##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630936.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	591574
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.753596337905318	31.0	30.0	33.0	27.0	34.0
2	30.742779432497034	31.0	31.0	34.0	27.0	34.0
3	30.166373099561508	31.0	30.0	34.0	26.0	34.0
4	34.43809227586067	35.0	35.0	37.0	30.0	37.0
5	34.4541900083506	35.0	35.0	37.0	30.0	37.0
6	34.68960941488301	35.0	35.0	37.0	32.0	37.0
7	34.718229672027505	35.0	35.0	37.0	32.0	37.0
8	34.765872063342876	35.0	35.0	37.0	32.0	37.0
9	36.47911672926802	38.0	35.0	39.0	32.0	39.0
10	36.18258747003756	37.0	35.0	39.0	32.0	39.0
11	36.292560186891244	38.0	35.0	39.0	32.0	39.0
12	36.168073647591	37.0	35.0	39.0	32.0	39.0
13	36.325947387816235	38.0	35.0	39.0	32.0	39.0
14	37.25015298170643	39.0	36.0	40.0	32.0	41.0
15	37.260936079002796	39.0	36.0	40.0	32.0	41.0
16	37.26306429964806	39.0	36.0	40.0	32.0	41.0
17	37.161127770997375	39.0	36.0	40.0	32.0	41.0
18	37.21792201820905	39.0	36.0	40.0	32.0	41.0
19	37.254985851305165	39.0	36.0	40.0	32.0	41.0
20	37.238404662814794	39.0	36.0	40.0	32.0	41.0
21	37.17605067159814	39.0	36.0	40.0	31.0	41.0
22	36.9963943648639	39.0	36.0	40.0	31.0	41.0
23	37.02283399878967	39.0	36.0	40.0	31.0	41.0
24	36.92286848306382	39.0	36.0	40.0	31.0	41.0
25	36.84443704422439	39.0	36.0	40.0	31.0	41.0
26	36.726460256874034	38.0	36.0	40.0	31.0	41.0
27	36.58744637188247	38.0	35.0	40.0	30.0	41.0
28	36.5681740576834	38.0	35.0	40.0	30.0	41.0
29	36.39783357618827	38.0	35.0	40.0	30.0	41.0
30	36.286664052172675	38.0	35.0	40.0	30.0	41.0
31	36.27349410217487	38.0	35.0	40.0	30.0	41.0
32	36.01707816773557	38.0	35.0	40.0	29.0	41.0
33	36.00933103888947	38.0	34.0	40.0	29.0	41.0
34	35.96115616981138	38.0	34.0	40.0	29.0	41.0
35	35.88378968649738	38.0	34.0	40.0	29.0	41.0
36	35.96468066547887	38.0	34.0	40.0	29.0	41.0
37	35.942152630102065	38.0	34.0	40.0	29.0	41.0
38	35.901028442764556	38.0	34.0	40.0	29.0	41.0
39	35.74903731401312	38.0	34.0	40.0	28.0	41.0
40	35.60858320345384	38.0	34.0	40.0	28.0	41.0
41	35.57152613198011	38.0	34.0	40.0	28.0	41.0
42	35.5205857593471	38.0	34.0	40.0	28.0	41.0
43	34.675562144380926	37.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	13.0
10	13.0
11	19.0
12	17.0
13	22.0
14	10.0
15	9.0
16	18.0
17	21.0
18	41.0
19	75.0
20	136.0
21	303.0
22	581.0
23	990.0
24	1791.0
25	2786.0
26	4334.0
27	6259.0
28	8950.0
29	12101.0
30	15618.0
31	20569.0
32	26393.0
33	33995.0
34	43780.0
35	58594.0
36	79949.0
37	106527.0
38	123049.0
39	44609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.72710430140608	24.45425255335765	15.689837619638455	23.128805525597812
2	14.64888585367173	22.69437128744671	39.98248739802629	22.674255460855278
3	15.672595482560087	28.915571002106244	33.50806492509813	21.903768590235543
4	11.344819075889069	19.574389679059593	35.89880555940592	33.181985685645415
5	12.487026137051323	38.36730485112598	35.130854297180065	14.014814714642633
6	27.85484149066727	39.17937569940532	17.29623005743998	15.669552752487432
7	25.011410237772452	33.54728233492344	21.46679874369056	19.974508683613546
8	24.98757551887	34.05829194656966	23.074543505968823	17.879589028591518
9	22.835182073586736	14.40360800170393	18.84819143505293	43.91301848965641
10	14.381801769516578	28.285556836507354	33.83059431279941	23.50204708117666
11	31.15163952438748	23.109196820685156	23.861089229749783	21.878074425177577
12	19.637441807787358	26.543931951032327	28.47910827724004	25.339517963940267
13	29.831263713415396	20.071030843140502	26.05895458556326	24.03875085788084
14	21.82837650065757	23.460125022397875	24.94683674400836	29.764661732936197
15	24.122594975438407	29.43908961516226	22.18167127020457	24.25664413919476
16	26.16308357027185	26.946755604539753	21.939943269988202	24.950217555200194
17	21.41321288629994	31.00119342635072	23.289563097769676	24.296030589579665
18	23.917886857772654	24.28926896719599	25.176393823934113	26.616450351097242
19	26.395683380270263	27.588095487631303	24.307863428751094	21.708357703347342
20	22.684397894430788	26.534972801373964	24.014747098418795	26.765882205776453
21	27.74259855909827	25.206652084101062	24.479608637296433	22.57114071950424
22	21.646319817977126	30.620176005030647	26.633861528735203	21.09964264825702
23	24.6819501871279	26.74576637918502	25.060770081173278	23.511513352513802
24	25.876897902882817	28.335085720467767	25.436885326265184	20.35113105038423
25	20.964241160023935	28.735542806140906	26.962138295462612	23.338077738372544
26	28.685675841061304	27.47737392109863	23.755269839445276	20.08168039839479
27	20.683802871661026	28.799102056547447	26.38824559564822	24.128849476143305
28	23.91653453329592	25.963108588274668	27.42480230706556	22.69555457136385
29	24.812618539692412	25.584457734788884	28.40912548556901	21.193798239949693
30	21.0856122818109	27.757474128342352	28.166045160875903	22.99086842897085
31	26.278369231913505	25.334615787712107	25.109453762335736	23.277561218038656
32	22.122676114907012	26.438788722966187	31.029085118683376	20.409450043443425
33	20.99179477123741	29.377051729792047	28.65017732354701	20.98097617542353
34	24.043145912430226	28.640711052209866	26.108990591202453	21.20715244415745
35	21.461896567462396	25.1123274518488	32.926058278423326	20.49971770226548
36	22.91784290722716	27.04868706197365	23.817476765375083	26.215993265424103
37	22.152089172275996	26.16071700243757	28.683985435465388	23.003208389821054
38	21.828207460097975	23.69762700862445	27.945616271168106	26.528549260109468
39	25.44973240879417	25.03135702380429	26.494741148191096	23.024169419210445
40	21.386842559003608	24.14440120762576	32.47945311998161	21.989303113389028
41	23.242400781643546	26.428646289390677	25.181634081281462	25.147318847684314
42	21.92794139025718	25.707688302731356	30.26519082988772	22.09917947712374
43	24.536068184200115	23.001517984225135	28.466768316389835	23.99564551518491
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	9.0
2	13.0
3	61.0
4	109.0
5	109.0
6	170.5
7	232.0
8	225.5
9	219.0
10	298.5
11	378.0
12	378.0
13	563.5
14	749.0
15	1792.0
16	2835.0
17	3152.0
18	3469.0
19	3469.0
20	3659.0
21	3849.0
22	4811.0
23	5773.0
24	7507.5
25	9242.0
26	9242.0
27	10829.0
28	12416.0
29	14773.5
30	17131.0
31	19275.0
32	21419.0
33	21419.0
34	28055.0
35	34691.0
36	35700.5
37	36710.0
38	36828.0
39	36946.0
40	36946.0
41	36394.5
42	35843.0
43	37418.0
44	38993.0
45	38196.0
46	37399.0
47	37399.0
48	37779.0
49	38159.0
50	50754.0
51	63349.0
52	51211.5
53	39074.0
54	39074.0
55	48175.0
56	57276.0
57	46242.0
58	35208.0
59	27245.5
60	19283.0
61	19283.0
62	17015.0
63	14747.0
64	11254.5
65	7762.0
66	6534.0
67	5306.0
68	5306.0
69	4518.5
70	3731.0
71	4195.0
72	4659.0
73	4157.5
74	3656.0
75	3656.0
76	2093.5
77	531.0
78	375.5
79	220.0
80	164.0
81	108.0
82	108.0
83	82.0
84	56.0
85	38.0
86	20.0
87	13.0
88	6.0
89	6.0
90	4.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	591574.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.13045050820767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.24066804768376	38.76039735183296
2	10.560192797248924	9.95413287975743
3	2.8124346416304338	3.9765393505479585
4	1.1541854528989328	2.1758912146058567
5	0.6407808409358561	1.510014485516753
6	0.4116079430681054	1.16395606737339
7	0.2859720222121454	0.9434593167721109
8	0.2217692585957912	0.8361668053192675
9	0.16317546996651994	0.6921480070269536
>10	1.2626643984536288	11.56376031034579
>50	0.1387288093198827	4.546356174843192
>100	0.09308487452799924	8.71667244885545
>500	0.007906823318980632	2.49710814693018
>1k	0.005391015899304976	4.916975667418889
>5k	3.5940105995366505E-4	1.4062536857830927
>10k+	0.0010782031798609953	6.340168087070726
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	14980	2.5322275826861897	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	11797	1.9941714815052722	TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	10653	1.800789081332175	No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	8302	1.403374725731692	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	4545	0.7682893433450422	RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	3969	0.670921981020126	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	3524	0.5956989320017445	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	2034	0.3438284982098605	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1824	0.3083299806955681	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1752	0.29615906040495354	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1688	0.285340464591074	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1662	0.2809454100416854	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1355	0.22904995824698177	No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	1259	0.21282206452616242	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	1209	0.20437003654656896	No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT	1126	0.19033967010044392	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	1038	0.1754641008563595	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1027	0.17360465470084893	No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	1016	0.17174520854533837	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	917	0.15501019314574338	No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG	872	0.1474033679641093	No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT	865	0.14622008404696624	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	794	0.13421820431594356	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	768	0.12982314976655498	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	760	0.12847082528982004	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	733	0.12390673018083959	No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC	699	0.11815935115471607	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	686	0.11596182388002177	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	656	0.11089060709226572	No Hit
GATCAGAGGTCTGATATGGACAATACTAGTTAAGAAAGCTAAC	643	0.10869307981757144	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	638	0.10784787701961207	No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG	629	0.10632651198328527	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	625	0.10565034974491778	No Hit
GGTCTGATATGGACAATACTAGTTAAGAAAGCTAACAGGATGT	611	0.10328378191063164	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	611	0.10328378191063164	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	1.6904055959186847E-4	0.0
22	0.0	0.0	0.0	3.3808111918373694E-4	0.0
23	0.0	0.0	0.0	5.071216787756054E-4	0.0
24	0.0	0.0	0.0	6.761622383674739E-4	0.0
25	0.0	0.0	0.0	8.452027979593423E-4	0.0
26	0.0	0.0	0.0	8.452027979593423E-4	0.0
27	0.0	0.0	0.0	0.0010142433575512108	0.0
28	0.0	0.0	0.0	0.004056973430204843	0.0
29	0.0	0.0	0.0	0.011156676933063318	0.0
30	0.0	0.0	0.0	0.020115826591432347	0.0
31	0.0	0.0	0.0	0.050374086758376804	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGCGG	25	1.2329016E-4	36.999996	8
CTTATAC	1455	0.0	33.43986	1
CGTCTTC	690	0.0	31.369564	37
GATTACG	30	3.597385E-4	30.833334	1
GTTGGCG	25	0.005494214	29.599998	7
TTATACA	1650	0.0	29.48788	2
TATACAC	1690	0.0	29.337276	3
GGTATCA	285	0.0	26.614037	1
GCAACGC	35	8.8642875E-4	26.42857	3
GTATCAA	870	0.0	25.091955	1
CCGTCTT	2040	0.0	24.57598	37
GCCGTCT	2105	0.0	23.729218	36
TTACGGG	40	0.0019300848	23.125002	3
TTAGACT	50	2.6999548E-4	22.199999	4
TTAACGG	75	3.7344216E-7	22.199999	35
CGTCTTT	70	5.0950184E-6	21.142857	37
GTATAGA	80	6.948376E-7	20.8125	1
TGCCGTC	2465	0.0	20.338743	35
GCAATAC	55	5.1390403E-4	20.181818	3
GCGTCTT	55	5.1390403E-4	20.181818	36
>>END_MODULE