##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630937.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	622178
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.475230561029157	31.0	31.0	34.0	28.0	34.0
2	31.496002751624133	31.0	31.0	34.0	28.0	34.0
3	30.987863601734553	31.0	31.0	34.0	27.0	34.0
4	35.112647827470596	37.0	35.0	37.0	32.0	37.0
5	35.29445432014632	37.0	35.0	37.0	33.0	37.0
6	35.47242911192617	37.0	35.0	37.0	33.0	37.0
7	35.47159494549791	37.0	35.0	37.0	33.0	37.0
8	35.501456174921	37.0	35.0	37.0	33.0	37.0
9	37.28587638907194	39.0	37.0	39.0	34.0	39.0
10	37.117016352233605	39.0	37.0	39.0	33.0	39.0
11	37.165398005072504	39.0	37.0	39.0	33.0	39.0
12	37.10615772335248	39.0	37.0	39.0	33.0	39.0
13	37.181028580245524	39.0	37.0	39.0	33.0	39.0
14	38.3200482820029	40.0	38.0	41.0	34.0	41.0
15	38.308511390630976	40.0	38.0	41.0	33.0	41.0
16	38.29943842437373	40.0	38.0	41.0	33.0	41.0
17	38.248965730064384	40.0	38.0	41.0	33.0	41.0
18	38.301134080600725	40.0	38.0	41.0	33.0	41.0
19	38.335481164554196	40.0	38.0	41.0	34.0	41.0
20	38.342744359331256	40.0	38.0	41.0	34.0	41.0
21	38.32102549431192	40.0	38.0	41.0	34.0	41.0
22	38.23409217297944	40.0	38.0	41.0	34.0	41.0
23	38.22867089482431	40.0	38.0	41.0	34.0	41.0
24	38.15825696183407	40.0	38.0	41.0	33.0	41.0
25	38.15075107123685	40.0	37.0	41.0	33.0	41.0
26	38.039831045134996	40.0	37.0	41.0	33.0	41.0
27	37.969508725798725	40.0	37.0	41.0	33.0	41.0
28	37.99012661971333	40.0	37.0	41.0	33.0	41.0
29	37.9335752791002	40.0	37.0	41.0	33.0	41.0
30	37.87168945221464	40.0	37.0	41.0	33.0	41.0
31	37.83817653468943	40.0	37.0	41.0	33.0	41.0
32	37.766663237851546	40.0	37.0	41.0	33.0	41.0
33	37.687438321509276	40.0	37.0	41.0	33.0	41.0
34	37.62092037969841	40.0	37.0	41.0	32.0	41.0
35	37.622391019933204	40.0	37.0	41.0	32.0	41.0
36	37.59754603987926	40.0	37.0	41.0	32.0	41.0
37	37.56511159185956	40.0	37.0	41.0	32.0	41.0
38	37.53920100035681	40.0	37.0	41.0	32.0	41.0
39	37.46725052959121	40.0	37.0	41.0	32.0	41.0
40	37.37526881374784	39.0	37.0	41.0	31.0	41.0
41	37.33317153611989	39.0	36.0	41.0	31.0	41.0
42	37.29128159465619	39.0	36.0	41.0	31.0	41.0
43	36.63396005644687	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	12.0
10	13.0
11	17.0
12	16.0
13	12.0
14	13.0
15	10.0
16	7.0
17	15.0
18	23.0
19	33.0
20	54.0
21	111.0
22	205.0
23	438.0
24	769.0
25	1322.0
26	2119.0
27	3333.0
28	5056.0
29	7090.0
30	9911.0
31	13411.0
32	18056.0
33	22927.0
34	30590.0
35	41522.0
36	56884.0
37	85128.0
38	149538.0
39	173542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.217770477258924	22.785440822401306	15.644719035388588	25.352069664951188
2	13.245887832742397	21.317693650370153	41.99152011160793	23.444898405279517
3	14.046462587876782	28.56674456506016	34.94000109293482	22.44679175412824
4	11.297088614512246	19.35780435823832	37.87742414550177	31.467682881747667
5	11.908810661900613	38.56179421323158	36.18835767256316	13.341037452304647
6	27.495507716441274	40.311936455483796	17.757940653639313	14.434615174435612
7	23.801227301511783	33.52529340478127	23.24415199508822	19.429327298618723
8	24.62301785019721	35.78879356068521	22.31451449585167	17.273674093265914
9	23.178415180221737	15.244833472093195	19.447489303704085	42.12926204398098
10	14.241744323971597	28.792242734394335	34.00682762810643	22.95918531352764
11	30.202450102703725	23.08856950904725	25.910270051335793	20.79871033691323
12	19.715740511557787	26.604283661588806	29.294992751270538	24.384983075582873
13	28.111408632256364	20.318622644966553	27.441021701185193	24.12894702159189
14	21.30451414225511	22.60623165717849	26.26354515910238	29.825709041464016
15	23.457435010559678	29.403643330365263	22.47957979870712	24.659341860367935
16	24.956202244373795	27.109283838387082	23.667824963274175	24.266688953964945
17	20.099392778272456	31.820315086679372	25.227828692110617	22.85246344293755
18	23.445059130988238	25.578692914246403	26.183343030451095	24.792904924314264
19	22.142377261812534	27.643053917046245	27.383160446045984	22.83140837509523
20	21.17625502669654	26.475703094612797	25.429539456554235	26.91850242213643
21	23.693701802378097	25.473096123617356	26.256473227918697	24.576728846085846
22	21.330873158485193	29.925037529452986	27.372552549270466	21.371536762791354
23	24.262831536955662	25.777028438806898	25.65744851151921	24.302691512718226
24	23.08760515479493	29.234238433374372	26.88941106885811	20.78874534297259
25	23.2054170992867	27.266152130097815	25.79020794692194	23.73822282369354
26	22.844266431792832	26.668252493659374	27.640482305706726	22.846998768841072
27	21.300174548119667	29.22925593640405	26.782207021141858	22.68836249433442
28	21.42618350375616	25.049744606848844	27.933645998412032	25.590425890982964
29	20.811407667902113	26.216452526447416	30.668554657991763	22.30358514765871
30	23.24977739489342	25.25933093101974	27.627624249009124	23.863267425077712
31	21.445309863093843	26.91512718225331	25.968774209309874	25.67078874534297
32	24.164145951801576	25.968131306474994	29.184574189379887	20.683148552343543
33	23.09596289164837	26.216452526447416	27.93686051258643	22.750724069317783
34	21.57694421853553	30.570190524255114	26.26708112469422	21.585784132515133
35	23.903609577966435	25.073049834613247	30.605228728756078	20.41811185866424
36	23.046780824780047	26.155216031425088	24.845622956774427	25.952380187020434
37	21.548335042383368	27.156215745333327	28.27406304948102	23.021386162802283
38	22.106696154476694	25.39225109213119	28.601300592434963	23.899752160957156
39	23.7202215443169	26.083371639627245	26.77111694724018	23.42528986881568
40	20.985152159028445	24.36055276785743	32.544223678754314	22.110071394359814
41	21.992902352702927	26.38842903477783	26.461077055119276	25.15759155739997
42	22.429272651877756	25.101980462182848	30.285706019820697	22.1830408661187
43	22.406449601239515	24.445576667770315	30.50123919521423	22.646734535775938
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	10.5
2	9.0
3	58.0
4	107.0
5	107.0
6	126.0
7	145.0
8	159.0
9	173.0
10	258.0
11	343.0
12	343.0
13	544.5
14	746.0
15	1595.0
16	2444.0
17	2929.0
18	3414.0
19	3414.0
20	3958.0
21	4502.0
22	5808.0
23	7114.0
24	9233.0
25	11352.0
26	11352.0
27	14158.5
28	16965.0
29	20103.5
30	23242.0
31	26258.0
32	29274.0
33	29274.0
34	32156.5
35	35039.0
36	37160.5
37	39282.0
38	41264.5
39	43247.0
40	43247.0
41	44112.0
42	44977.0
43	44624.5
44	44272.0
45	43140.5
46	42009.0
47	42009.0
48	40012.5
49	38016.0
50	38470.0
51	38924.0
52	48113.0
53	57302.0
54	57302.0
55	43941.5
56	30581.0
57	35603.5
58	40626.0
59	33073.5
60	25521.0
61	25521.0
62	19884.5
63	14248.0
64	11421.0
65	8594.0
66	7062.5
67	5531.0
68	5531.0
69	4661.5
70	3792.0
71	3263.5
72	2735.0
73	3146.0
74	3557.0
75	3557.0
76	3110.5
77	2664.0
78	1893.0
79	1122.0
80	662.0
81	202.0
82	202.0
83	132.5
84	63.0
85	42.0
86	21.0
87	12.5
88	4.0
89	4.0
90	4.0
91	4.0
92	3.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	622178.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.62885085090803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8534721413692	44.169322703889044
2	11.585491422830362	12.658041659445482
3	3.2345308055616027	5.300961028490765
4	1.3422706264420912	2.9330680741383954
5	0.6888645017528033	1.881593806136948
6	0.4399780171965715	1.4421296087465851
7	0.29061479957063757	1.1113166778567622
8	0.2048759592551407	0.8953710576868634
9	0.15656241934469287	0.769754254971469
>10	1.0480847067052181	10.991586247387016
>50	0.09710792005579422	3.718585515977063
>100	0.053719267739647684	5.151390074089222
>500	0.0014758040587814502	0.5901492322263034
>1k	0.0017709648705377403	1.9602306601571509
>5k	2.9516081175629005E-4	0.8821602321065865
>10k+	8.854824352688701E-4	5.5443391666943835
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	13453	2.16224295941033	No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG	10678	1.7162291177122944	No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	10254	1.6480814172150093	RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC	5471	0.8793303524071889	No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC	3981	0.6398490464143701	RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	3209	0.5157687992825204	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1313	0.21103285554937654	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1289	0.20717543854009624	No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG	1232	0.1980140731430555	No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA	1133	0.18210222797977427	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	905	0.1454567663916114	No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT	888	0.14272442934337118	No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT	722	0.11604396169584909	RNA PCR Primer, Index 26 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.607257087200126E-4	0.0
11	0.0	0.0	0.0	1.607257087200126E-4	0.0
12	0.0	0.0	0.0	1.607257087200126E-4	0.0
13	0.0	0.0	0.0	1.607257087200126E-4	0.0
14	0.0	0.0	0.0	1.607257087200126E-4	0.0
15	0.0	0.0	0.0	1.607257087200126E-4	0.0
16	0.0	0.0	0.0	1.607257087200126E-4	0.0
17	0.0	0.0	0.0	1.607257087200126E-4	0.0
18	0.0	0.0	0.0	1.607257087200126E-4	0.0
19	0.0	0.0	0.0	1.607257087200126E-4	0.0
20	0.0	0.0	0.0	3.214514174400252E-4	0.0
21	0.0	0.0	0.0	6.429028348800504E-4	1.607257087200126E-4
22	0.0	0.0	0.0	8.03628543600063E-4	3.214514174400252E-4
23	0.0	0.0	0.0	0.0012858056697601008	4.821771261600378E-4
24	0.0	0.0	0.0	0.0012858056697601008	4.821771261600378E-4
25	0.0	0.0	0.0	0.0012858056697601008	4.821771261600378E-4
26	0.0	0.0	0.0	0.0012858056697601008	4.821771261600378E-4
27	0.0	0.0	0.0	0.0019287085046401512	4.821771261600378E-4
28	0.0	0.0	0.0	0.005625399805200441	4.821771261600378E-4
29	0.0	0.0	0.0	0.012858056697601008	4.821771261600378E-4
30	0.0	0.0	0.0	0.02893062756960227	4.821771261600378E-4
31	0.0	0.0	0.0	0.06991568329320548	4.821771261600378E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1590	0.0	34.44025	1
CGTCTTC	580	0.0	32.853447	37
GATTACG	35	2.3865603E-5	31.714285	1
TTATACA	1840	0.0	29.961958	2
CGAACTA	25	0.005494429	29.6	24
TATACAC	1920	0.0	29.002605	3
GCCGTCT	1845	0.0	25.869919	36
CCGTCTT	1875	0.0	25.456	37
GGTATCA	270	0.0	25.35185	1
CCGGTCG	95	1.0913936E-11	25.31579	9
CGGTCGT	95	1.0913936E-11	25.31579	10
CCCGGTC	105	4.0017767E-11	22.90476	8
GTATCAA	705	0.0	22.829788	1
ATTACGG	50	2.7001713E-4	22.2	2
TGCCGTC	2150	0.0	22.199999	35
ATGCCGT	2240	0.0	21.225445	34
TCGTGTA	150	0.0	20.966665	13
CGTGTAG	145	0.0	20.413794	14
TATGCCG	2330	0.0	20.405579	33
GGTCGTG	120	2.382876E-10	20.041668	11
>>END_MODULE