FastQCFastQC Report
Fri 10 Feb 2017
ERR1630945.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630945.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008338
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT230502.2859398336668857No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG172541.7111325765765053No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT115361.1440608208755398TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC92010.9124916446667684No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC42050.4170228633652605No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC35280.3498826782289272RNA PCR Primer, Index 7 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27880.27649458812422023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26390.2617177970085428No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG21970.2178832891351908No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA16230.16095793275667486No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15790.15659431658828687No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC14570.14449519903048383No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT13680.13566879359897177No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT13330.1321977352832086No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC13210.13100765814637552No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA12800.1269415612621958No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC12670.12565231103062663No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT12040.11940440606225293No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT11720.11623086703069804No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT10890.10799950016760253No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG10840.10750363469392207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC26550.034.6308861
TTATACA29650.031.322092
TATACAC30550.030.5810153
GGACGGT250.00549602829.59999819
CGTCTTC4800.028.90625237
ATTACGG1450.028.0689662
GGTATCA6050.027.8264481
GATTACG1500.027.1333311
CAACGGT358.8686385E-426.428574
CCGTCTT16300.025.65030737
GCCGTCT16250.025.61538536
TTACGGG1600.025.4375023
GTATCAA18200.023.7857131
TGCCGTC20050.020.94513735
ATGCCGT21000.019.90952334
ATACACA49600.019.0594754
TACACAT50800.018.8641745
GGGTTAG801.6163493E-518.57
ACGGGTT500.007034298518.49999816
TATGCCG22900.018.17685733
Produced by FastQC (version 0.11.5)