##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630945.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1008338
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51053317439192	31.0	31.0	34.0	28.0	34.0
2	31.512301430671066	31.0	31.0	34.0	30.0	34.0
3	30.99027012767544	31.0	31.0	34.0	27.0	34.0
4	35.13551705876402	37.0	35.0	37.0	32.0	37.0
5	35.33197499251243	37.0	35.0	37.0	33.0	37.0
6	35.51669380703692	37.0	35.0	37.0	33.0	37.0
7	35.524851785809915	37.0	35.0	37.0	33.0	37.0
8	35.54226955643842	37.0	35.0	37.0	33.0	37.0
9	37.32084380435925	39.0	37.0	39.0	34.0	39.0
10	37.15574837009019	39.0	37.0	39.0	33.0	39.0
11	37.21918543186908	39.0	37.0	39.0	33.0	39.0
12	37.151658471663275	39.0	37.0	39.0	33.0	39.0
13	37.21543073850237	39.0	37.0	39.0	34.0	39.0
14	38.34903772346178	40.0	38.0	41.0	34.0	41.0
15	38.363169889461666	40.0	38.0	41.0	34.0	41.0
16	38.35242448464701	40.0	38.0	41.0	34.0	41.0
17	38.325376014788695	40.0	38.0	41.0	34.0	41.0
18	38.369940436639304	40.0	38.0	41.0	34.0	41.0
19	38.40305036604789	40.0	38.0	41.0	34.0	41.0
20	38.393899664596596	40.0	38.0	41.0	34.0	41.0
21	38.36057651303432	40.0	38.0	41.0	34.0	41.0
22	38.28289819485133	40.0	38.0	41.0	34.0	41.0
23	38.307246181339984	40.0	38.0	41.0	34.0	41.0
24	38.26313597226327	40.0	38.0	41.0	34.0	41.0
25	38.22716886599533	40.0	38.0	41.0	34.0	41.0
26	38.145861804275945	40.0	38.0	41.0	33.0	41.0
27	38.050983896272875	40.0	37.0	41.0	33.0	41.0
28	38.07128958742009	40.0	37.0	41.0	33.0	41.0
29	38.00810442530183	40.0	37.0	41.0	33.0	41.0
30	37.91541526749959	40.0	37.0	41.0	33.0	41.0
31	37.91604600838211	40.0	37.0	41.0	33.0	41.0
32	37.81027889457702	40.0	37.0	41.0	33.0	41.0
33	37.797438954001535	40.0	37.0	41.0	33.0	41.0
34	37.73417445340749	40.0	37.0	41.0	33.0	41.0
35	37.731510664082876	40.0	37.0	41.0	33.0	41.0
36	37.67764678113886	40.0	37.0	41.0	33.0	41.0
37	37.6100494080358	40.0	37.0	41.0	32.0	41.0
38	37.57273354767945	40.0	37.0	41.0	32.0	41.0
39	37.49453952940383	40.0	37.0	41.0	32.0	41.0
40	37.408399762777954	39.0	37.0	41.0	32.0	41.0
41	37.367174499027115	39.0	37.0	41.0	32.0	41.0
42	37.31481308846835	39.0	36.0	41.0	31.0	41.0
43	36.61957895070899	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	12.0
10	17.0
11	9.0
12	18.0
13	17.0
14	11.0
15	10.0
16	13.0
17	14.0
18	24.0
19	38.0
20	66.0
21	165.0
22	341.0
23	578.0
24	1133.0
25	2013.0
26	3190.0
27	5248.0
28	7855.0
29	11404.0
30	15836.0
31	21448.0
32	28218.0
33	36502.0
34	48571.0
35	65863.0
36	91327.0
37	135545.0
38	239197.0
39	293653.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.7020403872511	22.463201823198172	15.200557749484798	24.634200040065927
2	13.175740674258035	22.20812862353695	41.87355827113527	22.74257243106974
3	14.168661698755775	28.571570247278196	35.70409922069782	21.55566883326821
4	11.558326672207137	19.850089949996928	37.29285219836999	31.29873117942595
5	12.110324117508217	38.57555700568658	36.03563487640057	13.278484000404625
6	26.73795889870262	40.22877249493722	18.00656129194774	15.02670731441243
7	23.413379243864657	34.03243753582628	22.313847142525624	20.240336077783443
8	23.97797167219722	35.83203251290738	22.673250437849212	17.51674537704619
9	23.416949475275157	15.399399804430658	19.40559613938977	41.77805458090442
10	14.200892954545003	29.362475677798518	33.64129885018714	22.79533251746934
11	30.48184239808477	23.078570876035613	25.960045143592726	20.479541582286892
12	19.884998879344028	26.631942860429735	28.976295646896176	24.506762613330054
13	28.40248012075316	20.724003260811354	27.846515751662636	23.02700086677285
14	20.93663037592553	22.943695467194534	26.59108354539847	29.52859061148147
15	23.81175756541953	28.34317461010098	23.270173295065742	24.574894529413747
16	24.67476183581299	27.86853217869405	23.243892424960677	24.212813560532283
17	20.815837546536976	31.090666026669627	24.646596676907944	23.446899749885457
18	23.590998256536995	25.96044183597167	26.283547778621852	24.16501212886949
19	23.667659058767992	27.711937862105763	27.033296374826694	21.58710670429955
20	22.94270373624717	26.740735745355227	26.001598670287144	24.314961848110457
21	24.813405822254044	25.920772598077228	25.571088266037776	23.694733313630945
22	20.560863519970486	31.61628342877091	25.614625254626922	22.208227796631686
23	23.97926092242879	25.852938201277748	26.028474578960626	24.13932629733284
24	23.72964224297805	27.611971382611785	26.4782245635888	22.18016181082137
25	22.147037997179517	28.573057843699235	26.71048795146072	22.569416207660524
26	24.345705507478645	25.737500719004935	26.997693233816438	22.919100539699983
27	20.945952646830726	30.705973592188336	26.273729642242976	22.07434411873796
28	22.769150820459014	25.998425131255594	27.237791296172514	23.99463275211288
29	21.09173709609278	26.482588179757183	27.95253178993552	24.473142934214522
30	22.328028895072883	28.63831374003558	27.912465859662138	21.121191505229397
31	24.36048229859432	25.343882705997395	25.47379946010167	24.82183553530661
32	21.51788388417376	26.24883719546422	28.563140534225628	23.670138386136394
33	23.00290180475198	25.413105526123186	28.27980300256462	23.30418966656022
34	20.652995324980314	28.146613536334048	26.268870160600912	24.931520978084727
35	20.78955667643191	26.17634166321214	32.155685891040505	20.87841576931545
36	22.331698299578118	26.197862224769867	25.24887488124022	26.221564594411795
37	22.52230898766088	27.210320348930615	27.697954455747976	22.569416207660524
38	22.032195553475127	25.275453270629487	28.811172444160587	23.881178731734796
39	23.8718564608296	25.847086988688318	26.67101705975576	23.610039490726322
40	21.06059674434565	25.077999639009935	31.819687446074628	22.04171617056979
41	22.110046432842957	26.430125612641792	26.565992752430233	24.893835202085015
42	23.4868665070641	24.857240330127397	29.35146746428281	22.304425698525694
43	22.698341230817444	24.700348494254904	29.615664588659758	22.985645686267898
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	15.0
2	24.0
3	97.0
4	170.0
5	170.0
6	224.5
7	279.0
8	274.0
9	269.0
10	427.0
11	585.0
12	585.0
13	908.5
14	1232.0
15	3119.0
16	5006.0
17	5655.0
18	6304.0
19	6304.0
20	6922.5
21	7541.0
22	9581.5
23	11622.0
24	14573.0
25	17524.0
26	17524.0
27	21351.5
28	25179.0
29	31819.0
30	38459.0
31	41310.0
32	44161.0
33	44161.0
34	48025.5
35	51890.0
36	54675.5
37	57461.0
38	61810.5
39	66160.0
40	66160.0
41	69683.5
42	73207.0
43	68992.0
44	64777.0
45	66599.5
46	68422.0
47	68422.0
48	68662.0
49	68902.0
50	71019.0
51	73136.0
52	93439.0
53	113742.0
54	113742.0
55	83982.0
56	54222.0
57	58861.5
58	63501.0
59	51036.0
60	38571.0
61	38571.0
62	29681.0
63	20791.0
64	17144.5
65	13498.0
66	11277.0
67	9056.0
68	9056.0
69	7206.0
70	5356.0
71	4471.5
72	3587.0
73	2763.5
74	1940.0
75	1940.0
76	1420.0
77	900.0
78	683.0
79	466.0
80	329.0
81	192.0
82	192.0
83	151.5
84	111.0
85	78.0
86	45.0
87	36.5
88	28.0
89	28.0
90	19.5
91	11.0
92	7.0
93	3.0
94	2.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1008338.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.49758238223652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62153788161177	34.98468358905149
2	12.564367992625655	11.181679996651916
3	3.670696563688606	4.900113682287788
4	1.4997247796395101	2.6693650773076243
5	0.8060304038373058	1.7933202148668947
6	0.49866988239009163	1.331376250391598
7	0.34294408034653684	1.0682127727404254
8	0.24155782543932944	0.8598991390048377
9	0.19200184473654644	0.7689256113335274
>10	1.2440588014966403	10.897875738649535
>50	0.16143295507020397	5.01625770808547
>100	0.142726595786326	12.4557646383262
>500	0.009351820623791966	2.8404077520561533
>1k	0.004007923124482271	3.1842132110918326
>5k	2.2266239580457061E-4	0.9116294030510906
>10k+	6.679871874137118E-4	5.136275215103635
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT	23050	2.2859398336668857	No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG	17254	1.7111325765765053	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	11536	1.1440608208755398	TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC	9201	0.9124916446667684	No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC	4205	0.4170228633652605	No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC	3528	0.3498826782289272	RNA PCR Primer, Index 7 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2788	0.27649458812422023	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2639	0.2617177970085428	No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG	2197	0.2178832891351908	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA	1623	0.16095793275667486	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1579	0.15659431658828687	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1457	0.14449519903048383	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1368	0.13566879359897177	No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT	1333	0.1321977352832086	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1321	0.13100765814637552	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1280	0.1269415612621958	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1267	0.12565231103062663	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1204	0.11940440606225293	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1172	0.11623086703069804	No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT	1089	0.10799950016760253	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1084	0.10750363469392207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.917309473609048E-5	0.0
10	0.0	0.0	0.0	1.9834618947218096E-4	0.0
11	0.0	1.9834618947218096E-4	0.0	2.9751928420827144E-4	0.0
12	0.0	1.9834618947218096E-4	0.0	2.9751928420827144E-4	0.0
13	0.0	1.9834618947218096E-4	0.0	2.9751928420827144E-4	0.0
14	0.0	1.9834618947218096E-4	0.0	2.9751928420827144E-4	0.0
15	0.0	1.9834618947218096E-4	0.0	6.942116631526333E-4	0.0
16	0.0	1.9834618947218096E-4	0.0	7.933847578887238E-4	0.0
17	0.0	1.9834618947218096E-4	0.0	8.925578526248143E-4	0.0
18	0.0	1.9834618947218096E-4	0.0	8.925578526248143E-4	0.0
19	0.0	1.9834618947218096E-4	0.0	9.917309473609047E-4	0.0
20	0.0	1.9834618947218096E-4	0.0	0.0011900771368330858	0.0
21	0.0	1.9834618947218096E-4	0.0	0.0014875964210413573	0.0
22	0.0	1.9834618947218096E-4	0.0	0.0014875964210413573	0.0
23	0.0	1.9834618947218096E-4	0.0	0.001884288799985719	0.0
24	0.0	1.9834618947218096E-4	0.0	0.0020826349894579	0.0
25	0.0	1.9834618947218096E-4	0.0	0.0020826349894579	0.0
26	0.0	1.9834618947218096E-4	0.0	0.002280981178930081	0.0
27	0.0	1.9834618947218096E-4	0.0	0.0029751928420827145	0.0
28	0.0	1.9834618947218096E-4	0.0	0.008528886147303781	0.0
29	0.0	1.9834618947218096E-4	0.0	0.019239580378801552	0.0
30	0.0	1.9834618947218096E-4	0.0	0.03312381364185422	0.0
31	0.0	1.9834618947218096E-4	0.0	0.07904095650466411	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2655	0.0	34.630886	1
TTATACA	2965	0.0	31.32209	2
TATACAC	3055	0.0	30.581015	3
GGACGGT	25	0.005496028	29.599998	19
CGTCTTC	480	0.0	28.906252	37
ATTACGG	145	0.0	28.068966	2
GGTATCA	605	0.0	27.826448	1
GATTACG	150	0.0	27.133331	1
CAACGGT	35	8.8686385E-4	26.42857	4
CCGTCTT	1630	0.0	25.650307	37
GCCGTCT	1625	0.0	25.615385	36
TTACGGG	160	0.0	25.437502	3
GTATCAA	1820	0.0	23.785713	1
TGCCGTC	2005	0.0	20.945137	35
ATGCCGT	2100	0.0	19.909523	34
ATACACA	4960	0.0	19.059475	4
TACACAT	5080	0.0	18.864174	5
GGGTTAG	80	1.6163493E-5	18.5	7
ACGGGTT	50	0.0070342985	18.499998	16
TATGCCG	2290	0.0	18.176857	33
>>END_MODULE