FastQCFastQC Report
Fri 10 Feb 2017
ERR1630938.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630938.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342814
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT239556.987754292415128No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG192945.628124872379774No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT142404.153856026883383Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC107463.134644442759047No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC54961.6032017362184743Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC50031.4593919734899974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48531.4156364675888382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46331.3514617256004713No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG23930.6980461708098269No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23110.6741264942505265No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA18720.5460687136464671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17950.5236075539505388No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT15340.44747297368252176No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14770.4308458814400812No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13030.3800894945947365No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12080.352377674190669No Hit
ACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT10830.31591475260636964No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10250.2989959569912547No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9930.2896614490656741No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9810.28616100859358135No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9290.27099243321451283No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8430.2459059431645149No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8120.2368631386116086No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6820.1989417001639373No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6420.1872735652569615No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6380.18610675176626393No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6300.18377312478486876No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6070.1770639472133577No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5960.17385521011393934No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.17356350674126494No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5680.1656875156790563No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5480.1598534482255684No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5470.15956174485289398No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5430.15839493136219643No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5390.15722811787149885No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5360.15635300775347563No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5320.15518619426277805No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5300.15460278751742929No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5080.14818531331859258No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4730.13797569527498876No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4670.1362254750389424No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4620.13476695817557044No Hit
ATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT4600.13418355143022162Illumina PCR Primer Index 10 (95% over 21bp)
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4450.12980800084010571No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4400.12834948397673374No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4370.12747437385871055No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4260.12426563675929223No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4210.12280711989592022No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4210.12280711989592022No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4200.12251541652324584No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4180.12193200977789706No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4140.12076519628719948No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3940.11493112883371158No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA3840.11201409510696762No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3810.11113898498894444No Hit
TCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT3810.11113898498894444Illumina PCR Primer Index 10 (95% over 24bp)
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3790.11055557824359566No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.11055557824359566No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3770.10997217149824685No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3760.10968046812557246No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3690.1076385445168517No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3660.10676343439882852No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3630.10588832428080534No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3580.10442980741743337No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3560.10384640067208457No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3540.10326299392673578No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3510.1023878838087126No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3460.10092936694534062No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3450.10063766357266624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTTT355.615948E-737.03
CAGGGAA200.001840134737.033
TTACGGG600.037.03
GATTACG600.037.01
ACGTTTA251.2318193E-436.99999626
GTTTTAG251.2318193E-436.9999961
TCACGTT251.2318193E-436.99999624
CTTATAC24750.036.103031
ATTACGG651.8189894E-1234.1538472
CGTCTTC7100.033.0915537
TTATACA27100.032.9723242
TCGCCGA352.383457E-531.7142851
TATACAC28500.031.4175433
TGATACA601.2641976E-930.8333342
GATACAC601.2641976E-930.8333343
TTTACAC303.5942564E-430.8333342
ATCACGT303.5942564E-430.83333423
TTTTTGA303.5942564E-430.83333417
TTTTGAT303.5942564E-430.83333418
GACAGGT250.005491031329.5999987
Produced by FastQC (version 0.11.5)