##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630938.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	342814
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.205472938678117	31.0	31.0	34.0	28.0	34.0
2	31.235439626152957	31.0	31.0	34.0	28.0	34.0
3	30.693973991727294	31.0	31.0	34.0	26.0	34.0
4	34.85070037979779	37.0	35.0	37.0	32.0	37.0
5	35.05919536541681	35.0	35.0	37.0	32.0	37.0
6	35.25808455897367	37.0	35.0	37.0	32.0	37.0
7	35.257320296137266	37.0	35.0	37.0	32.0	37.0
8	35.26692608819943	37.0	35.0	37.0	32.0	37.0
9	37.10531366863664	39.0	37.0	39.0	33.0	39.0
10	36.91248898819768	39.0	37.0	39.0	33.0	39.0
11	36.95249610575998	39.0	37.0	39.0	33.0	39.0
12	36.82531343527394	39.0	37.0	39.0	32.0	39.0
13	36.91132217470698	39.0	37.0	39.0	33.0	39.0
14	37.732945562316594	39.0	37.0	41.0	32.0	41.0
15	37.734797878733076	39.0	37.0	41.0	32.0	41.0
16	37.72641140676868	39.0	37.0	41.0	32.0	41.0
17	37.74966016557084	39.0	37.0	41.0	32.0	41.0
18	37.90702830106122	40.0	37.0	41.0	33.0	41.0
19	37.86454170483119	40.0	37.0	41.0	33.0	41.0
20	37.90138675783369	40.0	37.0	41.0	33.0	41.0
21	37.7603131727409	39.0	37.0	41.0	32.0	41.0
22	37.50271284136587	39.0	36.0	41.0	32.0	41.0
23	37.65927587554767	39.0	37.0	41.0	32.0	41.0
24	37.480910931292186	39.0	36.0	41.0	32.0	41.0
25	37.468145991703956	39.0	36.0	41.0	32.0	41.0
26	37.28694277363235	39.0	36.0	41.0	31.0	41.0
27	37.05944039624986	39.0	36.0	40.0	31.0	41.0
28	37.14966716645178	39.0	36.0	40.0	31.0	41.0
29	36.96047419300262	39.0	35.0	40.0	31.0	41.0
30	36.84561015594462	39.0	35.0	40.0	31.0	41.0
31	36.65573459660341	39.0	35.0	40.0	30.0	41.0
32	36.33888347617075	38.0	35.0	40.0	30.0	41.0
33	36.34058410683345	38.0	35.0	40.0	30.0	41.0
34	36.196786012239876	38.0	35.0	40.0	30.0	41.0
35	36.255018756526866	38.0	35.0	40.0	30.0	41.0
36	36.17589713372266	38.0	35.0	40.0	29.0	41.0
37	36.01072593301324	38.0	35.0	40.0	29.0	41.0
38	35.89975905301417	38.0	35.0	40.0	28.0	41.0
39	35.65653386384454	38.0	34.0	40.0	27.0	41.0
40	35.29233053492565	38.0	34.0	40.0	26.0	41.0
41	35.18998057255538	38.0	34.0	40.0	26.0	41.0
42	34.98022251133268	38.0	34.0	40.0	26.0	41.0
43	33.90674826582345	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	16.0
10	19.0
11	21.0
12	13.0
13	17.0
14	11.0
15	17.0
16	14.0
17	20.0
18	18.0
19	37.0
20	93.0
21	168.0
22	322.0
23	545.0
24	999.0
25	1618.0
26	2509.0
27	3661.0
28	5124.0
29	6942.0
30	8732.0
31	10929.0
32	13246.0
33	16724.0
34	21338.0
35	27470.0
36	36632.0
37	52018.0
38	73931.0
39	59608.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.31135834592519	23.219296761509156	16.715770067733523	26.75357482483213
2	12.060184239850182	20.842789384330864	43.81676360942085	23.280262766398106
3	13.558956168651223	30.92259942709458	36.90076834668363	18.617676057570577
4	9.202658001131809	21.776531880261597	28.665690432712783	40.35511968589381
5	13.022805369675684	37.41795842643533	31.50571446907069	18.053521734818297
6	24.732653858943916	38.451171772448035	15.529120747694083	21.287053620913966
7	21.119907588371536	33.725285431750166	15.674389027285935	29.48041795259237
8	26.587012199035044	29.834545847019083	24.174333603645128	19.404108350300746
9	20.511122649600075	11.11769064273921	14.632132876720322	53.7390538309404
10	15.592712082937101	27.723196835601815	27.226134288564648	29.45795679289644
11	30.011318090859767	19.514080521798995	23.6562684137754	26.818332973565838
12	19.11765563833449	26.363275712193783	21.322058025634895	33.19701062383683
13	35.49884193761048	16.347057004673086	24.974184251518317	23.179916806198115
14	22.68985514010513	24.484414288798007	22.132701698297037	30.693028872799825
15	28.315646385503506	23.943887939232354	18.417859247288618	29.322606427975522
16	29.36636193387668	21.36260479443663	17.96542731627063	31.305605955416056
17	18.355726428908973	34.05140980240014	18.892168931257185	28.70069483743371
18	28.150250573197127	16.27617308511321	21.396734089039537	34.176842252650125
19	26.08236536430834	24.141079419160246	21.56504693507266	28.21150828145875
20	26.790912856534447	19.01089220393566	21.468201415344765	32.729993524185126
21	32.91872560630546	19.526915470196666	21.476077406406972	26.0782815170909
22	21.156662213328513	39.09233578558635	21.209752227155253	18.541249773929884
23	30.808251705006214	17.37647820684103	21.87483591685287	29.94043417129989
24	26.244260736142632	24.47799681459917	21.86608481567264	27.411657633585563
25	23.831873844125386	26.528088117754816	23.330144043125426	26.309893994994372
26	29.20971722275053	20.438196806431478	25.68856581119791	24.663520159620088
27	24.907967585921227	32.20696937697994	24.035774501624786	18.849288535474045
28	26.00127182670486	18.590255940539187	27.52921409277334	27.879258139982614
29	19.05085556599205	25.21338101711132	22.13911917249587	33.59664424440075
30	31.380282018820704	18.69760278168336	28.662189992240688	21.259925207255247
31	29.650189315488866	17.403898323872422	24.169666349682334	28.77624601095638
32	20.69285385077622	28.36202722175874	25.83062535369034	25.1144935737747
33	24.36131546552941	19.355393887064125	36.18492827014066	20.098362377265804
34	17.103443850017793	28.103578033569228	24.418197623200918	30.37478049321206
35	18.191205726720614	19.356852403927494	37.181095287823716	25.27084658152818
36	22.379482751579573	19.82182757997048	30.2729760161487	27.52571365230125
37	21.823496123262174	25.899175646268823	29.161586166259255	23.115742064209748
38	20.39589981739369	18.17049478726073	31.696780178172418	29.736825217173163
39	27.847170768988434	20.0391465926129	25.065779110538077	27.04790352786059
40	18.281925475622348	18.820409901579282	41.53301790475302	21.364646718045353
41	23.960515031474795	22.085445751923785	23.469869958636462	30.48416925796496
42	24.75190628154043	18.392481053865943	34.91864392936111	21.93696873523252
43	24.325144247317787	18.579171212377556	32.80758662131652	24.28809791898814
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	5.5
2	10.0
3	71.5
4	133.0
5	133.0
6	213.0
7	293.0
8	345.5
9	398.0
10	570.0
11	742.0
12	742.0
13	1206.5
14	1671.0
15	4422.5
16	7174.0
17	7292.5
18	7411.0
19	7411.0
20	5983.0
21	4555.0
22	3498.5
23	2442.0
24	2439.0
25	2436.0
26	2436.0
27	2555.0
28	2674.0
29	2618.5
30	2563.0
31	2672.0
32	2781.0
33	2781.0
34	3222.5
35	3664.0
36	3814.0
37	3964.0
38	4406.0
39	4848.0
40	4848.0
41	5986.5
42	7125.0
43	9212.0
44	11299.0
45	15076.0
46	18853.0
47	18853.0
48	23698.5
49	28544.0
50	27757.0
51	26970.0
52	30602.5
53	34235.0
54	34235.0
55	52884.0
56	71533.0
57	47211.0
58	22889.0
59	31551.5
60	40214.0
61	40214.0
62	31438.0
63	22662.0
64	13995.5
65	5329.0
66	4186.0
67	3043.0
68	3043.0
69	2273.5
70	1504.0
71	1052.0
72	600.0
73	373.5
74	147.0
75	147.0
76	102.5
77	58.0
78	43.5
79	29.0
80	19.5
81	10.0
82	10.0
83	7.5
84	5.0
85	5.0
86	5.0
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	342814.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.28429994107592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.81835518876682	12.240456924162956
2	11.098172244443319	3.836482757413641
3	4.538166843872884	2.3531711073643433
4	2.597336843704116	1.7957259621835748
5	1.7231195044976624	1.4891457175027858
6	1.1779994261893914	1.2216537247603658
7	0.9721026783454002	1.1761479986231602
8	0.8033348522437682	1.1108064431440956
9	0.5653722174404672	0.8794856686133005
>10	4.5533559482220305	16.16153365965217
>50	0.6143148870099404	7.421517207581954
>100	0.47254991308456956	15.887040785965567
>500	0.03544124348134271	4.025214839533975
>1k	0.02025213913219584	7.4346438593523025
>5k	0.0033753565220326393	3.0625937097084717
>10k+	0.0067507130440652785	19.904379634437333
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT	23955	6.987754292415128	No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG	19294	5.628124872379774	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	14240	4.153856026883383	Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC	10746	3.134644442759047	No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC	5496	1.6032017362184743	Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC	5003	1.4593919734899974	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4853	1.4156364675888382	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4633	1.3514617256004713	No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG	2393	0.6980461708098269	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2311	0.6741264942505265	No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA	1872	0.5460687136464671	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1795	0.5236075539505388	No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT	1534	0.44747297368252176	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1477	0.4308458814400812	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1303	0.3800894945947365	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1208	0.352377674190669	No Hit
ACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT	1083	0.31591475260636964	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1025	0.2989959569912547	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	993	0.2896614490656741	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	981	0.28616100859358135	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	929	0.27099243321451283	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	843	0.2459059431645149	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	812	0.2368631386116086	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	682	0.1989417001639373	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	642	0.1872735652569615	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	638	0.18610675176626393	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	630	0.18377312478486876	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	607	0.1770639472133577	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	596	0.17385521011393934	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	595	0.17356350674126494	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	568	0.1656875156790563	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	548	0.1598534482255684	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	547	0.15956174485289398	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	543	0.15839493136219643	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	539	0.15722811787149885	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	536	0.15635300775347563	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	532	0.15518619426277805	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	530	0.15460278751742929	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	508	0.14818531331859258	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	473	0.13797569527498876	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	467	0.1362254750389424	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	462	0.13476695817557044	No Hit
ATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT	460	0.13418355143022162	Illumina PCR Primer Index 10 (95% over 21bp)
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	445	0.12980800084010571	No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA	440	0.12834948397673374	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	437	0.12747437385871055	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	426	0.12426563675929223	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	421	0.12280711989592022	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	421	0.12280711989592022	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	420	0.12251541652324584	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	418	0.12193200977789706	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA	414	0.12076519628719948	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	394	0.11493112883371158	No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA	384	0.11201409510696762	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	381	0.11113898498894444	No Hit
TCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT	381	0.11113898498894444	Illumina PCR Primer Index 10 (95% over 24bp)
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	379	0.11055557824359566	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	379	0.11055557824359566	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	377	0.10997217149824685	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	376	0.10968046812557246	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	369	0.1076385445168517	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	366	0.10676343439882852	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	363	0.10588832428080534	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	358	0.10442980741743337	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	356	0.10384640067208457	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	354	0.10326299392673578	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	351	0.1023878838087126	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	346	0.10092936694534062	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	345	0.10063766357266624	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.9170337267439485E-4	0.0
11	0.0	0.0	0.0	2.9170337267439485E-4	0.0
12	0.0	0.0	0.0	5.834067453487897E-4	0.0
13	0.0	0.0	0.0	8.751101180231846E-4	0.0
14	0.0	0.0	0.0	8.751101180231846E-4	0.0
15	0.0	0.0	0.0	0.0011668134906975794	0.0
16	0.0	0.0	0.0	0.0011668134906975794	0.0
17	0.0	0.0	0.0	0.0011668134906975794	0.0
18	0.0	0.0	0.0	0.0014585168633719744	0.0
19	0.0	0.0	0.0	0.0014585168633719744	0.0
20	0.0	0.0	0.0	0.002041923608720764	0.0
21	0.0	0.0	0.0	0.0035004404720927382	0.0
22	0.0	0.0	0.0	0.004958957335464712	0.0
23	0.0	0.0	0.0	0.005542364080813503	0.0
24	0.0	0.0	0.0	0.0070008809441854765	0.0
25	0.0	0.0	0.0	0.00787599106220866	0.0
26	0.0	0.0	0.0	0.009042804552906241	0.0
27	0.0	0.0	0.0	0.01079302478895261	0.0
28	0.0	0.0	0.0	0.025378193422672352	0.0
29	0.0	0.0	0.0	0.06680007234243643	0.0
30	0.0	0.0	0.0	0.12018178954185069	0.0
31	0.0	0.0	0.0	0.17910587082207843	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACTTT	35	5.615948E-7	37.0	3
CAGGGAA	20	0.0018401347	37.0	33
TTACGGG	60	0.0	37.0	3
GATTACG	60	0.0	37.0	1
ACGTTTA	25	1.2318193E-4	36.999996	26
GTTTTAG	25	1.2318193E-4	36.999996	1
TCACGTT	25	1.2318193E-4	36.999996	24
CTTATAC	2475	0.0	36.10303	1
ATTACGG	65	1.8189894E-12	34.153847	2
CGTCTTC	710	0.0	33.09155	37
TTATACA	2710	0.0	32.972324	2
TCGCCGA	35	2.383457E-5	31.714285	1
TATACAC	2850	0.0	31.417543	3
TGATACA	60	1.2641976E-9	30.833334	2
GATACAC	60	1.2641976E-9	30.833334	3
TTTACAC	30	3.5942564E-4	30.833334	2
ATCACGT	30	3.5942564E-4	30.833334	23
TTTTTGA	30	3.5942564E-4	30.833334	17
TTTTGAT	30	3.5942564E-4	30.833334	18
GACAGGT	25	0.0054910313	29.599998	7
>>END_MODULE