FastQCFastQC Report
Fri 10 Feb 2017
ERR1630937.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630937.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622178
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT134532.16224295941033No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG106781.7162291177122944No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT102541.6480814172150093RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC54710.8793303524071889No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC39810.6398490464143701RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC32090.5157687992825204No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13130.21103285554937654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.20717543854009624No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG12320.1980140731430555No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA11330.18210222797977427No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC9050.1454567663916114No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT8880.14272442934337118No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT7220.11604396169584909RNA PCR Primer, Index 26 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15900.034.440251
CGTCTTC5800.032.85344737
GATTACG352.3865603E-531.7142851
TTATACA18400.029.9619582
CGAACTA250.00549442929.624
TATACAC19200.029.0026053
GCCGTCT18450.025.86991936
CCGTCTT18750.025.45637
GGTATCA2700.025.351851
CCGGTCG951.0913936E-1125.315799
CGGTCGT951.0913936E-1125.3157910
CCCGGTC1054.0017767E-1122.904768
GTATCAA7050.022.8297881
ATTACGG502.7001713E-422.22
TGCCGTC21500.022.19999935
ATGCCGT22400.021.22544534
TCGTGTA1500.020.96666513
CGTGTAG1450.020.41379414
TATGCCG23300.020.40557933
GGTCGTG1202.382876E-1020.04166811
Produced by FastQC (version 0.11.5)