FastQCFastQC Report
Fri 10 Feb 2017
ERR1630939.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630939.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922904
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT190962.0691209486577153No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG137991.495171762176781No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT129501.4031795289650928RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC70230.7609675545885596No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA56540.6126314329551069No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC55870.6053717396392257RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC37330.4044841066893198RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23860.25853176495063407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22510.2439040246872914No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC16770.18170904016019002No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG15690.17000684794951587No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA15560.16859825073897175No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA14620.15841300937042205No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG13850.15006977973873772No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA12220.1324081377911462No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT11550.12514844447526502No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG11400.1235231400015603No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC10110.10954552152769952No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT9710.10521137626448689No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAC200.001841748237.034
CTTATAC23650.032.7758981
CGTCTTC7400.030.7537
TTATACA25750.030.3184472
GATTACG551.8550054E-830.2727281
TATACAC26500.029.9490593
GCGATTG250.0054957929.59999825
TCGACGG250.0054957929.59999836
GGTATCA4600.025.3369581
CCGTCTT22200.024.83333437
GCCGTCT22750.024.39560336
GCTTTAT8900.024.1123581
GTATCAA13200.023.4053021
TGCCGTC26350.021.13282835
TTATTGA10050.020.8009974
ATTGAGC10100.020.3316846
TATTGAG10200.020.3137255
ATGCCGT27750.020.034
CTTTATT12250.019.1795922
AGCCTGT10850.019.09677510
Produced by FastQC (version 0.11.5)