FastQCFastQC Report
Fri 10 Feb 2017
ERR1630942.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630942.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427905
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT163583.822811137986235No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG136333.185987543964198No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT116772.728876736658838TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC70311.6431217209427327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42320.9890045687711057No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC41210.9630642315467218RNA PCR Primer, Index 1 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40330.9424989191526156No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC37630.8794008015797898No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA22470.5251165562449609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15900.37157780348441827No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG15410.3601266636286092No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA14870.347507040114044No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12630.29515897220177373No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11960.27950129117444295No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT11560.2701534219043947No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10340.24164242063074748No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9880.23089237097019197No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9670.22598473960341667No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9540.22294668209065097No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9530.22271298535889977No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT8990.2100933618443346No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8480.19817482852502308No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8040.18789217232797No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6500.1519028756382842No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5980.13975064558722147No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5560.1299353828536708No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5510.12876689919491477No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5430.1268973253409051No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5420.1266636286091539No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.1266636286091539No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5380.12572884168214907No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5220.12198969397412976No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4880.11404400509458874No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4740.11077225085007185No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4690.10960376719131584No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4620.1079678900690574No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT4360.10189177504352602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC4100.035.1951237
CTTATAC20450.034.5574571
ATTACGG700.034.3571432
GATTACG750.032.0666661
TTATACA22650.031.282562
TATACAC24100.029.937763
GCCTAGT502.7140413E-729.5999981
CCGTCTT17000.027.4235337
GCCGTCT17350.026.97694636
CGTTACT358.860261E-426.4285721
GGTATCA6300.026.1349221
GCGGTAT551.899043E-523.5454546
TGCGGTA551.899043E-523.5454545
TATGCGG551.899043E-523.5454543
CGCCGTT551.899043E-523.54545425
TTACCGG551.899043E-523.54545431
TACCGGG551.899043E-523.54545432
ACTGTTC400.001929216923.1250028
GTATCAA15800.022.1297451
TTACGGG1107.4578566E-1121.8636363
Produced by FastQC (version 0.11.5)