##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630934.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	919440
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43786543983294	31.0	31.0	34.0	28.0	34.0
2	31.45190768293744	31.0	31.0	34.0	28.0	34.0
3	30.932511093709213	31.0	31.0	34.0	27.0	34.0
4	35.080432654659354	37.0	35.0	37.0	32.0	37.0
5	35.25678021404333	37.0	35.0	37.0	33.0	37.0
6	35.441126120247105	37.0	35.0	37.0	33.0	37.0
7	35.44466958148438	37.0	35.0	37.0	33.0	37.0
8	35.471839380492476	37.0	35.0	37.0	33.0	37.0
9	37.25675737405377	39.0	37.0	39.0	34.0	39.0
10	37.08243496041069	39.0	37.0	39.0	33.0	39.0
11	37.14290220133994	39.0	37.0	39.0	33.0	39.0
12	37.068938701818496	39.0	37.0	39.0	33.0	39.0
13	37.15022404942139	39.0	37.0	39.0	33.0	39.0
14	38.26889845993213	40.0	38.0	41.0	33.0	41.0
15	38.27788653963282	40.0	38.0	41.0	33.0	41.0
16	38.276730401113724	40.0	38.0	41.0	33.0	41.0
17	38.217736883320285	40.0	38.0	41.0	33.0	41.0
18	38.2913719220395	40.0	38.0	41.0	33.0	41.0
19	38.29531562690333	40.0	38.0	41.0	34.0	41.0
20	38.273816671017144	40.0	38.0	41.0	34.0	41.0
21	38.19289785086574	40.0	38.0	41.0	33.0	41.0
22	38.081553989384844	40.0	37.0	41.0	33.0	41.0
23	38.125712390150525	40.0	37.0	41.0	33.0	41.0
24	38.080753502131735	40.0	37.0	41.0	33.0	41.0
25	38.04945836596189	40.0	37.0	41.0	33.0	41.0
26	37.945865961889844	40.0	37.0	41.0	33.0	41.0
27	37.83326046289046	40.0	37.0	41.0	33.0	41.0
28	37.84100756982511	40.0	37.0	41.0	33.0	41.0
29	37.73605564256504	40.0	37.0	41.0	32.0	41.0
30	37.69394849038545	40.0	37.0	41.0	32.0	41.0
31	37.659683067954404	40.0	37.0	41.0	32.0	41.0
32	37.526990341947275	39.0	37.0	41.0	32.0	41.0
33	37.55290829200383	39.0	37.0	41.0	32.0	41.0
34	37.541537240059164	39.0	37.0	41.0	32.0	41.0
35	37.550598190202734	40.0	37.0	41.0	32.0	41.0
36	37.5376381275559	40.0	37.0	41.0	32.0	41.0
37	37.49633146262943	40.0	37.0	41.0	32.0	41.0
38	37.45118224136431	39.0	37.0	41.0	32.0	41.0
39	37.36047050378491	39.0	37.0	41.0	31.0	41.0
40	37.24966392586792	39.0	36.0	41.0	31.0	41.0
41	37.224190811798486	39.0	36.0	41.0	31.0	41.0
42	37.181063473418604	39.0	36.0	41.0	31.0	41.0
43	36.491013007917864	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	15.0
10	18.0
11	18.0
12	18.0
13	16.0
14	9.0
15	15.0
16	20.0
17	15.0
18	22.0
19	35.0
20	82.0
21	171.0
22	391.0
23	672.0
24	1321.0
25	2155.0
26	3573.0
27	5379.0
28	7960.0
29	11450.0
30	15477.0
31	20794.0
32	27174.0
33	35156.0
34	46143.0
35	61943.0
36	85819.0
37	126817.0
38	222134.0
39	244621.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.58988079700688	22.71165056991212	15.338466892891326	25.36000174018968
2	13.309732010789176	21.40085269294353	41.424345253632644	23.86507004263465
3	14.18852779953015	28.351387801270338	34.719829461411294	22.74025493778822
4	11.504393978943705	19.63107978769686	36.47676846776299	32.38775776559645
5	12.611372139563212	37.778647872618116	35.650939702427564	13.959040285391108
6	27.937331419124682	39.55048725311059	17.570042634647177	14.942138693117549
7	23.97100408944575	33.627207865657354	22.132058644392238	20.269729400504655
8	24.739297833463848	35.095057861306884	22.485860958844516	17.679783346384756
9	23.260897937875228	14.797920473331594	19.151222483250674	42.7899591055425
10	14.24791177238319	28.581201600974502	33.43034890803098	23.74053771861133
11	31.019424867310537	22.44366135908814	25.499760723918907	21.037153049682416
12	19.93278517358392	26.23031410423736	28.88160184460106	24.955298877577654
13	29.175041329504914	20.22002523275037	26.691246845906203	23.91368659183851
14	21.48623074915166	22.37383624815105	25.803858870616896	30.336074132080398
15	24.060406334290438	28.634821195510312	21.965217958757506	25.339554511441747
16	25.54522317932655	26.90431131993387	23.03510832680762	24.51535717393196
17	20.533476898981988	31.420973636126337	24.414099886887673	23.631449578004002
18	24.818258940224485	25.066018446010613	25.043939789437047	25.071782824327855
19	25.42177847385365	27.481184199077703	24.771491342556338	22.325545984512313
20	23.679087270512486	26.28121465239711	24.72950926651005	25.310188810580353
21	25.283542156095013	26.909966936396067	24.846645784390496	22.95984512311842
22	23.172800835291046	30.284738536500477	24.92234403549987	21.620116592708605
23	23.318541721047595	25.19642391020621	25.78776211607065	25.69727225267554
24	25.070477682067345	27.81997737753415	26.303402070825722	20.80614286957278
25	22.333594361785437	29.38451666231619	24.93213260245367	23.349756373444706
26	25.58535630383712	26.91975550334986	25.15009135995824	22.344796832854783
27	24.18809275210998	29.541242495432	25.3569564082485	20.91370834420952
28	20.944596711041505	26.89136865918385	26.899417036456974	25.264617593317674
29	25.775472026450885	25.582746019316104	26.387801270338468	22.253980683894543
30	23.488210214913423	26.170060036543987	26.97533281127643	23.366396937266163
31	22.16131558339859	27.061254676759766	26.833181066736273	23.944248673105367
32	24.389737231358218	27.828460802227443	26.645675628643524	21.136126337770815
33	20.54522317932655	25.291155485947968	28.30733924997825	25.85628208474724
34	21.042699904289567	28.315061341686242	28.492343165405032	22.14989558861916
35	21.23520838771426	27.49053771861133	30.365983642217003	20.90827025145741
36	22.96919864265205	25.35217088662664	24.81118941964674	26.86744105107457
37	21.92236578787088	26.930522926999046	27.779082920038284	23.368028365091796
38	21.842534586269906	24.706125467675978	28.586204646306445	24.865135299747674
39	24.484795092665102	25.42438875837466	26.190289741581836	23.900526407378404
40	21.019316105455495	24.386583137561992	32.48749238667015	22.106608370312365
41	22.47639867745584	25.986687548942832	25.882928739232575	25.65398503436875
42	22.92134342643348	24.580070477682067	30.1749978247629	22.32358827112155
43	23.033911946402156	24.131971634908204	29.698947185243192	23.135169233446444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	10.0
2	17.0
3	63.0
4	109.0
5	109.0
6	167.5
7	226.0
8	240.0
9	254.0
10	331.0
11	408.0
12	408.0
13	692.0
14	976.0
15	2282.0
16	3588.0
17	4122.5
18	4657.0
19	4657.0
20	4948.0
21	5239.0
22	6621.5
23	8004.0
24	10456.0
25	12908.0
26	12908.0
27	16599.5
28	20291.0
29	24313.5
30	28336.0
31	32736.5
32	37137.0
33	37137.0
34	42063.0
35	46989.0
36	50381.0
37	53773.0
38	57204.5
39	60636.0
40	60636.0
41	62252.0
42	63868.0
43	65172.0
44	66476.0
45	66575.5
46	66675.0
47	66675.0
48	65547.0
49	64419.0
50	63901.0
51	63383.0
52	79391.5
53	95400.0
54	95400.0
55	72486.5
56	49573.0
57	57162.5
58	64752.0
59	53467.0
60	42182.0
61	42182.0
62	31690.0
63	21198.0
64	17635.5
65	14073.0
66	11761.5
67	9450.0
68	9450.0
69	7795.5
70	6141.0
71	4898.0
72	3655.0
73	2863.0
74	2071.0
75	2071.0
76	1620.0
77	1169.0
78	933.0
79	697.0
80	549.0
81	401.0
82	401.0
83	279.5
84	158.0
85	122.5
86	87.0
87	64.5
88	42.0
89	42.0
90	28.0
91	14.0
92	9.0
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	919440.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.12019967958716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.45221696859151	44.02754081583652
2	12.96519076648315	14.552181893979482
3	3.9118928930612045	6.586086308512582
4	1.623857512490246	3.6452483140860124
5	0.8348068675191281	2.3424764049532065
6	0.4854991595846127	1.6347785868096134
7	0.32852569184769437	1.290584919845699
8	0.22427397372952362	1.0069040150908282
9	0.17503174768609786	0.8840534967369837
>10	0.9044053851833864	9.315830367510653
>50	0.0631896600176727	2.457914063129468
>100	0.027824533130162102	2.5733605504172434
>500	9.661296104907293E-4	0.36045048249641076
>1k	0.001352581454687021	1.6562938236011366
>5k	3.8645184419629176E-4	1.699669332926818
>10k+	5.796777662944376E-4	5.966626624067391
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	21823	2.373509962585922	No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG	17737	1.9291090228834944	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	15463	1.6817845645175324	TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC	9813	1.0672800835291045	No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC	5861	0.6374532324023319	RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	4550	0.49486644044200817	TruSeq Adapter, Index 11 (95% over 21bp)
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG	2371	0.25787435830505523	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2029	0.220677803880623	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2028	0.22056904202558078	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA	1675	0.18217610719568433	No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT	1399	0.15215783520403722	No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT	1222	0.1329069868615679	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	938	0.10201862002958322	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.175237100843992E-4	0.0
11	0.0	0.0	0.0	2.175237100843992E-4	0.0
12	0.0	0.0	0.0	2.175237100843992E-4	0.0
13	0.0	0.0	0.0	2.175237100843992E-4	0.0
14	0.0	0.0	0.0	2.175237100843992E-4	0.0
15	0.0	0.0	0.0	2.175237100843992E-4	0.0
16	0.0	0.0	0.0	3.262855651265988E-4	0.0
17	0.0	0.0	0.0	4.350474201687984E-4	0.0
18	0.0	0.0	0.0	4.350474201687984E-4	0.0
19	0.0	0.0	0.0	5.43809275210998E-4	0.0
20	0.0	0.0	0.0	8.700948403375968E-4	0.0
21	0.0	0.0	0.0	0.001087618550421996	0.0
22	0.0	0.0	0.0	0.0011963804054641956	0.0
23	0.0	0.0	0.0	0.0013051422605063951	0.0
24	0.0	0.0	0.0	0.001413904115548595	0.0
25	0.0	0.0	0.0	0.0015226659705907945	0.0
26	0.0	0.0	0.0	0.001631427825632994	0.0
27	0.0	0.0	0.0	0.0020664752458017925	0.0
28	0.0	0.0	0.0	0.007722091707996172	0.0
29	0.0	0.0	0.0	0.019250848342469328	0.0
30	0.0	0.0	0.0	0.03523884103367267	0.0
31	0.0	0.0	0.0	0.07297920473331593	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2655	0.0	33.725048	1
TTATACA	2900	0.0	31.067242	2
TATACAC	2970	0.0	30.771044	3
CGTCTTC	780	0.0	26.326923	37
CCGTCTT	2310	0.0	25.867966	37
GCCGTCT	2365	0.0	25.266384	36
GATTACG	60	1.3369208E-6	24.666666	1
GGTATCA	435	0.0	24.24138	1
GTATCAA	1145	0.0	21.650654	1
ATGCACG	70	5.1000916E-6	21.142857	36
TGCCGTC	2925	0.0	20.42906	35
TCGCCGA	55	5.1420165E-4	20.181818	1
ATGCCGT	3085	0.0	19.36953	34
ATACACA	5015	0.0	18.850449	4
TACACAT	5035	0.0	18.702085	5
TAAGTCG	60	9.234901E-4	18.5	22
TATGCCG	3370	0.0	17.731453	33
GAATAAG	210	0.0	17.619047	1
CTAGGAC	105	4.794674E-7	17.619047	3
TCGATAA	65	0.0015796088	17.076923	18
>>END_MODULE