FastQCFastQC Report
Fri 10 Feb 2017
ERR1630935.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630935.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734068
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT126611.724772091958783No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT110911.5108954483780794RNA PCR Primer, Index 17 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG104591.4247998822997325No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC55940.7620547415225837No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC46290.630595530659285RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC32420.44164845763607735No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20860.28416985892315155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20040.2729992316788091No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG13080.1781851272634143No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA10990.1497136505064926No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT9180.1250565342720293No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT9000.1226044453647346No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC8810.12001612929592353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7850.10693832179035186No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC7810.10639341314428637No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA7680.10462246004457354No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT7640.10407755139850805No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT7420.10108055384514786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG555.002221E-1033.6363641
CTTATAC16200.033.1172831
CGTCTTC6350.032.04724537
TTATACA18550.029.8194072
GGTATCA3250.029.61
TATACAC19300.028.6606223
TTACGGG659.3821654E-825.6153853
TGCACGC601.3363078E-624.66666637
CCGTCTT16950.023.68436637
GCCGTCT17350.023.1383336
AACGGCC400.001930525623.12500237
GTATCAA9800.022.275511
TCGCCGA450.003824510120.5555571
TCGGCGT450.003824510120.55555711
CGCCGAG450.003824510120.5555572
GCACCGC656.897788E-519.92307710
TGCCGTC21000.019.11666735
TCGCCAT1205.164111E-918.513
CCGATCT701.2184361E-418.59
AGCGGAG609.2324836E-418.510
Produced by FastQC (version 0.11.5)