FastQCFastQC Report
Fri 10 Feb 2017
ERR1630940.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630940.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188390
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT76074.03790010085461RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG73973.9264292159881102No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT65753.49010032379638No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC40672.15881947024789No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38502.043632889219173No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34071.8084824035246032No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC23621.253782047879399RNA PCR Primer, Index 46 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC23351.239450076967992No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA22301.1837146345347418No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12590.6682944954615425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12440.6603322893996497No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11950.6343224162641329No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11520.6114974255533734No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG9900.5255056000849302No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8760.46499283401454433No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8700.4618079515897871No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8620.4575614416901109No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8550.45384574552789425No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7940.4214661075428632No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7730.41031901905621315No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA6950.36891554753437017No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6400.33972079197409627No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT5750.30521789903922714No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT5740.30468708530176764No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5630.2988481341897128No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5630.2988481341897128No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5580.29619406550241517No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5550.2946016242900366No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5460.28982430065290093No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5440.2887626731779819No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5270.2797388396411699No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5130.27230744731673656No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5110.2712458198418175No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5000.2654068687297627No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.22294176973300067No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4170.2213493285206221No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4150.22028770104570305No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4120.21869525983332447No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3930.20860979882159353No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3920.20807898508413397No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3720.19746271033494345No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3710.19693189659748395No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3630.19268538669780774No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3540.187908063060672No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3540.187908063060672No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3490.18525399437337436No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3350.17782260204894101No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3300.1751685333616434No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3200.16986039598704814No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.16826795477466958No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3160.16773714103721005No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3080.16349063113753384No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3050.16189818992515526No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2980.15818249376293858No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2980.15818249376293858No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2940.15605923881310046No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2930.15552842507564096No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2910.1544667976007219No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2910.1544667976007219No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2880.15287435638834335No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2830.1502202877010457No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT2770.14703540527628856No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2710.14385052285153138No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2710.14385052285153138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2700.14331970911407185No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2680.14225808163915282No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT2670.1417272679016933No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2670.1417272679016933No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA2600.13801157173947662No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2600.13801157173947662No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2570.13641913052709803No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2520.13376506183980041No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA2440.1295185519401242No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2410.12792611072774562No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2340.12421041456552896No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT2210.11730983597855513No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2200.11677902224109558No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2170.11518658102871702No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2150.11412495355379797No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2130.11306332607887892No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG2070.10987844365412176No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2040.1082860024417432No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA2020.10722437496682415No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC1950.10350867880460746No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTG1930.10244705132968841No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA1910.10138542385476935No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1900.10085461011730984No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG1890.10032379637985031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGAGT308.2751085E-637.03
AAGGCTT200.00183803237.031
GATTACG551.2732926E-1137.01
TGTAAGG200.00183803237.03
ACTCAGA251.2297105E-436.9999961
CGTCTTC3000.034.53333337
CTTATAC7800.034.391021
TTACGGG651.8189894E-1234.1538473
ATTACGG603.45608E-1133.9166682
GGTATCA2800.033.0357131
TTATACA8450.031.5266282
CTCAGAG303.5881615E-430.8333342
TATACAC8850.030.1016943
GAAGCCA250.005484827329.59999833
TTTTCCA250.005484827329.59999820
CGGAAGC250.005484827329.59999831
GGAAGCC250.005484827329.59999832
AAGCCAC250.005484827329.59999834
CCAGGCA250.005484827329.59999824
TAGGACC453.984909E-628.7777774
Produced by FastQC (version 0.11.5)