##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630942.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427905
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44558722146271	31.0	31.0	34.0	28.0	34.0
2	31.445110480129934	31.0	31.0	34.0	28.0	34.0
3	30.915831785092486	31.0	31.0	34.0	27.0	34.0
4	35.05879108680665	37.0	35.0	37.0	32.0	37.0
5	35.266886341594514	37.0	35.0	37.0	33.0	37.0
6	35.47038478166883	37.0	35.0	37.0	33.0	37.0
7	35.46684661315012	37.0	35.0	37.0	33.0	37.0
8	35.495813323050676	37.0	35.0	37.0	33.0	37.0
9	37.281623257498744	39.0	37.0	39.0	34.0	39.0
10	37.12312546009044	39.0	37.0	39.0	33.0	39.0
11	37.19652492959886	39.0	37.0	39.0	33.0	39.0
12	37.09739778689195	39.0	37.0	39.0	33.0	39.0
13	37.19545693553476	39.0	37.0	39.0	34.0	39.0
14	38.20881737768897	40.0	38.0	41.0	33.0	41.0
15	38.21464577417885	40.0	38.0	41.0	33.0	41.0
16	38.22443533027191	40.0	38.0	41.0	33.0	41.0
17	38.19429312581064	40.0	38.0	41.0	33.0	41.0
18	38.31516107547236	40.0	38.0	41.0	34.0	41.0
19	38.28363538635912	40.0	38.0	41.0	34.0	41.0
20	38.27041983617859	40.0	38.0	41.0	34.0	41.0
21	38.251366541638916	40.0	38.0	41.0	34.0	41.0
22	38.086846379453384	40.0	37.0	41.0	33.0	41.0
23	38.15048199950924	40.0	37.0	41.0	33.0	41.0
24	38.02942008156016	40.0	37.0	41.0	33.0	41.0
25	37.99234643203515	40.0	37.0	41.0	33.0	41.0
26	37.90013671258807	40.0	37.0	41.0	33.0	41.0
27	37.72822705974457	40.0	37.0	41.0	33.0	41.0
28	37.78702282048585	40.0	37.0	41.0	33.0	41.0
29	37.67906194131875	40.0	37.0	41.0	33.0	41.0
30	37.51534803285776	39.0	36.0	41.0	32.0	41.0
31	37.47059277176009	39.0	36.0	41.0	32.0	41.0
32	37.28789567777895	39.0	36.0	41.0	32.0	41.0
33	37.24328530865496	39.0	36.0	41.0	32.0	41.0
34	37.11177948376392	39.0	36.0	41.0	31.0	41.0
35	37.091826456807	39.0	36.0	41.0	31.0	41.0
36	37.029408396723575	39.0	35.0	41.0	31.0	41.0
37	36.91569857795539	39.0	35.0	41.0	31.0	41.0
38	36.84179198653907	39.0	35.0	41.0	31.0	41.0
39	36.70428950351129	39.0	35.0	41.0	30.0	41.0
40	36.49084259356633	39.0	35.0	40.0	30.0	41.0
41	36.431413514682	39.0	35.0	40.0	30.0	41.0
42	36.282574403196975	39.0	35.0	40.0	30.0	41.0
43	35.49543473434524	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	11.0
11	16.0
12	13.0
13	10.0
14	10.0
15	9.0
16	11.0
17	16.0
18	15.0
19	47.0
20	51.0
21	112.0
22	206.0
23	416.0
24	746.0
25	1200.0
26	1900.0
27	2952.0
28	4339.0
29	5949.0
30	8166.0
31	10629.0
32	13352.0
33	17418.0
34	22778.0
35	30642.0
36	41867.0
37	61732.0
38	98579.0
39	104698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.12227013005223	23.061193489209053	16.316939507600985	24.49959687313773
2	12.531286149963192	22.257977822180155	42.98080181348664	22.22993421437001
3	14.008015797899068	30.33033033033033	35.73199658802771	19.929657283742888
4	9.90593706547014	20.40733340344235	35.29498369965296	34.39174583143455
5	12.225143431369112	38.13299681003961	34.0930814082565	15.548778350334771
6	25.97469064395134	39.8156132786483	17.122725838679145	17.08697023872121
7	22.588658697608114	33.79745504259123	20.474404365454948	23.139481894345707
8	25.38086724857153	32.89842371554434	23.90109954312289	17.81960949276124
9	22.143933817085568	13.346887743774904	18.72354845117491	45.78562998796462
10	14.316962877274161	28.70824131524521	31.677591988876035	25.297203818604597
11	30.00128533202463	22.067748682534674	24.860658323693343	23.07030766174735
12	19.38210584124981	27.22403337189329	27.055538028300674	26.33832275855622
13	31.239644313574278	19.419964711793504	26.48905715053575	22.85133382409647
14	21.399843423189726	24.032203409635315	26.472932076044913	28.09502109113004
15	25.440459915168084	27.413795118075274	22.333695563267547	24.81204940348909
16	25.667379441698507	25.596569331977893	22.135287038010773	26.600764188312827
17	19.12994706769026	32.14919199354997	23.706430165574137	25.014430773185637
18	23.99434453909162	22.093455323027307	26.85736319977565	27.05483693810542
19	22.821654339164066	27.155793926221943	28.515908905013966	21.50664282960003
20	21.812084457998854	26.00343534195674	25.820217104263797	26.364263095780604
21	28.946378284899687	22.6246479942978	26.42736121335343	22.001612507449085
22	21.84760636122504	32.724553347121436	25.869994508126805	19.557845783526716
23	23.621364555216694	23.44071698157301	26.40702959769108	26.530888865519213
24	23.899697362732383	28.825323377852563	28.457718418807914	18.817260840607144
25	20.698285834472603	29.21863497738984	28.320538437269953	21.762540750867597
26	28.110445075425623	24.775592713335904	28.710344585830967	18.40361762540751
27	20.913987917878966	32.67033570535516	28.014629415407626	18.401046961358244
28	21.81114967107185	23.748729274021102	29.467054603241372	24.973066451665673
29	23.06469894018532	25.763662495180007	30.080041130624792	21.091597434009884
30	19.55036748811068	27.04805973288463	31.280073848167234	22.12149893083745
31	27.810612168588822	25.439992521704585	24.775592713335904	21.97380259637069
32	24.17008448136853	24.231780418550848	31.458618151225153	20.13951694885547
33	21.64452390133324	23.753169511924376	31.121160070576416	23.48114651616597
34	18.59174349446723	32.46234561409659	27.64679075963123	21.29912013180496
35	19.50059008424767	24.000654350848905	38.142578376041406	18.356177188862013
36	26.17169698881761	24.06164919783597	25.899673993059206	23.866979820287213
37	21.114733410453255	26.99290730419135	29.455136069922062	22.437223215433335
38	20.356854909384094	22.940839672357182	30.623619728678094	26.078685689580634
39	24.963952279127376	23.58911440623503	27.490915039553173	23.956018275084425
40	18.999544291373084	23.458244236454355	36.66584872810554	20.876362744067023
41	22.513174653252474	24.64472254355523	26.369404423879132	26.472698379313165
42	22.670218856989287	22.954160386067002	33.14754443159112	21.22807632535259
43	22.70036573538519	22.695224407286666	31.924843131068815	22.679566726259335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	7.0
2	13.0
3	97.5
4	182.0
5	182.0
6	251.5
7	321.0
8	345.5
9	370.0
10	564.5
11	759.0
12	759.0
13	1233.5
14	1708.0
15	4134.0
16	6560.0
17	6855.5
18	7151.0
19	7151.0
20	6425.0
21	5699.0
22	5532.0
23	5365.0
24	6348.0
25	7331.0
26	7331.0
27	8751.5
28	10172.0
29	11486.0
30	12800.0
31	14042.5
32	15285.0
33	15285.0
34	16619.5
35	17954.0
36	18445.5
37	18937.0
38	19514.5
39	20092.0
40	20092.0
41	20073.0
42	20054.0
43	20701.0
44	21348.0
45	23353.5
46	25359.0
47	25359.0
48	29760.5
49	34162.0
50	46676.5
51	59191.0
52	44814.5
53	30438.0
54	30438.0
55	38400.0
56	46362.0
57	38107.5
58	29853.0
59	21137.0
60	12421.0
61	12421.0
62	10253.0
63	8085.0
64	6386.5
65	4688.0
66	3738.5
67	2789.0
68	2789.0
69	2182.5
70	1576.0
71	1094.5
72	613.0
73	376.0
74	139.0
75	139.0
76	101.0
77	63.0
78	47.0
79	31.0
80	26.5
81	22.0
82	22.0
83	12.5
84	3.0
85	3.5
86	4.0
87	3.5
88	3.0
89	3.0
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	427905.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.3801964586242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.89077318089977	34.65677435818817
2	12.188235226325876	10.57456077203881
3	3.4566138217374496	4.498457600056992
4	1.3135816681442976	2.279337233141877
5	0.6565572270996186	1.42407907489555
6	0.40856249622798646	1.0634112811197578
7	0.26813603855527096	0.8142255821115422
8	0.21628654704894812	0.7506042321872668
9	0.1432446101978417	0.5592581398819275
>10	1.1961245528235194	10.402676212481008
>50	0.14387488983708988	4.3411075671188
>100	0.10076630861249362	8.107639591726262
>500	0.00862171624491924	2.677690333387854
>1k	0.00646628718368943	6.466429305325902
>5k	5.388572653074524E-4	1.643547859803579
>10k+	0.0016165717959223576	9.74020085653471
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	16358	3.822811137986235	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	13633	3.185987543964198	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	11677	2.728876736658838	TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	7031	1.6431217209427327	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4232	0.9890045687711057	No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC	4121	0.9630642315467218	RNA PCR Primer, Index 1 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4033	0.9424989191526156	No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	3763	0.8794008015797898	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2247	0.5251165562449609	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1590	0.37157780348441827	No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG	1541	0.3601266636286092	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	1487	0.347507040114044	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1263	0.29515897220177373	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1196	0.27950129117444295	No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT	1156	0.2701534219043947	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1034	0.24164242063074748	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	988	0.23089237097019197	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	967	0.22598473960341667	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	954	0.22294668209065097	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	953	0.22271298535889977	No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	899	0.2100933618443346	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	848	0.19817482852502308	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	804	0.18789217232797	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	650	0.1519028756382842	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	598	0.13975064558722147	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	556	0.1299353828536708	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	551	0.12876689919491477	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	543	0.1268973253409051	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	542	0.1266636286091539	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	542	0.1266636286091539	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	538	0.12572884168214907	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	522	0.12198969397412976	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	488	0.11404400509458874	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	474	0.11077225085007185	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	469	0.10960376719131584	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	462	0.1079678900690574	No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT	436	0.10189177504352602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.3369673175120646E-4	0.0
8	0.0	0.0	0.0	2.3369673175120646E-4	0.0
9	0.0	0.0	0.0	7.010901952536194E-4	0.0
10	0.0	0.0	0.0	0.0011684836587560323	0.0
11	0.0	0.0	0.0	0.0014021803905072388	0.0
12	0.0	0.0	0.0	0.0014021803905072388	0.0
13	0.0	0.0	0.0	0.0018695738540096516	0.0
14	0.0	0.0	0.0	0.0018695738540096516	0.0
15	0.0	0.0	0.0	0.002103270585760858	0.0
16	0.0	0.0	0.0	0.002103270585760858	0.0
17	0.0	0.0	0.0	0.002103270585760858	0.0
18	0.0	0.0	0.0	0.002103270585760858	0.0
19	0.0	0.0	0.0	0.002103270585760858	0.0
20	0.0	0.0	0.0	0.0025706640492632713	0.0
21	0.0	0.0	0.0	0.0032717542445168902	0.0
22	0.0	0.0	0.0	0.00397284443977051	0.0
23	0.0	0.0	0.0	0.004907631366775336	0.0
24	0.0	0.0	0.0	0.0065435084890337804	0.0
25	0.0	0.0	0.0	0.006777205220784987	0.0
26	0.0	0.0	0.0	0.007010901952536194	0.0
27	0.0	0.0	0.0	0.009581566001799465	0.0
28	0.0	0.0	0.0	0.01846204180834531	0.0
29	0.0	0.0	0.0	0.04557086269148526	0.0
30	0.0	0.0	0.0	0.072445986842874	0.0
31	0.0	0.0	0.0	0.12128860377887615	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	410	0.0	35.19512	37
CTTATAC	2045	0.0	34.557457	1
ATTACGG	70	0.0	34.357143	2
GATTACG	75	0.0	32.066666	1
TTATACA	2265	0.0	31.28256	2
TATACAC	2410	0.0	29.93776	3
GCCTAGT	50	2.7140413E-7	29.599998	1
CCGTCTT	1700	0.0	27.42353	37
GCCGTCT	1735	0.0	26.976946	36
CGTTACT	35	8.860261E-4	26.428572	1
GGTATCA	630	0.0	26.134922	1
GCGGTAT	55	1.899043E-5	23.545454	6
TGCGGTA	55	1.899043E-5	23.545454	5
TATGCGG	55	1.899043E-5	23.545454	3
CGCCGTT	55	1.899043E-5	23.545454	25
TTACCGG	55	1.899043E-5	23.545454	31
TACCGGG	55	1.899043E-5	23.545454	32
ACTGTTC	40	0.0019292169	23.125002	8
GTATCAA	1580	0.0	22.129745	1
TTACGGG	110	7.4578566E-11	21.863636	3
>>END_MODULE