FastQCFastQC Report
Fri 10 Feb 2017
ERR1630943.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630943.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387332
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT175754.537451075563083No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG164474.246228042093088No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT122093.1520762549957144RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC91242.3556019125711276No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC44031.136750901035804No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC42871.106802433054847RNA PCR Primer, Index 47 (95% over 23bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG21600.5576611279212664No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA15220.3929445540260036No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT13350.3446655582291161No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11390.2940629743992234No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT11050.28528497516342566No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10930.2821868577860853No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA10480.27056891762105895No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC7120.1838216310555286No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA6440.16626563258393318No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT6020.15542222176324186No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC5750.14845145766422604No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA5680.14664422252744416No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC5550.14328792870199208No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5400.1394152819803166No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT5080.1311536356407423No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG4870.12573193023039667No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA4670.1205684012681627No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT4520.11669575454648724No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA4440.11463034296159368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4170.10765957886257785No Hit
ATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCT4150.10714322596635445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC19700.035.7791861
CGTCTTC5000.034.0437
TTATACA20950.033.644392
TATACAC22500.031.6555563
AGCTTAT303.595111E-430.83333410
TTACGGG303.595111E-430.8333343
GATTACG303.595111E-430.8333341
AGTCTAA250.00549190129.59999810
CCGTCTT17600.026.48863637
GCCGTCT17900.025.94134136
GTATCAA6550.024.2900771
GGTATCA2250.023.8444441
TCGCCAT903.808964E-922.6111113
TATAGAA502.6976224E-422.1999992
ATTACGG450.003821297820.5555552
TGCCGTC22800.020.44736935
GTGTGGG555.1346375E-420.1818181
AGTACAA555.1346375E-420.18181810
GTCGCCA1052.2479071E-819.3809512
ACGTGGG1254.0927262E-1019.2436
Produced by FastQC (version 0.11.5)