.0
29	0.0	0.0	0.0	0.06106497313141182	0.0
30	0.0	0.0	0.0	0.12823644357596484	0.0
31	0.0	0.0	0.0	0.2076209086468002	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	275	0.0	36.327274	1
TTATACA	285	0.0	35.052635	2
TATACAC	295	0.0	33.864407	3
CCGTCTT	295	0.0	27.59322	37
GCCGTCT	305	0.0	26.688524	36
CGTCTTC	70	5.638867E-9	26.42857	37
GGTATCA	60	1.2080545E-6	24.666666	1
TGCCGTC	355	0.0	22.929577	35
ATGCCGT	370	0.0	22.0	34
ATACACA	490	0.0	21.520409	4
TACACAT	505	0.0	20.88119	5
TATGCCG	395	0.0	20.607595	33
AGTACTT	100	1.07338565E-8	20.349998	12
GAGTACT	100	1.07338565E-8	20.349998	11
GTACTTT	115	5.343645E-8	17.695652	13
ACACATC	600	0.0	17.575	6
ACTTTTT	120	8.69386E-8	16.958332	15
TACTTTT	120	8.69386E-8	16.958332	14
TATCAAC	175	5.456968E-12	16.914286	1
CACATCT	625	0.0	16.872	7
>>END_MODULE