FastQCFastQC Report
Fri 10 Feb 2017
ERR1630936.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630936.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591574
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG149802.5322275826861897No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT117971.9941714815052722TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT106531.800789081332175No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC83021.403374725731692No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC45450.7682893433450422RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC39690.670921981020126No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG35240.5956989320017445No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG20340.3438284982098605No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA18240.3083299806955681No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG17520.29615906040495354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.285340464591074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16620.2809454100416854No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC13550.22904995824698177No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA12590.21282206452616242No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT12090.20437003654656896No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT11260.19033967010044392No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC10380.1754641008563595No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG10270.17360465470084893No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT10160.17174520854533837No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC9170.15501019314574338No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG8720.1474033679641093No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT8650.14622008404696624No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT7940.13421820431594356No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC7680.12982314976655498No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA7600.12847082528982004No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA7330.12390673018083959No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC6990.11815935115471607No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC6860.11596182388002177No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG6560.11089060709226572No Hit
GATCAGAGGTCTGATATGGACAATACTAGTTAAGAAAGCTAAC6430.10869307981757144No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT6380.10784787701961207No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG6290.10632651198328527No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG6250.10565034974491778No Hit
GGTCTGATATGGACAATACTAGTTAAGAAAGCTAACAGGATGT6110.10328378191063164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6110.10328378191063164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGCGG251.2329016E-436.9999968
CTTATAC14550.033.439861
CGTCTTC6900.031.36956437
GATTACG303.597385E-430.8333341
GTTGGCG250.00549421429.5999987
TTATACA16500.029.487882
TATACAC16900.029.3372763
GGTATCA2850.026.6140371
GCAACGC358.8642875E-426.428573
GTATCAA8700.025.0919551
CCGTCTT20400.024.5759837
GCCGTCT21050.023.72921836
TTACGGG400.001930084823.1250023
TTAGACT502.6999548E-422.1999994
TTAACGG753.7344216E-722.19999935
CGTCTTT705.0950184E-621.14285737
GTATAGA806.948376E-720.81251
TGCCGTC24650.020.33874335
GCAATAC555.1390403E-420.1818183
GCGTCTT555.1390403E-420.18181836
Produced by FastQC (version 0.11.5)