##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630944.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	198732
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16458849103315	31.0	31.0	34.0	28.0	34.0
2	31.259283859670308	31.0	31.0	34.0	28.0	34.0
3	30.72852384115291	31.0	31.0	34.0	26.0	34.0
4	34.834852967815955	37.0	35.0	37.0	32.0	37.0
5	35.02313165469074	35.0	35.0	37.0	32.0	37.0
6	35.20090372964596	37.0	35.0	37.0	32.0	37.0
7	35.164618682446715	37.0	35.0	37.0	32.0	37.0
8	35.21759454944347	37.0	35.0	37.0	32.0	37.0
9	37.05853108709216	39.0	37.0	39.0	33.0	39.0
10	36.85746130467162	39.0	37.0	39.0	32.0	39.0
11	36.889308214077246	39.0	37.0	39.0	33.0	39.0
12	36.79042630275949	39.0	37.0	39.0	32.0	39.0
13	36.92888412535475	39.0	37.0	39.0	33.0	39.0
14	37.67773181973713	39.0	37.0	41.0	32.0	41.0
15	37.654041623895495	39.0	37.0	41.0	32.0	41.0
16	37.66304872894149	39.0	37.0	41.0	32.0	41.0
17	37.56784010627378	39.0	37.0	41.0	32.0	41.0
18	37.8023871344323	39.0	37.0	41.0	32.0	41.0
19	37.75665720669042	39.0	37.0	41.0	32.0	41.0
20	37.847286798301226	39.0	37.0	41.0	33.0	41.0
21	37.778037759394564	39.0	37.0	41.0	32.0	41.0
22	37.5413773322867	39.0	36.0	41.0	32.0	41.0
23	37.73188515186281	39.0	37.0	41.0	33.0	41.0
24	37.51459754845722	39.0	36.0	41.0	32.0	41.0
25	37.310035625868004	39.0	36.0	40.0	31.0	41.0
26	37.268779059235555	39.0	36.0	40.0	31.0	41.0
27	37.064181913330515	39.0	36.0	40.0	31.0	41.0
28	37.22152446510879	39.0	36.0	40.0	31.0	41.0
29	37.126074311132584	39.0	36.0	40.0	31.0	41.0
30	37.03058893384055	39.0	35.0	40.0	31.0	41.0
31	36.976455729323916	39.0	35.0	40.0	31.0	41.0
32	36.60922247046273	38.0	35.0	40.0	30.0	41.0
33	36.446903367348995	38.0	35.0	40.0	30.0	41.0
34	36.35658575367832	38.0	35.0	40.0	30.0	41.0
35	36.31641104603184	38.0	35.0	40.0	30.0	41.0
36	36.21853048326389	38.0	35.0	40.0	30.0	41.0
37	36.148868828371874	38.0	35.0	40.0	30.0	41.0
38	36.082286697663186	38.0	35.0	40.0	30.0	41.0
39	35.75366825674778	38.0	35.0	40.0	29.0	41.0
40	35.33421894813115	38.0	34.0	40.0	26.0	41.0
41	35.36735905641769	38.0	34.0	40.0	26.0	41.0
42	35.195841635972066	38.0	34.0	40.0	26.0	41.0
43	34.13467886399775	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	22.0
11	22.0
12	15.0
13	18.0
14	10.0
15	10.0
16	12.0
17	20.0
18	12.0
19	36.0
20	50.0
21	79.0
22	153.0
23	274.0
24	475.0
25	848.0
26	1335.0
27	1921.0
28	2821.0
29	3942.0
30	5030.0
31	6366.0
32	7829.0
33	9857.0
34	12481.0
35	16218.0
36	21543.0
37	29572.0
38	42305.0
39	35447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.513938369261115	23.28512770967937	19.28929412475092	29.9116397963086
2	9.902280458104382	18.811263410019524	45.84817744500131	25.43827868687479
3	11.787734235050218	30.294567558319745	40.490711108427426	17.426987098202602
4	7.836181390012681	22.89565847472979	24.11035968037357	45.15780045488396
5	12.765936034458466	35.632912666304364	31.575186683573857	20.025964615663305
6	25.422679789867765	37.704546826882435	14.128575166555965	22.74419821669384
7	19.564035988164967	35.76172936416883	13.191634965682427	31.482599681983775
8	27.587907332487976	29.39385705372059	24.11035968037357	18.90787593341787
9	17.132620815973272	10.859348268019243	12.518366443250207	59.48966447275728
10	13.976108528067952	27.835476923696234	25.753275768371473	32.43513877986434
11	25.607853793047923	18.05194935893565	24.545619225892157	31.79457762212427
12	15.856530402753458	27.53758830988467	17.995592053619948	38.61028923374192
13	35.14230219592215	13.495058671980356	25.185174003180162	26.177465128917337
14	23.690699031862007	26.29168931022684	18.278384960650524	31.73922669726063
15	28.808646820844153	25.248072781434296	15.166656602862147	30.776623794859407
16	34.655717247348186	20.48034538977115	14.70875349717207	30.15518386570859
17	16.028118269830728	39.65239619185637	15.235090473602641	29.08439506471026
18	28.55956765895779	13.918744842300182	17.58146649759475	39.94022100114727
19	20.935229354105026	24.235654046655796	21.311112452951715	33.51800414628746
20	16.68578789525592	17.540708089286074	26.299740353843365	39.47376366161463
21	27.04194593724211	15.433347422659663	21.302055028883117	36.222651611215106
22	14.905500875550992	36.90447436748989	28.425719058833	19.76430569812612
23	23.157317392266975	14.974941126743554	22.303403578688886	39.564337902300586
24	25.198256949057026	25.90372964595536	29.435118652255298	19.462894752732325
25	15.089165308053056	34.93700058370066	22.54795402854095	27.425880079705333
26	24.842501459251658	19.30539621198398	20.868808244268664	34.983294084495704
27	14.269971620071251	35.36924098786305	34.243604452227125	16.117182939838575
28	12.60793430348409	15.97729605700139	28.198277076666063	43.21649256284846
29	13.952961777670431	17.558822937423262	33.46265322142383	35.02556206348248
30	13.490529959946057	19.073425517782745	40.26125636535636	27.174788156914843
31	19.30539621198398	14.138135780850591	27.137552080188392	39.418915926977036
32	20.580480244751726	26.685184066984686	36.03395527645271	16.70038041181088
33	13.783889861723326	18.60092989553771	32.16190648712839	35.45327375561057
34	14.952800756798098	31.810176519131296	33.08626693235111	20.1507557917195
35	17.147716522754262	27.299579332971035	39.924118913914214	15.628585230360487
36	23.06221443954673	18.22504679669102	19.126260491516213	39.58647827224604
37	18.958698146247208	26.163375802588412	28.57214741460862	26.305778636555765
38	19.557494515226537	15.579775778435279	32.29877422860938	32.563955477728804
39	30.59748807439164	17.684620493931526	23.390797657146308	28.327093774530525
40	15.462532455769578	15.567699213010485	47.565062496226076	21.404705834993862
41	21.94211299639716	21.355896383068657	22.13533804319385	34.56665257734034
42	24.621600950023147	15.646700078497675	39.73542257915182	19.996276392327356
43	23.083348429040115	15.648209649175774	37.02725278264195	24.24118913914216
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	4.0
2	8.0
3	45.5
4	83.0
5	83.0
6	146.0
7	209.0
8	226.5
9	244.0
10	349.5
11	455.0
12	455.0
13	705.5
14	956.0
15	2208.5
16	3461.0
17	3474.5
18	3488.0
19	3488.0
20	2883.5
21	2279.0
22	1744.5
23	1210.0
24	1237.5
25	1265.0
26	1265.0
27	1340.0
28	1415.0
29	1355.0
30	1295.0
31	1386.0
32	1477.0
33	1477.0
34	1628.5
35	1780.0
36	1890.5
37	2001.0
38	2186.0
39	2371.0
40	2371.0
41	2934.0
42	3497.0
43	4199.5
44	4902.0
45	6695.5
46	8489.0
47	8489.0
48	12689.0
49	16889.0
50	32834.0
51	48779.0
52	32404.5
53	16030.0
54	16030.0
55	27181.0
56	38332.0
57	30741.5
58	23151.0
59	14649.0
60	6147.0
61	6147.0
62	4953.0
63	3759.0
64	3017.5
65	2276.0
66	1817.0
67	1358.0
68	1358.0
69	1026.5
70	695.0
71	485.5
72	276.0
73	175.5
74	75.0
75	75.0
76	60.0
77	45.0
78	33.5
79	22.0
80	14.0
81	6.0
82	6.0
83	4.0
84	2.0
85	1.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	198732.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.155948714852165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.3633402505976	13.86188434675845
2	10.990569754918699	4.210695811444558
3	4.549633560114529	2.6145764144677255
4	2.5900338858388716	1.9845822514743472
5	1.6128608579158896	1.544793993921462
6	1.0664845412277706	1.2257713906165086
7	0.8852347054033465	1.187025743211964
8	0.6619559221413749	1.014431495682628
9	0.5726444088365862	0.9872592234768431
>10	3.9559746775591687	14.944246522955536
>50	0.48070608631695083	6.502224100799066
>100	0.2259055924768184	8.814886379646962
>500	0.01313404607423363	1.858784694966085
>1k	0.01838766450392708	7.178511764587485
>5k	0.005253618429693451	6.917356037276331
>10k+	0.007880427644540177	25.152969828714046
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	19647	9.886178370871324	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	15460	7.7793208944709455	RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	14880	7.487470563371777	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	7346	3.6964354004387823	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	6401	3.2209206368375503	RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	4306	2.166737113298311	RNA PCR Primer, Index 39 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2275	1.1447577642251878	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2054	1.0335527242718836	No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	1640	0.8252319706942013	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	1551	0.7804480405772598	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT	1327	0.6677334299458567	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1113	0.5600507215747842	No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	933	0.4694764808888352	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	844	0.42469255077189383	No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT	690	0.34720125596280416	Illumina PCR Primer Index 11 (95% over 24bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	664	0.3341183100859449	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	563	0.28329609725660687	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	493	0.2480727814342934	No Hit
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT	488	0.24555683030412817	Illumina PCR Primer Index 11 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	488	0.24555683030412817	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	468	0.2354930257834672	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	451	0.22693879194090535	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	427	0.21486222651611214	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	419	0.21083670470784777	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	401	0.20177928063925288	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	314	0.15800173097437756	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	313	0.15749854074834452	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	279	0.14039007306322082	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	279	0.14039007306322082	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	278	0.13988688283718778	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	275	0.13837731215908863	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	274	0.13787412193305557	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	274	0.13787412193305557	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	270	0.13586136102892338	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	266	0.1338486001247912	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	263	0.132339029446692	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	259	0.13032626854255983	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	254	0.12781031741239457	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	248	0.12479117605619627	No Hit
TCGCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	246	0.1237847956041302	RNA PCR Primer, Index 39 (95% over 23bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	246	0.1237847956041302	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	236	0.11875289334379968	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	233	0.11724332266570055	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	231	0.11623694221363444	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	231	0.11623694221363444	No Hit
CTGATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	230	0.1157337519876014	No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA	228	0.11472737153553529	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	226	0.11372099108346918	No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	221	0.11120503995330396	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	221	0.11120503995330396	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	213	0.10717951814503955	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	203	0.10214761588470905	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	201	0.10114123543264295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.031902260330495E-4	0.0
6	0.0	0.0	0.0	5.031902260330495E-4	0.0
7	0.0	0.0	0.0	5.031902260330495E-4	0.0
8	0.0	0.0	0.0	5.031902260330495E-4	0.0
9	0.0	0.0	0.0	5.031902260330495E-4	0.0
10	0.0	0.0	0.0	0.001006380452066099	0.0
11	0.0	0.0	0.0	0.0030191413561982973	0.0
12	0.0	0.0	0.0	0.0030191413561982973	0.0
13	0.0	0.0	0.0	0.0030191413561982973	0.0
14	0.0	0.0	0.0	0.0030191413561982973	0.0
15	0.0	0.0	0.0	0.0030191413561982973	0.0
16	0.0	0.0	0.0	0.0030191413561982973	0.0
17	0.0	0.0	0.0	0.0030191413561982973	0.0
18	0.0	0.0	0.0	0.0030191413561982973	0.0
19	5.031902260330495E-4	0.0	0.0	0.0030191413561982973	0.0
20	5.031902260330495E-4	0.0	0.0	0.0030191413561982973	0.0
21	5.031902260330495E-4	0.0	0.0	0.0035223315822313465	0.0
22	5.031902260330495E-4	0.0	0.0	0.005535092486363545	0.0
23	5.031902260330495E-4	0.0	0.0	0.007547853390495743	0.0
24	5.031902260330495E-4	0.0	0.0	0.008051043616528792	0.0
25	5.031902260330495E-4	0.0	0.0	0.009057424068594892	0.0
26	5.031902260330495E-4	0.0	0.0	0.00956061429462794	0.0
27	5.031902260330495E-4	0.0	0.0	0.012579755650826238	0.0
28	5.031902260330495E-4	0.0	0.0	0.026669081979751624	0.0
29	5.031902260330495E-4	0.0	0.0	0.060886017349998996	0.0
30	5.031902260330495E-4	0.0	0.0	0.101644425658676	0.0
31	5.031902260330495E-4	0.0	0.0	0.1615240625566089	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTCCT	20	0.001838275	37.0	36
TTATCAA	20	0.001838275	37.0	2
ATTACGG	45	2.5975169E-9	37.0	2
TCGCCGA	50	1.7826096E-10	37.0	1
GATTACG	45	2.5975169E-9	37.0	1
GCCGAGC	50	1.7826096E-10	37.0	3
TTACGGG	45	2.5975169E-9	37.0	3
CGCCGAG	50	1.7826096E-10	37.0	2
CTTATAC	2055	0.0	36.18978	1
CAGACAC	50	7.2668627E-9	33.300003	18
TTATACA	2260	0.0	32.90708	2
TTTACAC	35	2.3784505E-5	31.714287	2
CGTCTTC	845	0.0	31.088757	37
TATACAC	2465	0.0	30.320488	3
CCAATAG	25	0.005485543	29.6	23
GTTTCCT	25	0.005485543	29.6	33
GGGAGGT	25	0.005485543	29.6	13
ATGGACG	25	0.005485543	29.6	7
AATGGAC	25	0.005485543	29.6	6
TGGACGG	25	0.005485543	29.6	8
>>END_MODULE