FastQCFastQC Report
Fri 10 Feb 2017
ERR1630941.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630941.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726128
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT77821.071711874490448No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG47380.6525020382081397No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT28040.38615781239671243Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC22580.3109644580569817No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19230.26482934138333736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15180.20905405107639424No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG10560.1454289050966221No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC9960.13716589912522312No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC9330.12848974285525416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG352.3871056E-531.7142851
CTTATAC10200.031.377451
TATCGCC250.005495027629.59999833
GGTATCA3950.029.0379731
TTATACA11400.028.5614032
TATACAC11900.027.2058833
CCGTCTT4400.025.22727237
GCCGTCT4400.024.80681836
CCGATGT551.9014074E-523.54545414
GTATCAA12250.023.2571431
TACCGCG753.736841E-722.28
AACGGCC957.1340764E-921.42105337
GGTACCG806.9528505E-720.81256
GGCCGCA806.9528505E-720.812533
TGCCGTC5250.020.79047635
TAGACGC450.00382447120.55555730
TCGCCCG555.140586E-420.18181810
TCGAGCT656.897655E-519.92307727
CGTCTTC1403.6379788E-1219.82142837
GGCCGTT851.244016E-619.58823614