##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630935.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	734068
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.502946593503598	31.0	31.0	34.0	28.0	34.0
2	31.52096536015737	31.0	31.0	34.0	30.0	34.0
3	31.009914612815162	31.0	31.0	34.0	27.0	34.0
4	35.13745048142679	37.0	35.0	37.0	32.0	37.0
5	35.32276301378074	37.0	35.0	37.0	33.0	37.0
6	35.503422026297294	37.0	35.0	37.0	33.0	37.0
7	35.50668058000076	37.0	35.0	37.0	33.0	37.0
8	35.53263185426963	37.0	35.0	37.0	33.0	37.0
9	37.31265359612461	39.0	37.0	39.0	34.0	39.0
10	37.15383179759913	39.0	37.0	39.0	33.0	39.0
11	37.2131846096002	39.0	37.0	39.0	33.0	39.0
12	37.14950522294937	39.0	37.0	39.0	33.0	39.0
13	37.22179825302288	39.0	37.0	39.0	34.0	39.0
14	38.3664565135655	40.0	38.0	41.0	34.0	41.0
15	38.38429273582284	40.0	38.0	41.0	34.0	41.0
16	38.37771705073644	40.0	38.0	41.0	34.0	41.0
17	38.347454186805585	40.0	38.0	41.0	34.0	41.0
18	38.38726385021551	40.0	38.0	41.0	34.0	41.0
19	38.411380144618754	40.0	38.0	41.0	34.0	41.0
20	38.40695139959786	40.0	38.0	41.0	34.0	41.0
21	38.36619223287216	40.0	38.0	41.0	34.0	41.0
22	38.29918345439387	40.0	38.0	41.0	34.0	41.0
23	38.30295285995303	40.0	38.0	41.0	34.0	41.0
24	38.24392154405314	40.0	38.0	41.0	34.0	41.0
25	38.21172834124359	40.0	38.0	41.0	33.0	41.0
26	38.09106513293047	40.0	37.0	41.0	33.0	41.0
27	38.04156426925026	40.0	37.0	41.0	33.0	41.0
28	38.0250467259164	40.0	37.0	41.0	33.0	41.0
29	37.96518442433126	40.0	37.0	41.0	33.0	41.0
30	37.89785687429502	40.0	37.0	41.0	33.0	41.0
31	37.871551409406216	40.0	37.0	41.0	33.0	41.0
32	37.78167553959579	40.0	37.0	41.0	33.0	41.0
33	37.75601851599579	40.0	37.0	41.0	33.0	41.0
34	37.716117580387646	40.0	37.0	41.0	33.0	41.0
35	37.674876169510185	40.0	37.0	41.0	33.0	41.0
36	37.64754082728031	40.0	37.0	41.0	33.0	41.0
37	37.619860830331795	40.0	37.0	41.0	33.0	41.0
38	37.58446628922661	40.0	37.0	41.0	32.0	41.0
39	37.49260014058643	40.0	37.0	41.0	32.0	41.0
40	37.40264253447909	40.0	37.0	41.0	32.0	41.0
41	37.37400077377028	40.0	37.0	41.0	32.0	41.0
42	37.34314804623005	40.0	37.0	41.0	31.0	41.0
43	36.71914727245977	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	6.0
11	10.0
12	6.0
13	7.0
14	7.0
15	3.0
16	6.0
17	10.0
18	15.0
19	37.0
20	82.0
21	138.0
22	282.0
23	527.0
24	863.0
25	1521.0
26	2483.0
27	3960.0
28	5796.0
29	8638.0
30	11505.0
31	15737.0
32	20195.0
33	26437.0
34	35067.0
35	47519.0
36	65830.0
37	98497.0
38	174410.0
39	214466.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.082123182048534	23.04309682481732	15.647596680416529	24.227183312717624
2	13.14496749619926	22.383893590239595	41.64491572987789	22.826223183683254
3	14.131252145577793	28.772811238195917	35.422058991810026	21.673877624416267
4	11.436678890783961	19.556498852967298	38.11663224660386	30.890190009644886
5	11.960608553975927	38.762757673676006	36.104965752491594	13.171668019856472
6	26.755150748976934	40.65495294713841	18.18823324269686	14.401663061187792
7	23.247165112768844	34.34273119111581	23.349607938229155	19.06049575788619
8	24.15634518872911	35.895584605240934	22.607851043772513	17.340219162257448
9	23.213789458197333	15.573216650228588	20.080837197643813	41.13215669393026
10	13.758262177345967	29.23557490586703	34.21767465684378	22.78848825994322
11	29.898320046644177	23.725867358337375	26.00290436308353	20.372908231934915
12	19.374363138019913	27.290114812251726	29.718091511958022	23.617430537770343
13	27.9397821455233	21.13537165494205	27.66623800519843	23.25860819433622
14	21.143000375986965	23.2692339129345	26.827487371742126	28.76027833933641
15	23.35628306914346	29.604886740737918	23.493463820790446	23.545366369328182
16	24.287531945269375	28.464937853168916	24.00581417525352	23.24171602630819
17	20.30138897213882	31.45880218181422	25.381027370761295	22.85878147528567
18	22.37844450377894	27.33615959284426	26.441692050327763	23.843703853049036
19	23.227275947187454	29.341423410365252	26.555714184516965	20.87558645793033
20	23.90132794237046	26.245388710582674	26.760054926791522	23.093228420255343
21	24.16533618138919	26.939874779993133	25.8029228899775	23.09186614864018
22	20.357787017006597	31.134853991728285	26.182587989123622	22.324771002141492
23	22.71070255071737	28.047674057444272	26.453135131895138	22.78848825994322
24	24.224867450971846	29.416893257845324	25.749113161178528	20.609126130004306
25	22.00191262934769	30.410534173945734	26.080281390824826	21.507271805881743
26	23.452868126658565	29.968477034825113	25.93233869341805	20.64631614509827
27	21.842254396050503	30.072145904739074	26.545633374564755	21.539966324645672
28	21.573478206378702	27.90245590326782	28.000539459559604	22.52352643079388
29	21.675512350354463	28.802644986568005	26.513347537285377	23.008495125792162
30	22.17015317382041	29.0722385392089	27.968526076603258	20.789082210367432
31	24.786123356419296	27.024063165810254	26.11106872932753	22.078744748442926
32	21.467357247557448	28.645575069339625	27.617468681375566	22.26959900172736
33	20.24090411242555	30.08672221102132	28.913261441719296	20.75911223483383
34	22.26033555474425	29.35790689690873	27.042726286937995	21.339031261409026
35	20.939885678166057	28.60184615049287	30.263817520992603	20.194450650348468
36	22.390568721153898	28.667507642343757	25.752246385893407	23.189677250608938
37	21.691587155413394	27.31558929145529	28.083501800923077	22.909321752208243
38	21.857648065301852	26.060528452404952	28.63862748410229	23.4431959981909
39	23.40505239296632	26.55707645613213	27.401276176049084	22.636594974852468
40	20.660483769895976	25.704975560847227	31.85767531073416	21.77686535852264
41	21.662162088525857	26.888789594424495	27.13331734934638	24.315730967703264
42	22.30229352049129	25.77594991199725	30.24678912580306	21.674967441708397
43	22.002729992316787	25.350103805097074	29.989456017698636	22.657710184887502
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	12.0
2	22.0
3	82.0
4	142.0
5	142.0
6	192.0
7	242.0
8	239.5
9	237.0
10	405.0
11	573.0
12	573.0
13	816.5
14	1060.0
15	2602.5
16	4145.0
17	4702.5
18	5260.0
19	5260.0
20	5888.5
21	6517.0
22	8278.5
23	10040.0
24	12422.5
25	14805.0
26	14805.0
27	18113.0
28	21421.0
29	26117.0
30	30813.0
31	33173.0
32	35533.0
33	35533.0
34	38525.5
35	41518.0
36	43493.0
37	45468.0
38	48210.0
39	50952.0
40	50952.0
41	52879.5
42	54807.0
43	52253.0
44	49699.0
45	50256.0
46	50813.0
47	50813.0
48	51050.5
49	51288.0
50	61471.0
51	71654.0
52	59270.0
53	46886.0
54	46886.0
55	49601.0
56	52316.0
57	43793.0
58	35270.0
59	26476.5
60	17683.0
61	17683.0
62	15209.5
63	12736.0
64	10700.0
65	8664.0
66	7112.0
67	5560.0
68	5560.0
69	4432.0
70	3304.0
71	2746.0
72	2188.0
73	1692.5
74	1197.0
75	1197.0
76	919.0
77	641.0
78	497.0
79	353.0
80	251.0
81	149.0
82	149.0
83	112.5
84	76.0
85	47.0
86	18.0
87	14.0
88	10.0
89	10.0
90	7.0
91	4.0
92	3.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	734068.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.16485760746593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.42687583592846	42.99786241009482
2	10.787436714222544	11.254502002939383
3	2.7130418331556214	4.2457632272898405
4	1.0691446606445503	2.230871159372218
5	0.6294784101738269	1.6418325816845856
6	0.3850924849098468	1.205297678461643
7	0.26257306656800217	0.9587960640352854
8	0.20133622127157608	0.8402140251085615
9	0.16389930404085	0.7694805471528335
>10	1.122357647095057	11.675658806575814
>50	0.14644883276445184	5.357186074808984
>100	0.08499255381927748	7.719417665467637
>500	0.004707279903822902	1.7128796558767077
>1k	0.0015690933012743008	1.9600712723507914
>5k	2.6151555021238345E-4	0.7631290852958189
>10k+	7.845466506371504E-4	4.66703774348503
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	12661	1.724772091958783	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	11091	1.5108954483780794	RNA PCR Primer, Index 17 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG	10459	1.4247998822997325	No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC	5594	0.7620547415225837	No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC	4629	0.630595530659285	RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	3242	0.44164845763607735	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2086	0.28416985892315155	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2004	0.2729992316788091	No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG	1308	0.1781851272634143	No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA	1099	0.1497136505064926	No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT	918	0.1250565342720293	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	900	0.1226044453647346	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	881	0.12001612929592353	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	785	0.10693832179035186	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	781	0.10639341314428637	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	768	0.10462246004457354	No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT	764	0.10407755139850805	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	742	0.10108055384514786	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.0868148454911534E-4	0.0
11	0.0	0.0	0.0	4.0868148454911534E-4	0.0
12	0.0	0.0	0.0	5.449086460654871E-4	0.0
13	0.0	0.0	0.0	5.449086460654871E-4	0.0
14	0.0	0.0	0.0	6.81135807581859E-4	0.0
15	0.0	0.0	0.0	6.81135807581859E-4	0.0
16	0.0	0.0	0.0	9.535901306146025E-4	0.0
17	0.0	0.0	0.0	0.0010898172921309742	0.0
18	0.0	0.0	0.0	0.0010898172921309742	0.0
19	0.0	0.0	0.0	0.001226044453647346	0.0
20	0.0	0.0	0.0	0.0014984987766800896	0.0
21	0.0	0.0	0.0	0.001770953099712833	0.0
22	0.0	0.0	0.0	0.0020434074227455765	0.0
23	0.0	0.0	0.0	0.002588316068811064	0.0
24	0.0	0.0	0.0	0.0029969975533601792	0.0
25	0.0	0.0	0.0	0.0034056790379092946	0.0
26	0.0	0.0	0.0	0.0039505876839747815	0.0
27	0.0	0.0	0.0	0.004767950653073012	0.0
28	0.0	0.0	0.0	0.009399674144629652	0.0
29	0.0	0.0	0.0	0.017981985320161074	0.0
30	0.0	0.0	0.0	0.03201338295634737	0.0
31	0.0	0.0	0.0	0.0681135807581859	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	55	5.002221E-10	33.636364	1
CTTATAC	1620	0.0	33.117283	1
CGTCTTC	635	0.0	32.047245	37
TTATACA	1855	0.0	29.819407	2
GGTATCA	325	0.0	29.6	1
TATACAC	1930	0.0	28.660622	3
TTACGGG	65	9.3821654E-8	25.615385	3
TGCACGC	60	1.3363078E-6	24.666666	37
CCGTCTT	1695	0.0	23.684366	37
GCCGTCT	1735	0.0	23.13833	36
AACGGCC	40	0.0019305256	23.125002	37
GTATCAA	980	0.0	22.27551	1
TCGCCGA	45	0.0038245101	20.555557	1
TCGGCGT	45	0.0038245101	20.555557	11
CGCCGAG	45	0.0038245101	20.555557	2
GCACCGC	65	6.897788E-5	19.923077	10
TGCCGTC	2100	0.0	19.116667	35
TCGCCAT	120	5.164111E-9	18.5	13
CCGATCT	70	1.2184361E-4	18.5	9
AGCGGAG	60	9.2324836E-4	18.5	10
>>END_MODULE