FastQCFastQC Report
Fri 10 Feb 2017
ERR1630934.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630934.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919440
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT218232.373509962585922No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG177371.9291090228834944No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT154631.6817845645175324TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC98131.0672800835291045No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC58610.6374532324023319RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC45500.49486644044200817TruSeq Adapter, Index 11 (95% over 21bp)
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG23710.25787435830505523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20290.220677803880623No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20280.22056904202558078No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA16750.18217610719568433No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT13990.15215783520403722No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT12220.1329069868615679No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9380.10201862002958322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC26550.033.7250481
TTATACA29000.031.0672422
TATACAC29700.030.7710443
CGTCTTC7800.026.32692337
CCGTCTT23100.025.86796637
GCCGTCT23650.025.26638436
GATTACG601.3369208E-624.6666661
GGTATCA4350.024.241381
GTATCAA11450.021.6506541
ATGCACG705.1000916E-621.14285736
TGCCGTC29250.020.4290635
TCGCCGA555.1420165E-420.1818181
ATGCCGT30850.019.3695334
ATACACA50150.018.8504494
TACACAT50350.018.7020855
TAAGTCG609.234901E-418.522
TATGCCG33700.017.73145333
GAATAAG2100.017.6190471
CTAGGAC1054.794674E-717.6190473
TCGATAA650.001579608817.07692318
Produced by FastQC (version 0.11.5)