FastQCFastQC Report
Fri 10 Feb 2017
ERR1630944.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630944.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198732
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT196479.886178370871324No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT154607.7793208944709455RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG148807.487470563371777No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC73463.6964354004387823No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC64013.2209206368375503RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC43062.166737113298311RNA PCR Primer, Index 39 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22751.1447577642251878No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20541.0335527242718836No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA16400.8252319706942013No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG15510.7804480405772598No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT13270.6677334299458567No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11130.5600507215747842No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT9330.4694764808888352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8440.42469255077189383No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT6900.34720125596280416Illumina PCR Primer Index 11 (95% over 24bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6640.3341183100859449No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5630.28329609725660687No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4930.2480727814342934No Hit
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT4880.24555683030412817Illumina PCR Primer Index 11 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4880.24555683030412817No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4680.2354930257834672No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4510.22693879194090535No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4270.21486222651611214No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4190.21083670470784777No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4010.20177928063925288No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3140.15800173097437756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.15749854074834452No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2790.14039007306322082No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2790.14039007306322082No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG2780.13988688283718778No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2750.13837731215908863No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2740.13787412193305557No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2740.13787412193305557No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG2700.13586136102892338No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2660.1338486001247912No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2630.132339029446692No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2590.13032626854255983No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG2540.12781031741239457No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2480.12479117605619627No Hit
TCGCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT2460.1237847956041302RNA PCR Primer, Index 39 (95% over 23bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2460.1237847956041302No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2360.11875289334379968No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2330.11724332266570055No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2310.11623694221363444No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2310.11623694221363444No Hit
CTGATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT2300.1157337519876014No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA2280.11472737153553529No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2260.11372099108346918No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA2210.11120503995330396No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2210.11120503995330396No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2130.10717951814503955No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2030.10214761588470905No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2010.10114123543264295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCCT200.00183827537.036
TTATCAA200.00183827537.02
ATTACGG452.5975169E-937.02
TCGCCGA501.7826096E-1037.01
GATTACG452.5975169E-937.01
GCCGAGC501.7826096E-1037.03
TTACGGG452.5975169E-937.03
CGCCGAG501.7826096E-1037.02
CTTATAC20550.036.189781
CAGACAC507.2668627E-933.30000318
TTATACA22600.032.907082
TTTACAC352.3784505E-531.7142872
CGTCTTC8450.031.08875737
TATACAC24650.030.3204883
CCAATAG250.00548554329.623
GTTTCCT250.00548554329.633
GGGAGGT250.00548554329.613
ATGGACG250.00548554329.67
AATGGAC250.00548554329.66
TGGACGG250.00548554329.68
Produced by FastQC (version 0.11.5)